Restriction map for
pF1KB6586
(Linear) (Six-base) MAPSORT of: KIBB6586.seq Check: 1057 from: 1 to: 1218
KIBB6586.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 819 1218
Size: 819 399
AccI GT'mk_AC
Cuts at: 0 416 1218
Size: 416 802
Acc65I G'GTAC_C
Cuts at: 0 696 1218
Size: 696 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1218
Size: 328 890
AflII C'TTAA_G
Cuts at: 0 562 871 1218
Size: 562 309 347
Fragments arranged by size:
562 347 309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 1218
Size: 280 938
AlwNI CAG_nnn'CTG
Cuts at: 0 273 1218
Size: 273 945
ApaLI G'TGCA_C
Cuts at: 0 1042 1218
Size: 1042 176
ApoI r'AATT_y
Cuts at: 0 913 1218
Size: 913 305
AvaI C'yCGr_G
Cuts at: 0 71 895 1218
Size: 71 824 323
Fragments arranged by size:
824 323 71
BanI G'GyrC_C
Cuts at: 0 109 649 696 1218
Size: 109 540 47 522
Fragments arranged by size:
540 522 109 47
BanII G_rGCy'C
Cuts at: 0 931 1218
Size: 931 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 454 969 1218
Size: 454 515 249
Fragments arranged by size:
515 454 249
BbvCI CC'TCA_GC
Cuts at: 0 859 1218
Size: 859 359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 567 596 601 1218
Size: 562 5 29 5 617
Fragments arranged by size:
617 562 29 5 5
BclI T'GATC_A
Cuts at: 0 590 1218
Size: 590 628
BglI GCCn_nnn'nGGC
Cuts at: 0 118 1218
Size: 118 1100
BlpI GC'TnA_GC
Cuts at: 0 379 1218
Size: 379 839
Bme1580I G_kGCm'C
Cuts at: 0 1046 1218
Size: 1046 172
BmrI ACTGGGnnnn_n'
Cuts at: 0 803 950 1218
Size: 803 147 268
Fragments arranged by size:
803 268 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 295 961 1218
Size: 280 15 666 257
Fragments arranged by size:
666 280 257 15
Bpu10I CC'TnA_GC
Cuts at: 0 859 1218
Size: 859 359
BsaAI yAC'GTr
Cuts at: 0 672 1218
Size: 672 546
BsaBI GATnn'nnATC
Cuts at: 0 268 722 1218
Size: 268 454 496
Fragments arranged by size:
496 454 268
BsaWI w'CCGG_w
Cuts at: 0 740 1218
Size: 740 478
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 1218
Size: 4 1214
BseYI C'CCAG_C
Cuts at: 0 124 181 247 1218
Size: 124 57 66 971
Fragments arranged by size:
971 124 66 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1218
Size: 304 914
BsiEI CG_ry'CG
Cuts at: 0 587 1218
Size: 587 631
BsiHKAI G_wGCw'C
Cuts at: 0 1046 1218
Size: 1046 172
Bsp1286I G_dGCh'C
Cuts at: 0 931 1046 1218
Size: 931 115 172
Fragments arranged by size:
931 172 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 819 1218
Size: 428 391 399
Fragments arranged by size:
428 399 391
BsrDI GCAATG_nn'
Cuts at: 0 396 585 789 1218
Size: 396 189 204 429
Fragments arranged by size:
429 396 204 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 1218
Size: 225 993
BstYI r'GATC_y
Cuts at: 0 269 1218
Size: 269 949
Bsu36I CC'TnA_GG
Cuts at: 0 41 1218
Size: 41 1177
BtgI C'CryG_G
Cuts at: 0 113 788 1094 1218
Size: 113 675 306 124
Fragments arranged by size:
675 306 124 113
DraI TTT'AAA
Cuts at: 0 615 1218
Size: 615 603
EaeI y'GGCC_r
Cuts at: 0 227 1218
Size: 227 991
EarI CTCTTCn'nnn_
Cuts at: 0 13 79 1198 1218
Size: 13 66 1119 20
Fragments arranged by size:
1119 66 20 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 295 328 961 1218
Size: 280 15 33 633 257
Fragments arranged by size:
633 280 257 33 15
EcoO109I rG'GnC_Cy
Cuts at: 0 47 96 161 279 768 1157 1218
Size: 47 49 65 118 489 389 61
Fragments arranged by size:
489 389 118 65 61 49 47
HindIII A'AGCT_T
Cuts at: 0 633 1218
Size: 633 585
KpnI G_GTAC'C
Cuts at: 0 700 1218
Size: 700 518
MscI TGG'CCA
Cuts at: 0 229 1218
Size: 229 989
MslI CAynn'nnrTG
Cuts at: 0 107 217 262 1218
Size: 107 110 45 956
Fragments arranged by size:
956 110 107 45
MspA1I CmG'CkG
Cuts at: 0 175 378 939 1087 1218
Size: 175 203 561 148 131
Fragments arranged by size:
561 203 175 148 131
PasI CC'CwG_GG
Cuts at: 0 240 1218
Size: 240 978
PfoI T'CCnGG_A
Cuts at: 0 272 734 1218
Size: 272 462 484
Fragments arranged by size:
484 462 272
PpuMI rG'GwC_Cy
Cuts at: 0 96 768 1218
Size: 96 672 450
Fragments arranged by size:
672 450 96
PspXI vC'TCGA_Gb
Cuts at: 0 895 1218
Size: 895 323
PstI C_TGCA'G
Cuts at: 0 425 498 757 1218
Size: 425 73 259 461
Fragments arranged by size:
461 425 259 73
PvuII CAG'CTG
Cuts at: 0 378 939 1218
Size: 378 561 279
Fragments arranged by size:
561 378 279
SanDI GG'GwC_CC
Cuts at: 0 768 1218
Size: 768 450
SapI GCTCTTCn'nnn_
Cuts at: 0 13 1218
Size: 13 1205
SbfI CC_TGCA'GG
Cuts at: 0 425 757 1218
Size: 425 332 461
Fragments arranged by size:
461 425 332
SexAI A'CCwGG_T
Cuts at: 0 407 1218
Size: 407 811
SfcI C'TryA_G
Cuts at: 0 156 421 494 753 1218
Size: 156 265 73 259 465
Fragments arranged by size:
465 265 259 156 73
SmaI CCC'GGG
Cuts at: 0 73 1218
Size: 73 1145
SmlI C'TyrA_G
Cuts at: 0 562 871 895 1218
Size: 562 309 24 323
Fragments arranged by size:
562 323 309 24
SspI AAT'ATT
Cuts at: 0 1106 1218
Size: 1106 112
StyI C'CwwG_G
Cuts at: 0 164 777 1197 1218
Size: 164 613 420 21
Fragments arranged by size:
613 420 164 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 1218
Size: 667 551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 364 1218
Size: 237 127 854
Fragments arranged by size:
854 237 127
XhoI C'TCGA_G
Cuts at: 0 895 1218
Size: 895 323
XmaI C'CCGG_G
Cuts at: 0 71 1218
Size: 71 1147
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrDI BstXI BstYI Bsu36I BtgI DraI EaeI EarI
Eco57MI EcoO109I HindIII KpnI MscI MslI MspA1I PasI
PfoI PpuMI PspXI PstI PvuII SanDI SapI SbfI
SexAI SfcI SmaI SmlI SspI StyI TaqII XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BfrBI BglII BmgBI BmtI BplI BpuEI BsaI
BsaHI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI Tth111I XbaI
XmnI ZraI