Restriction map for
pF1KB6579
(Linear) (Six-base) MAPSORT of: KIBB6579.seq Check: 9157 from: 1 to: 1131
KIBB6579.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1131
Size: 226 905
AfeI AGC'GCT
Cuts at: 0 766 1131
Size: 766 365
AvaI C'yCGr_G
Cuts at: 0 298 1131
Size: 298 833
AvrII C'CTAG_G
Cuts at: 0 85 1131
Size: 85 1046
BanI G'GyrC_C
Cuts at: 0 263 910 966 1131
Size: 263 647 56 165
Fragments arranged by size:
647 263 165 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 1131
Size: 17 1114
BciVI GTATCCnnnnn_n'
Cuts at: 0 205 1131
Size: 205 926
BglI GCCn_nnn'nGGC
Cuts at: 0 88 415 738 1131
Size: 88 327 323 393
Fragments arranged by size:
393 327 323 88
BglII A'GATC_T
Cuts at: 0 257 1131
Size: 257 874
BlpI GC'TnA_GC
Cuts at: 0 617 1131
Size: 617 514
Bme1580I G_kGCm'C
Cuts at: 0 395 497 913 1131
Size: 395 102 416 218
Fragments arranged by size:
416 395 218 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 1131
Size: 249 882
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 373 826 1131
Size: 10 363 453 305
Fragments arranged by size:
453 363 305 10
BsaAI yAC'GTr
Cuts at: 0 165 405 660 1131
Size: 165 240 255 471
Fragments arranged by size:
471 255 240 165
BsaBI GATnn'nnATC
Cuts at: 0 577 988 1072 1131
Size: 577 411 84 59
Fragments arranged by size:
577 411 84 59
BsaHI Gr'CG_yC
Cuts at: 0 80 131 479 1131
Size: 80 51 348 652
Fragments arranged by size:
652 348 80 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 637 667 1131
Size: 28 30 579 30 464
Fragments arranged by size:
579 464 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 690 693 696 1131
Size: 316 374 3 3 435
Fragments arranged by size:
435 374 316 3 3
BseYI C'CCAG_C
Cuts at: 0 970 1131
Size: 970 161
BsiEI CG_ry'CG
Cuts at: 0 695 1131
Size: 695 436
BsiHKAI G_wGCw'C
Cuts at: 0 224 308 1131
Size: 224 84 823
Fragments arranged by size:
823 224 84
BsmI GAATG_Cn'
Cuts at: 0 824 1131
Size: 824 307
BsmBI CGTCTCn'nnnn_
Cuts at: 0 775 1131
Size: 775 356
Bsp1286I G_dGCh'C
Cuts at: 0 224 308 395 497 913 1131
Size: 224 84 87 102 416 218
Fragments arranged by size:
416 224 218 102 87 84
BspHI T'CATG_A
Cuts at: 0 851 1131
Size: 851 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 732 1131
Size: 732 399
BsrFI r'CCGG_y
Cuts at: 0 61 451 1099 1131
Size: 61 390 648 32
Fragments arranged by size:
648 390 61 32
BssSI C'ACGA_G
Cuts at: 0 28 829 1131
Size: 28 801 302
Fragments arranged by size:
801 302 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1131
Size: 405 726
BstYI r'GATC_y
Cuts at: 0 257 578 1131
Size: 257 321 553
Fragments arranged by size:
553 321 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 978 1131
Size: 978 153
DraIII CAC_nnn'GTG
Cuts at: 0 492 1131
Size: 492 639
DrdI GACnn_nn'nnGTC
Cuts at: 0 742 1131
Size: 742 389
EaeI y'GGCC_r
Cuts at: 0 407 692 1131
Size: 407 285 439
Fragments arranged by size:
439 407 285
EagI C'GGCC_G
Cuts at: 0 692 1131
Size: 692 439
EarI CTCTTCn'nnn_
Cuts at: 0 793 1131
Size: 793 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1131
Size: 226 905
EcoNI CCTnn'n_nnAGG
Cuts at: 0 441 1131
Size: 441 690
EcoO109I rG'GnC_Cy
Cuts at: 0 327 879 1131
Size: 327 552 252
Fragments arranged by size:
552 327 252
FseI GG_CCGG'CC
Cuts at: 0 1103 1131
Size: 1103 28
HaeII r_GCGC'y
Cuts at: 0 768 1131
Size: 768 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 1131
Size: 28 32 1071
Fragments arranged by size:
1071 32 28
MscI TGG'CCA
Cuts at: 0 409 1131
Size: 409 722
MslI CAynn'nnrTG
Cuts at: 0 403 808 916 1131
Size: 403 405 108 215
Fragments arranged by size:
405 403 215 108
MspA1I CmG'CkG
Cuts at: 0 616 773 1131
Size: 616 157 358
Fragments arranged by size:
616 358 157
NaeI GCC'GGC
Cuts at: 0 63 1101 1131
Size: 63 1038 30
Fragments arranged by size:
1038 63 30
NgoMIV G'CCGG_C
Cuts at: 0 61 1099 1131
Size: 61 1038 32
Fragments arranged by size:
1038 61 32
NspI r_CATG'y
Cuts at: 0 945 1131
Size: 945 186
PasI CC'CwG_GG
Cuts at: 0 925 1131
Size: 925 206
PpuMI rG'GwC_Cy
Cuts at: 0 879 1131
Size: 879 252
PvuII CAG'CTG
Cuts at: 0 616 1131
Size: 616 515
SapI GCTCTTCn'nnn_
Cuts at: 0 793 1131
Size: 793 338
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 415 1131
Size: 415 716
SphI G_CATG'C
Cuts at: 0 945 1131
Size: 945 186
StyI C'CwwG_G
Cuts at: 0 85 341 1131
Size: 85 256 790
Fragments arranged by size:
790 256 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 984 1131
Size: 499 485 147
Fragments arranged by size:
499 485 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 1131
Size: 637 32 462
Fragments arranged by size:
637 462 32
Tth111I GACn'n_nGTC
Cuts at: 0 478 1131
Size: 478 653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 1131
Size: 534 597
Enzymes that do cut:
AcuI AfeI AvaI AvrII BanI BbsI BciVI BglI
BglII BlpI Bme1580I BmrI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BssSI BstXI BstYI BtgZI DraIII
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV NspI
PasI PpuMI PvuII SapI SfiI SphI StyI TaqII
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BanII BbeI BbvCI
BcgI BclI BfrBI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaWI BsgI BsiWI BspEI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI EciI EcoICRI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TatI XbaI XhoI XmaI XmnI
ZraI