(Linear) (Six-base) MAPSORT of: KIBB6580.seq  Check: 2000  from: 1  to: 1209



KIBB6580.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     134    1209 
   Size:        134    1075
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     618     650     694     726    1209 
   Size:        618      32      44      32     483

  Fragments arranged by size: 

                 618     483      44      32      32
 
ApoI r'AATT_y

Cuts at:      0     238     430    1017    1209 
   Size:        238     192     587     192

  Fragments arranged by size: 

                 587     238     192     192
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     278     710    1209 
   Size:        278     432     499

  Fragments arranged by size: 

                 499     432     278
 
BbvCI CC'TCA_GC

Cuts at:      0     731    1209 
   Size:        731     478
 
BclI T'GATC_A

Cuts at:      0     888    1122    1209 
   Size:        888     234      87

  Fragments arranged by size: 

                 888     234      87
 
BglII A'GATC_T

Cuts at:      0     318    1209 
   Size:        318     891
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     500    1209 
   Size:        500     709
 
BmtI G_CTAG'C

Cuts at:      0    1079    1209 
   Size:       1079     130
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     873    1209 
   Size:        873     336
 
Bpu10I CC'TnA_GC

Cuts at:      0     731    1209 
   Size:        731     478
 
BsaBI GATnn'nnATC

Cuts at:      0     347    1181    1209 
   Size:        347     834      28

  Fragments arranged by size: 

                 834     347      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1069    1209 
   Size:       1069     140
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     944    1209 
   Size:        944     265
 
BstYI r'GATC_y

Cuts at:      0     318    1209 
   Size:        318     891
 
BstZ17I GTA'TAC

Cuts at:      0     135    1209 
   Size:        135    1074
 
ClaI AT'CG_AT

Cuts at:      0     899    1209 
   Size:        899     310
 
DraI TTT'AAA

Cuts at:      0     437    1209 
   Size:        437     772
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     638    1209 
   Size:        638     571
 
EcoO109I rG'GnC_Cy

Cuts at:      0     450     688    1209 
   Size:        450     238     521

  Fragments arranged by size: 

                 521     450     238
 
EcoRV GAT'ATC

Cuts at:      0      46     756    1209 
   Size:         46     710     453

  Fragments arranged by size: 

                 710     453      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1053    1209 
   Size:       1053     156
 
HaeII r_GCGC'y

Cuts at:      0     519    1209 
   Size:        519     690
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     189     221    1045    1077    1209 
   Size:        189      32     824      32     132

  Fragments arranged by size: 

                 824     189     132      32      32
 
NheI G'CTAG_C

Cuts at:      0    1075    1209 
   Size:       1075     134
 
PpuMI rG'GwC_Cy

Cuts at:      0     688    1209 
   Size:        688     521
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     709     741    1209 
   Size:        709      32     468

  Fragments arranged by size: 

                 709     468      32
 
PstI C_TGCA'G

Cuts at:      0     117    1209 
   Size:        117    1092
 
ScaI AGT'ACT

Cuts at:      0     950    1209 
   Size:        950     259
 
SfcI C'TryA_G

Cuts at:      0     113    1209 
   Size:        113    1096
 
SspI AAT'ATT

Cuts at:      0     147    1209 
   Size:        147    1062
 
TatI w'GTAC_w

Cuts at:      0     716     948    1034    1209 
   Size:        716     232      86     175

  Fragments arranged by size: 

                 716     232     175      86
 
XmnI GAAnn'nnTTC

Cuts at:      0     605    1209 
   Size:        605     604
 

 Enzymes that do cut:

     AccI     AloI     ApoI     BbsI    BbvCI     BclI    BglII     BmrI
     BmtI     BplI   Bpu10I    BsaBI    BseRI     BsgI    BstYI  BstZ17I
     ClaI     DraI     EciI EcoO109I    EcoRV     FalI    HaeII    Hin4I
     NheI    PpuMI     PsrI     PstI     ScaI     SfcI     SspI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI