Restriction map for
pF1KB6555
(Linear) (Six-base) MAPSORT of: KIBB6555.seq Check: 5069 from: 1 to: 1116
KIBB6555.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 584 1116
Size: 584 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1116
Size: 380 736
AleI CACnn'nnGTG
Cuts at: 0 811 1116
Size: 811 305
AseI AT'TA_AT
Cuts at: 0 301 314 1116
Size: 301 13 802
Fragments arranged by size:
802 301 13
AvaI C'yCGr_G
Cuts at: 0 208 1116
Size: 208 908
BamHI G'GATC_C
Cuts at: 0 252 1116
Size: 252 864
BanII G_rGCy'C
Cuts at: 0 905 998 1116
Size: 905 93 118
Fragments arranged by size:
905 118 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 1046 1116
Size: 1046 70
BbvCI CC'TCA_GC
Cuts at: 0 181 431 1116
Size: 181 250 685
Fragments arranged by size:
685 250 181
Bme1580I G_kGCm'C
Cuts at: 0 926 1116
Size: 926 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 858 1116
Size: 435 423 258
Fragments arranged by size:
435 423 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 427 502 658 862 928 1116
Size: 244 183 75 156 204 66 188
Fragments arranged by size:
244 204 188 183 156 75 66
Bpu10I CC'TnA_GC
Cuts at: 0 181 431 1116
Size: 181 250 685
Fragments arranged by size:
685 250 181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 241 1116
Size: 166 75 875
Fragments arranged by size:
875 166 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1116
Size: 913 203
BsiHKAI G_wGCw'C
Cuts at: 0 998 1116
Size: 998 118
Bsp1286I G_dGCh'C
Cuts at: 0 905 926 998 1116
Size: 905 21 72 118
Fragments arranged by size:
905 118 72 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 584 1116
Size: 182 402 532
Fragments arranged by size:
532 402 182
BsrGI T'GTAC_A
Cuts at: 0 1082 1116
Size: 1082 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 1116
Size: 468 648
BstEII G'GTnAC_C
Cuts at: 0 435 1116
Size: 435 681
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 642 1116
Size: 558 84 474
Fragments arranged by size:
558 474 84
BstYI r'GATC_y
Cuts at: 0 252 1116
Size: 252 864
BtgI C'CryG_G
Cuts at: 0 1030 1116
Size: 1030 86
BtsI GCAGTG_nn'
Cuts at: 0 273 1116
Size: 273 843
DraIII CAC_nnn'GTG
Cuts at: 0 268 1116
Size: 268 848
EaeI y'GGCC_r
Cuts at: 0 2 563 1033 1116
Size: 2 561 470 83
Fragments arranged by size:
561 470 83 2
EarI CTCTTCn'nnn_
Cuts at: 0 1096 1116
Size: 1096 20
EcoICRI GAG'CTC
Cuts at: 0 996 1116
Size: 996 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 380 427 502 658 862 928 1116
Size: 244 136 47 75 156 204 66 188
Fragments arranged by size:
244 204 188 156 136 75 66 47
EcoO109I rG'GnC_Cy
Cuts at: 0 104 1116
Size: 104 1012
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 323 355 653 685 1048 1080
Size: 244 32 47 32 298 32 363 32
Cuts at: 1080 1116
Size: 36
Fragments arranged by size:
363 298 244 47 36 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1050 1116
Size: 1050 66
HindIII A'AGCT_T
Cuts at: 0 277 391 1116
Size: 277 114 725
Fragments arranged by size:
725 277 114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 995 1116
Size: 309 686 121
Fragments arranged by size:
686 309 121
MscI TGG'CCA
Cuts at: 0 4 565 1116
Size: 4 561 551
Fragments arranged by size:
561 551 4
MslI CAynn'nnrTG
Cuts at: 0 640 737 811 1116
Size: 640 97 74 305
Fragments arranged by size:
640 305 97 74
MspA1I CmG'CkG
Cuts at: 0 1032 1116
Size: 1032 84
PfoI T'CCnGG_A
Cuts at: 0 479 724 905 1116
Size: 479 245 181 211
Fragments arranged by size:
479 245 211 181
PpuMI rG'GwC_Cy
Cuts at: 0 104 1116
Size: 104 1012
PstI C_TGCA'G
Cuts at: 0 149 389 473 1116
Size: 149 240 84 643
Fragments arranged by size:
643 240 149 84
SacI G_AGCT'C
Cuts at: 0 998 1116
Size: 998 118
SacII CC_GC'GG
Cuts at: 0 1033 1116
Size: 1033 83
SanDI GG'GwC_CC
Cuts at: 0 104 1116
Size: 104 1012
SexAI A'CCwGG_T
Cuts at: 0 884 1116
Size: 884 232
SfcI C'TryA_G
Cuts at: 0 145 385 469 1116
Size: 145 240 84 647
Fragments arranged by size:
647 240 145 84
StyI C'CwwG_G
Cuts at: 0 128 1116
Size: 128 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 1116
Size: 454 662
TatI w'GTAC_w
Cuts at: 0 1082 1116
Size: 1082 34
Tth111I GACn'n_nGTC
Cuts at: 0 1045 1116
Size: 1045 71
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 558 871 1116
Size: 558 313 245
Fragments arranged by size:
558 313 245
XmnI GAAnn'nnTTC
Cuts at: 0 394 1116
Size: 394 722
Enzymes that do cut:
AarI AcuI AleI AseI AvaI BamHI BanII BbsI
BbvCI Bme1580I BmrI BpmI Bpu10I BseRI BsgI BsiHKAI
Bsp1286I BspMI BsrGI BstAPI BstEII BstXI BstYI BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I
HincII HindIII MmeI MscI MslI MspA1I PfoI PpuMI
PstI SacI SacII SanDI SexAI SfcI StyI TaqII
TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XhoI XmaI ZraI