Restriction map for
pF1KB6585
(Linear) (Six-base) MAPSORT of: KIBB6585.seq Check: 7061 from: 1 to: 1131
KIBB6585.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 0 117 1131
Size: 117 1014
AclI AA'CG_TT
Cuts at: 0 765 1131
Size: 765 366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 320 1131
Size: 226 94 811
Fragments arranged by size:
811 226 94
BanI G'GyrC_C
Cuts at: 0 263 327 910 1131
Size: 263 64 583 221
Fragments arranged by size:
583 263 221 64
BanII G_rGCy'C
Cuts at: 0 53 1131
Size: 53 1078
BclI T'GATC_A
Cuts at: 0 746 1131
Size: 746 385
BglI GCCn_nnn'nGGC
Cuts at: 0 738 1131
Size: 738 393
BglII A'GATC_T
Cuts at: 0 257 555 1131
Size: 257 298 576
Fragments arranged by size:
576 298 257
BlpI GC'TnA_GC
Cuts at: 0 617 1131
Size: 617 514
Bme1580I G_kGCm'C
Cuts at: 0 913 1131
Size: 913 218
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 1131
Size: 249 882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1131
Size: 491 640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1131
Size: 733 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 1131
Size: 775 356
BsaAI yAC'GTr
Cuts at: 0 165 1131
Size: 165 966
BsaBI GATnn'nnATC
Cuts at: 0 751 988 1072 1131
Size: 751 237 84 59
Fragments arranged by size:
751 237 84 59
BsaHI Gr'CG_yC
Cuts at: 0 114 1131
Size: 114 1017
BsaWI w'CCGG_w
Cuts at: 0 1002 1131
Size: 1002 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 464 494 637 667 1131
Size: 464 30 143 30 464
Fragments arranged by size:
464 464 143 30 30
BseYI C'CCAG_C
Cuts at: 0 70 393 970 1131
Size: 70 323 577 161
Fragments arranged by size:
577 323 161 70
BsiEI CG_ry'CG
Cuts at: 0 939 1131
Size: 939 192
BsmI GAATG_Cn'
Cuts at: 0 947 1131
Size: 947 184
Bsp1286I G_dGCh'C
Cuts at: 0 53 913 1131
Size: 53 860 218
Fragments arranged by size:
860 218 53
BspEI T'CCGG_A
Cuts at: 0 1002 1131
Size: 1002 129
BspHI T'CATG_A
Cuts at: 0 572 851 1131
Size: 572 279 280
Fragments arranged by size:
572 280 279
BsrDI GCAATG_nn'
Cuts at: 0 929 992 1131
Size: 929 63 139
Fragments arranged by size:
929 139 63
BsrFI r'CCGG_y
Cuts at: 0 61 1131
Size: 61 1070
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1131
Size: 405 726
BstYI r'GATC_y
Cuts at: 0 257 540 555 578 1131
Size: 257 283 15 23 553
Fragments arranged by size:
553 283 257 23 15
BtsI GCAGTG_nn'
Cuts at: 0 20 689 1131
Size: 20 669 442
Fragments arranged by size:
669 442 20
EaeI y'GGCC_r
Cuts at: 0 549 686 1131
Size: 549 137 445
Fragments arranged by size:
549 445 137
EarI CTCTTCn'nnn_
Cuts at: 0 5 296 712 1131
Size: 5 291 416 419
Fragments arranged by size:
419 416 291 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 320 491 1131
Size: 226 94 171 640
Fragments arranged by size:
640 226 171 94
EcoO109I rG'GnC_Cy
Cuts at: 0 879 1131
Size: 879 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 1131
Size: 81 1050
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 464 496 1131
Size: 28 32 404 32 635
Fragments arranged by size:
635 404 32 32 28
MscI TGG'CCA
Cuts at: 0 688 1131
Size: 688 443
MslI CAynn'nnrTG
Cuts at: 0 403 808 916 1131
Size: 403 405 108 215
Fragments arranged by size:
405 403 215 108
MspA1I CmG'CkG
Cuts at: 0 773 1131
Size: 773 358
NaeI GCC'GGC
Cuts at: 0 63 1131
Size: 63 1068
NgoMIV G'CCGG_C
Cuts at: 0 61 1131
Size: 61 1070
PasI CC'CwG_GG
Cuts at: 0 86 1131
Size: 86 1045
PfoI T'CCnGG_A
Cuts at: 0 632 1131
Size: 632 499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1131
Size: 772 32 327
Fragments arranged by size:
772 327 32
PpuMI rG'GwC_Cy
Cuts at: 0 879 1131
Size: 879 252
SapI GCTCTTCn'nnn_
Cuts at: 0 296 1131
Size: 296 835
SmlI C'TyrA_G
Cuts at: 0 712 1131
Size: 712 419
StyI C'CwwG_G
Cuts at: 0 26 341 1131
Size: 26 315 790
Fragments arranged by size:
790 315 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 499 953 984 1131
Size: 157 342 454 31 147
Fragments arranged by size:
454 342 157 147 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 1131
Size: 637 32 462
Fragments arranged by size:
637 462 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 1131
Size: 534 597
ZraI GAC'GTC
Cuts at: 0 115 1131
Size: 115 1016
Enzymes that do cut:
AatII AclI AcuI BanI BanII BclI BglI BglII
BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsmI Bsp1286I BspEI
BspHI BsrDI BsrFI BstXI BstYI BtsI EaeI EarI
Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I NaeI
NgoMIV PasI PfoI PpiI PpuMI SapI SmlI StyI
TaqII TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI Bpu10I
BseRI BsgI BsiHKAI BsiWI BsmBI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI