(Linear) (Six-base) MAPSORT of: KIBB6575.seq  Check: 5285  from: 1  to: 1128



KIBB6575.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     445    1128 
   Size:        445     683
 
AccI GT'mk_AC

Cuts at:      0     509    1128 
   Size:        509     619
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523    1128 
   Size:        523     605
 
ApaLI G'TGCA_C

Cuts at:      0     374    1128 
   Size:        374     754
 
ApoI r'AATT_y

Cuts at:      0     365    1128 
   Size:        365     763
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1034    1067    1128 
   Size:       1034      33      61

  Fragments arranged by size: 

                1034      61      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      16    1128 
   Size:         16    1112
 
BbvCI CC'TCA_GC

Cuts at:      0     104    1128 
   Size:        104    1024
 
BclI T'GATC_A

Cuts at:      0     806    1128 
   Size:        806     322
 
BlpI GC'TnA_GC

Cuts at:      0     136    1128 
   Size:        136     992
 
Bme1580I G_kGCm'C

Cuts at:      0     347     378    1128 
   Size:        347      31     750

  Fragments arranged by size: 

                 750     347      31
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     220    1128 
   Size:        220     908
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44    1128 
   Size:         44    1084
 
Bpu10I CC'TnA_GC

Cuts at:      0     104    1128 
   Size:        104    1024
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    1107    1128 
   Size:        292     815      21

  Fragments arranged by size: 

                 815     292      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1103    1128 
   Size:       1103      25
 
BsaBI GATnn'nnATC

Cuts at:      0     875    1128 
   Size:        875     253
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     461    1128 
   Size:        461     667
 
BseYI C'CCAG_C

Cuts at:      0     797     988    1128 
   Size:        797     191     140

  Fragments arranged by size: 

                 797     191     140
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1128 
   Size:        445     683
 
BsiHKAI G_wGCw'C

Cuts at:      0     378    1093    1128 
   Size:        378     715      35

  Fragments arranged by size: 

                 715     378      35
 
BsmI GAATG_Cn'

Cuts at:      0     527    1006    1128 
   Size:        527     479     122

  Fragments arranged by size: 

                 527     479     122
 
Bsp1286I G_dGCh'C

Cuts at:      0     347     378    1093    1128 
   Size:        347      31     715      35

  Fragments arranged by size: 

                 715     347      35      31
 
BspHI T'CATG_A

Cuts at:      0     747    1128 
   Size:        747     381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     445    1128 
   Size:        445     683
 
BsrDI GCAATG_nn'

Cuts at:      0    1066    1128 
   Size:       1066      62
 
BsrGI T'GTAC_A

Cuts at:      0     253     977    1128 
   Size:        253     724     151

  Fragments arranged by size: 

                 724     253     151
 
BssSI C'ACGA_G

Cuts at:      0    1106    1128 
   Size:       1106      22
 
Bsu36I CC'TnA_GG

Cuts at:      0    1028    1128 
   Size:       1028     100
 
DraIII CAC_nnn'GTG

Cuts at:      0     382    1128 
   Size:        382     746
 
EarI CTCTTCn'nnn_

Cuts at:      0     189     773    1128 
   Size:        189     584     355

  Fragments arranged by size: 

                 584     355     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     523    1128 
   Size:         44     479     605

  Fragments arranged by size: 

                 605     479      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     899    1128 
   Size:        899     229
 
EcoRV GAT'ATC

Cuts at:      0     155    1128 
   Size:        155     973
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     688    1128 
   Size:        688     440
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     748     780    1128 
   Size:        748      32     348

  Fragments arranged by size: 

                 748     348      32
 
HindIII A'AGCT_T

Cuts at:      0     581    1128 
   Size:        581     547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     366    1128 
   Size:        366     762
 
MslI CAynn'nnrTG

Cuts at:      0     399    1128 
   Size:        399     729
 
MspA1I CmG'CkG

Cuts at:      0      33      54      64    1128 
   Size:         33      21      10    1064

  Fragments arranged by size: 

                1064      33      21      10
 
NspI r_CATG'y

Cuts at:      0     922    1064    1128 
   Size:        922     142      64

  Fragments arranged by size: 

                 922     142      64
 
PasI CC'CwG_GG

Cuts at:      0     288    1128 
   Size:        288     840
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     385    1128 
   Size:        385     743
 
ScaI AGT'ACT

Cuts at:      0    1010    1128 
   Size:       1010     118
 
SexAI A'CCwGG_T

Cuts at:      0     378    1128 
   Size:        378     750
 
SmlI C'TyrA_G

Cuts at:      0     271    1086    1128 
   Size:        271     815      42

  Fragments arranged by size: 

                 815     271      42
 
SpeI A'CTAG_T

Cuts at:      0     711    1128 
   Size:        711     417
 
SspI AAT'ATT

Cuts at:      0     609    1128 
   Size:        609     519
 
StuI AGG'CCT

Cuts at:      0     883    1128 
   Size:        883     245
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     968    1002    1128 
   Size:        968      34     126

  Fragments arranged by size: 

                 968     126      34
 
TatI w'GTAC_w

Cuts at:      0     253     977    1008    1128 
   Size:        253     724      31     120

  Fragments arranged by size: 

                 724     253     120      31
 
XmnI GAAnn'nnTTC

Cuts at:      0      13    1128 
   Size:         13    1115
 

 Enzymes that do cut:

     AarI     AccI     AcuI    ApaLI     ApoI     BaeI     BbsI    BbvCI
     BclI     BlpI Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BsrGI    BssSI   Bsu36I   DraIII     EarI  Eco57MI
    EcoNI    EcoRV     FalI    Hin4I  HindIII     MmeI     MslI   MspA1I
     NspI     PasI    PflMI     ScaI    SexAI     SmlI     SpeI     SspI
     StuI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI    BsaAI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI