Restriction map for
pF1KB6562
(Linear) (Six-base) MAPSORT of: KIBB6562.seq Check: 6935 from: 1 to: 1197
KIBB6562.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 544 1197
Size: 544 653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 280 489 1197
Size: 151 129 209 708
Fragments arranged by size:
708 209 151 129
ApaLI G'TGCA_C
Cuts at: 0 497 1197
Size: 497 700
ApoI r'AATT_y
Cuts at: 0 138 395 608 1003 1197
Size: 138 257 213 395 194
Fragments arranged by size:
395 257 213 194 138
AvaI C'yCGr_G
Cuts at: 0 15 1011 1197
Size: 15 996 186
Fragments arranged by size:
996 186 15
BanI G'GyrC_C
Cuts at: 0 55 81 810 1197
Size: 55 26 729 387
Fragments arranged by size:
729 387 55 26
BanII G_rGCy'C
Cuts at: 0 16 1197
Size: 16 1181
BbeI G_GCGC'C
Cuts at: 0 85 1197
Size: 85 1112
BclI T'GATC_A
Cuts at: 0 942 1197
Size: 942 255
BglI GCCn_nnn'nGGC
Cuts at: 0 20 1197
Size: 20 1177
BglII A'GATC_T
Cuts at: 0 263 1197
Size: 263 934
Bme1580I G_kGCm'C
Cuts at: 0 501 1197
Size: 501 696
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 1197
Size: 291 906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1197
Size: 891 306
BsaHI Gr'CG_yC
Cuts at: 0 82 1197
Size: 82 1115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 344 1197
Size: 344 853
BseYI C'CCAG_C
Cuts at: 0 1174 1197
Size: 1174 23
BsiEI CG_ry'CG
Cuts at: 0 98 1197
Size: 98 1099
BsiHKAI G_wGCw'C
Cuts at: 0 501 1197
Size: 501 696
Bsp1286I G_dGCh'C
Cuts at: 0 16 501 1197
Size: 16 485 696
Fragments arranged by size:
696 485 16
BsrBI CCG'CTC
Cuts at: 0 20 860 1197
Size: 20 840 337
Fragments arranged by size:
840 337 20
BstYI r'GATC_y
Cuts at: 0 263 524 969 1197
Size: 263 261 445 228
Fragments arranged by size:
445 263 261 228
BtsI GCAGTG_nn'
Cuts at: 0 290 1197
Size: 290 907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 351 386 1197
Size: 351 35 811
Fragments arranged by size:
811 351 35
EaeI y'GGCC_r
Cuts at: 0 95 899 1197
Size: 95 804 298
Fragments arranged by size:
804 298 95
EagI C'GGCC_G
Cuts at: 0 95 1197
Size: 95 1102
EarI CTCTTCn'nnn_
Cuts at: 0 508 1197
Size: 508 689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 280 489 891 1197
Size: 151 129 209 402 306
Fragments arranged by size:
402 306 209 151 129
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1045 1197
Size: 1045 152
EcoO109I rG'GnC_Cy
Cuts at: 0 74 1197
Size: 74 1123
EcoRI G'AATT_C
Cuts at: 0 395 1003 1197
Size: 395 608 194
Fragments arranged by size:
608 395 194
EcoRV GAT'ATC
Cuts at: 0 687 1197
Size: 687 510
HaeII r_GCGC'y
Cuts at: 0 85 1197
Size: 85 1112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 839 844 871 876 1197
Size: 839 5 27 5 321
Fragments arranged by size:
839 321 27 5 5
HincII GTy'rAC
Cuts at: 0 348 404 1032 1197
Size: 348 56 628 165
Fragments arranged by size:
628 348 165 56
KasI G'GCGC_C
Cuts at: 0 81 1197
Size: 81 1116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1197
Size: 502 695
MscI TGG'CCA
Cuts at: 0 901 1197
Size: 901 296
MspA1I CmG'CkG
Cuts at: 0 47 53 981 1197
Size: 47 6 928 216
Fragments arranged by size:
928 216 47 6
NarI GG'CG_CC
Cuts at: 0 82 1197
Size: 82 1115
NspI r_CATG'y
Cuts at: 0 111 1136 1197
Size: 111 1025 61
Fragments arranged by size:
1025 111 61
PfoI T'CCnGG_A
Cuts at: 0 1151 1197
Size: 1151 46
PpuMI rG'GwC_Cy
Cuts at: 0 74 1197
Size: 74 1123
PstI C_TGCA'G
Cuts at: 0 297 1197
Size: 297 900
PvuII CAG'CTG
Cuts at: 0 981 1197
Size: 981 216
SanDI GG'GwC_CC
Cuts at: 0 74 1197
Size: 74 1123
SfcI C'TryA_G
Cuts at: 0 164 194 293 795 1197
Size: 164 30 99 502 402
Fragments arranged by size:
502 402 164 99 30
SfoI GGC'GCC
Cuts at: 0 83 1197
Size: 83 1114
SphI G_CATG'C
Cuts at: 0 111 1136 1197
Size: 111 1025 61
Fragments arranged by size:
1025 111 61
SspI AAT'ATT
Cuts at: 0 702 726 1197
Size: 702 24 471
Fragments arranged by size:
702 471 24
StyI C'CwwG_G
Cuts at: 0 312 1197
Size: 312 885
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 389 1197
Size: 389 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 1197
Size: 746 32 419
Fragments arranged by size:
746 419 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 705 1197
Size: 367 338 492
Fragments arranged by size:
492 367 338
Enzymes that do cut:
AccI AcuI ApaLI ApoI AvaI BanI BanII BbeI
BclI BglI BglII Bme1580I BmrI BpmI BsaHI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BsrBI BstYI BtsI CspCI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII KasI MmeI MscI MspA1I NarI
NspI PfoI PpuMI PstI PvuII SanDI SfcI SfoI
SphI SspI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BfrBI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EciI EcoICRI FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI