Restriction map for
pF1KB6561
(Linear) (Six-base) MAPSORT of: KIBB6561.seq Check: 1692 from: 1 to: 1122
KIBB6561.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AflII C'TTAA_G
Cuts at: 0 808 1122
Size: 808 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 721 1122
Size: 721 401
AlwNI CAG_nnn'CTG
Cuts at: 0 308 1122
Size: 308 814
ApaLI G'TGCA_C
Cuts at: 0 1093 1122
Size: 1093 29
ApoI r'AATT_y
Cuts at: 0 447 1122
Size: 447 675
BanI G'GyrC_C
Cuts at: 0 251 490 1122
Size: 251 239 632
Fragments arranged by size:
632 251 239
BanII G_rGCy'C
Cuts at: 0 1109 1122
Size: 1109 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 764 1034 1122
Size: 330 434 270 88
Fragments arranged by size:
434 330 270 88
BclI T'GATC_A
Cuts at: 0 68 1122
Size: 68 1054
BfrBI ATG'CAT
Cuts at: 0 586 1122
Size: 586 536
Bme1580I G_kGCm'C
Cuts at: 0 1097 1122
Size: 1097 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 557 628 832 1122
Size: 312 245 71 204 290
Fragments arranged by size:
312 290 245 204 71
Bpu10I CC'TnA_GC
Cuts at: 0 504 628 1122
Size: 504 124 494
Fragments arranged by size:
504 494 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1122
Size: 384 738
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 330 1122
Size: 268 62 792
Fragments arranged by size:
792 268 62
BsaAI yAC'GTr
Cuts at: 0 940 1122
Size: 940 182
BsaWI w'CCGG_w
Cuts at: 0 1097 1122
Size: 1097 25
BseYI C'CCAG_C
Cuts at: 0 202 1122
Size: 202 920
BsiHKAI G_wGCw'C
Cuts at: 0 137 1097 1122
Size: 137 960 25
Fragments arranged by size:
960 137 25
BsmI GAATG_Cn'
Cuts at: 0 588 1122
Size: 588 534
Bsp1286I G_dGCh'C
Cuts at: 0 137 1097 1109 1122
Size: 137 960 12 13
Fragments arranged by size:
960 137 13 12
BspHI T'CATG_A
Cuts at: 0 996 1122
Size: 996 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 312 1122
Size: 312 810
BsrDI GCAATG_nn'
Cuts at: 0 498 1122
Size: 498 624
BsrGI T'GTAC_A
Cuts at: 0 395 1122
Size: 395 727
BstEII G'GTnAC_C
Cuts at: 0 621 1122
Size: 621 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 145 291 1122
Size: 145 146 831
Fragments arranged by size:
831 146 145
BtgI C'CryG_G
Cuts at: 0 85 1122
Size: 85 1037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 505 1122
Size: 505 617
BtsI GCAGTG_nn'
Cuts at: 0 71 899 1122
Size: 71 828 223
Fragments arranged by size:
828 223 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 219 254 1122
Size: 219 35 868
Fragments arranged by size:
868 219 35
EarI CTCTTCn'nnn_
Cuts at: 0 747 1040 1122
Size: 747 293 82
Fragments arranged by size:
747 293 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 557 628 832 1122
Size: 312 245 71 204 290
Fragments arranged by size:
312 290 245 204 71
EcoRI G'AATT_C
Cuts at: 0 447 1122
Size: 447 675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 1122
Size: 666 456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1122
Size: 806 316
MspA1I CmG'CkG
Cuts at: 0 291 1080 1122
Size: 291 789 42
Fragments arranged by size:
789 291 42
NcoI C'CATG_G
Cuts at: 0 85 1122
Size: 85 1037
NsiI A_TGCA'T
Cuts at: 0 588 1122
Size: 588 534
PasI CC'CwG_GG
Cuts at: 0 1110 1122
Size: 1110 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 40 1122
Size: 40 1082
PfoI T'CCnGG_A
Cuts at: 0 605 1122
Size: 605 517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 1122
Size: 709 32 381
Fragments arranged by size:
709 381 32
PstI C_TGCA'G
Cuts at: 0 323 1085 1122
Size: 323 762 37
Fragments arranged by size:
762 323 37
PvuII CAG'CTG
Cuts at: 0 291 1080 1122
Size: 291 789 42
Fragments arranged by size:
789 291 42
SapI GCTCTTCn'nnn_
Cuts at: 0 1040 1122
Size: 1040 82
SfcI C'TryA_G
Cuts at: 0 207 319 1081 1122
Size: 207 112 762 41
Fragments arranged by size:
762 207 112 41
SmlI C'TyrA_G
Cuts at: 0 363 808 1122
Size: 363 445 314
Fragments arranged by size:
445 363 314
SnaBI TAC'GTA
Cuts at: 0 940 1122
Size: 940 182
StuI AGG'CCT
Cuts at: 0 660 1122
Size: 660 462
StyI C'CwwG_G
Cuts at: 0 85 1122
Size: 85 1037
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 918 1122
Size: 918 204
TatI w'GTAC_w
Cuts at: 0 395 1122
Size: 395 727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 863 1122
Size: 863 259
Enzymes that do cut:
AflII AlfI AlwNI ApaLI ApoI BanI BanII BbsI
BclI BfrBI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstEII BstXI BtgI BtgZI BtsI CspCI EarI
Eco57MI EcoRI FalI MmeI MspA1I NcoI NsiI PasI
PflMI PfoI PpiI PstI PvuII SapI SfcI SmlI
SnaBI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BglI BglII BlpI BmgBI BmrI BmtI BplI BsaBI
BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I ClaI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI