Restriction map for
pF1KB6567
(Linear) (Six-base) MAPSORT of: KIBB6567.seq Check: 6001 from: 1 to: 1122
KIBB6567.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 395 440 1122
Size: 386 9 45 682
Fragments arranged by size:
682 386 45 9
AflIII A'CryG_T
Cuts at: 0 289 1122
Size: 289 833
AleI CACnn'nnGTG
Cuts at: 0 997 1122
Size: 997 125
AlwNI CAG_nnn'CTG
Cuts at: 0 645 982 994 1122
Size: 645 337 12 128
Fragments arranged by size:
645 337 128 12
ApoI r'AATT_y
Cuts at: 0 745 762 1008 1122
Size: 745 17 246 114
Fragments arranged by size:
745 246 114 17
BanI G'GyrC_C
Cuts at: 0 1073 1122
Size: 1073 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 374 421 1122
Size: 117 257 47 701
Fragments arranged by size:
701 257 117 47
BlpI GC'TnA_GC
Cuts at: 0 673 1122
Size: 673 449
BmgBI CAC'GTC
Cuts at: 0 290 1122
Size: 290 832
BmrI ACTGGGnnnn_n'
Cuts at: 0 411 1122
Size: 411 711
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 1122
Size: 87 1035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 667 838 1122
Size: 367 300 171 284
Fragments arranged by size:
367 300 284 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 1122
Size: 57 1065
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 1122
Size: 340 30 752
Fragments arranged by size:
752 340 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 364 682 835 1122
Size: 96 268 318 153 287
Fragments arranged by size:
318 287 268 153 96
BseYI C'CCAG_C
Cuts at: 0 448 1122
Size: 448 674
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 601 1038 1064 1122
Size: 402 199 437 26 58
Fragments arranged by size:
437 402 199 58 26
BsmI GAATG_Cn'
Cuts at: 0 492 551 1122
Size: 492 59 571
Fragments arranged by size:
571 492 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1097 1122
Size: 1097 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 994 1122
Size: 994 128
BstBI TT'CG_AA
Cuts at: 0 849 1122
Size: 849 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 999 1122
Size: 999 123
BtgI C'CryG_G
Cuts at: 0 119 716 1122
Size: 119 597 406
Fragments arranged by size:
597 406 119
BtsI GCAGTG_nn'
Cuts at: 0 146 992 1122
Size: 146 846 130
Fragments arranged by size:
846 146 130
ClaI AT'CG_AT
Cuts at: 0 866 1122
Size: 866 256
DraI TTT'AAA
Cuts at: 0 48 312 555 609 1122
Size: 48 264 243 54 513
Fragments arranged by size:
513 264 243 54 48
EaeI y'GGCC_r
Cuts at: 0 116 1122
Size: 116 1006
EarI CTCTTCn'nnn_
Cuts at: 0 56 371 659 1119 1122
Size: 56 315 288 460 3
Fragments arranged by size:
460 315 288 56 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 386 395 440 667 838 1122
Size: 367 19 9 45 227 171 284
Fragments arranged by size:
367 284 227 171 45 19 9
EcoO109I rG'GnC_Cy
Cuts at: 0 642 1122
Size: 642 480
EcoRI G'AATT_C
Cuts at: 0 1008 1122
Size: 1008 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 817 909 1122
Size: 817 92 213
Fragments arranged by size:
817 213 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 444 476 1122
Size: 340 32 72 32 646
Fragments arranged by size:
646 340 72 32 32
HincII GTy'rAC
Cuts at: 0 231 776 908 1122
Size: 231 545 132 214
Fragments arranged by size:
545 231 214 132
HpaI GTT'AAC
Cuts at: 0 231 1122
Size: 231 891
MscI TGG'CCA
Cuts at: 0 118 1122
Size: 118 1004
MslI CAynn'nnrTG
Cuts at: 0 395 997 1122
Size: 395 602 125
Fragments arranged by size:
602 395 125
NcoI C'CATG_G
Cuts at: 0 119 1122
Size: 119 1003
NdeI CA'TA_TG
Cuts at: 0 738 1122
Size: 738 384
PflMI CCAn_nnn'nTGG
Cuts at: 0 645 1122
Size: 645 477
PfoI T'CCnGG_A
Cuts at: 0 644 1122
Size: 644 478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 1122
Size: 228 32 862
Fragments arranged by size:
862 228 32
PpuMI rG'GwC_Cy
Cuts at: 0 642 1122
Size: 642 480
PstI C_TGCA'G
Cuts at: 0 153 1122
Size: 153 969
ScaI AGT'ACT
Cuts at: 0 250 1122
Size: 250 872
SfcI C'TryA_G
Cuts at: 0 149 1122
Size: 149 973
SmlI C'TyrA_G
Cuts at: 0 72 1122
Size: 72 1050
SspI AAT'ATT
Cuts at: 0 53 1122
Size: 53 1069
StyI C'CwwG_G
Cuts at: 0 119 884 932 1122
Size: 119 765 48 190
Fragments arranged by size:
765 190 119 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 393 652 1122
Size: 393 259 470
Fragments arranged by size:
470 393 259
TatI w'GTAC_w
Cuts at: 0 248 533 1122
Size: 248 285 589
Fragments arranged by size:
589 285 248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 813 1122
Size: 126 687 309
Fragments arranged by size:
687 309 126
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI BanI BbsI BlpI
BmgBI BmrI BplI BpmI BpuEI BsaXI BseRI BseYI
BsgI BsmI BspMI BstAPI BstBI BstXI BtgI BtsI
ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII HpaI MscI MslI NcoI NdeI PflMI
PfoI PpiI PpuMI PstI ScaI SfcI SmlI SspI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmtI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstYI BstZ17I Bsu36I BtgZI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI