Restriction map for
pF1KB6590
(Linear) (Six-base) MAPSORT of: KIBB6590.seq Check: 7414 from: 1 to: 1218
KIBB6590.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 0 668 1218
Size: 668 550
AccI GT'mk_AC
Cuts at: 0 872 1218
Size: 872 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1218
Size: 157 1061
AgeI A'CCGG_T
Cuts at: 0 877 1218
Size: 877 341
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 422 454 1218
Size: 422 32 764
Fragments arranged by size:
764 422 32
ApaI G_GGCC'C
Cuts at: 0 985 1140 1218
Size: 985 155 78
Fragments arranged by size:
985 155 78
ApaLI G'TGCA_C
Cuts at: 0 1083 1218
Size: 1083 135
ApoI r'AATT_y
Cuts at: 0 699 762 1218
Size: 699 63 456
Fragments arranged by size:
699 456 63
AvaI C'yCGr_G
Cuts at: 0 172 1218
Size: 172 1046
BanI G'GyrC_C
Cuts at: 0 277 333 1218
Size: 277 56 885
Fragments arranged by size:
885 277 56
BanII G_rGCy'C
Cuts at: 0 55 985 1140 1218
Size: 55 930 155 78
Fragments arranged by size:
930 155 78 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 857 1218
Size: 660 197 361
Fragments arranged by size:
660 361 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1185 1218
Size: 1185 33
BglI GCCn_nnn'nGGC
Cuts at: 0 1144 1218
Size: 1144 74
Bme1580I G_kGCm'C
Cuts at: 0 338 985 1087 1140 1218
Size: 338 647 102 53 78
Fragments arranged by size:
647 338 102 78 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 246 1218
Size: 246 972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1218
Size: 390 828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1218
Size: 91 1127
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 1218
Size: 73 1145
BsaBI GATnn'nnATC
Cuts at: 0 271 1218
Size: 271 947
BsaHI Gr'CG_yC
Cuts at: 0 665 1218
Size: 665 553
BsaWI w'CCGG_w
Cuts at: 0 877 1218
Size: 877 341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 422 452 625 655 1218
Size: 202 30 190 30 173 30 563
Fragments arranged by size:
563 202 190 173 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 330 1218
Size: 210 120 888
Fragments arranged by size:
888 210 120
BseYI C'CCAG_C
Cuts at: 0 26 711 729 746 1218
Size: 26 685 18 17 472
Fragments arranged by size:
685 472 26 18 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1218
Size: 369 849
BsiHKAI G_wGCw'C
Cuts at: 0 1087 1218
Size: 1087 131
BsmI GAATG_Cn'
Cuts at: 0 119 1218
Size: 119 1099
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 1218
Size: 361 857
Bsp1286I G_dGCh'C
Cuts at: 0 55 338 985 1087 1140 1218
Size: 55 283 647 102 53 78
Fragments arranged by size:
647 283 102 78 55 53
BsrFI r'CCGG_y
Cuts at: 0 877 1218
Size: 877 341
BstEII G'GTnAC_C
Cuts at: 0 615 1218
Size: 615 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 259 1218
Size: 243 16 959
Fragments arranged by size:
959 243 16
BstYI r'GATC_y
Cuts at: 0 98 1218
Size: 98 1120
BtgI C'CryG_G
Cuts at: 0 491 1103 1218
Size: 491 612 115
Fragments arranged by size:
612 491 115
BtsI GCAGTG_nn'
Cuts at: 0 362 1218
Size: 362 856
DraI TTT'AAA
Cuts at: 0 93 1218
Size: 93 1125
EaeI y'GGCC_r
Cuts at: 0 158 560 1091 1218
Size: 158 402 531 127
Fragments arranged by size:
531 402 158 127
EarI CTCTTCn'nnn_
Cuts at: 0 187 391 940 1218
Size: 187 204 549 278
Fragments arranged by size:
549 278 204 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 390 1218
Size: 157 233 828
Fragments arranged by size:
828 233 157
EcoO109I rG'GnC_Cy
Cuts at: 0 981 982 1137 1218
Size: 981 1 155 81
Fragments arranged by size:
981 155 81 1
EcoRI G'AATT_C
Cuts at: 0 699 1218
Size: 699 519
EcoRV GAT'ATC
Cuts at: 0 1110 1218
Size: 1110 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 1218
Size: 655 32 531
Fragments arranged by size:
655 531 32
MscI TGG'CCA
Cuts at: 0 160 1093 1218
Size: 160 933 125
Fragments arranged by size:
933 160 125
MslI CAynn'nnrTG
Cuts at: 0 490 535 1218
Size: 490 45 683
Fragments arranged by size:
683 490 45
NcoI C'CATG_G
Cuts at: 0 1103 1218
Size: 1103 115
PasI CC'CwG_GG
Cuts at: 0 1140 1218
Size: 1140 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 329 711 1104 1123 1218
Size: 329 382 393 19 95
Fragments arranged by size:
393 382 329 95 19
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 422 454 1218
Size: 422 32 764
Fragments arranged by size:
764 422 32
PshAI GACnn'nnGTC
Cuts at: 0 793 1218
Size: 793 425
PspOMI G'GGCC_C
Cuts at: 0 981 1136 1218
Size: 981 155 82
Fragments arranged by size:
981 155 82
SapI GCTCTTCn'nnn_
Cuts at: 0 391 1218
Size: 391 827
ScaI AGT'ACT
Cuts at: 0 256 1218
Size: 256 962
SfcI C'TryA_G
Cuts at: 0 1065 1218
Size: 1065 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1144 1218
Size: 1144 74
SgrAI Cr'CCGG_yG
Cuts at: 0 877 1218
Size: 877 341
SmlI C'TyrA_G
Cuts at: 0 106 1218
Size: 106 1112
StuI AGG'CCT
Cuts at: 0 931 1218
Size: 931 287
StyI C'CwwG_G
Cuts at: 0 645 932 1103 1208 1218
Size: 645 287 171 105 10
Fragments arranged by size:
645 287 171 105 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 321 484 1134 1218
Size: 265 56 163 650 84
Fragments arranged by size:
650 265 163 84 56
TatI w'GTAC_w
Cuts at: 0 254 1218
Size: 254 964
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 491 523 710 742 1079 1111
Size: 447 32 12 32 187 32 337 32
Cuts at: 1111 1218
Size: 107
Fragments arranged by size:
447 337 187 107 32 32 32 32
12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1101 1137 1218
Size: 1101 36 81
Fragments arranged by size:
1101 81 36
ZraI GAC'GTC
Cuts at: 0 666 1218
Size: 666 552
Enzymes that do cut:
AatII AccI AcuI AgeI AloI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BcgI BglI Bme1580I BmrI
BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrFI BstEII
BstXI BstYI BtgI BtsI DraI EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV Hin4I MscI MslI NcoI PasI
PflMI PpiI PshAI PspOMI SapI ScaI SfcI SfiI
SgrAI SmlI StuI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaAI BsiEI BsiWI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI XmnI