Restriction map for
pF1KB6592
(Linear) (Six-base) MAPSORT of: KIBB6592.seq Check: 4452 from: 1 to: 1218
KIBB6592.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1218
Size: 505 713
AflIII A'CryG_T
Cuts at: 0 576 1218
Size: 576 642
AleI CACnn'nnGTG
Cuts at: 0 656 1218
Size: 656 562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 791 1218
Size: 791 427
AlwNI CAG_nnn'CTG
Cuts at: 0 87 195 1218
Size: 87 108 1023
Fragments arranged by size:
1023 108 87
ApoI r'AATT_y
Cuts at: 0 39 386 598 838 1218
Size: 39 347 212 240 380
Fragments arranged by size:
380 347 240 212 39
BamHI G'GATC_C
Cuts at: 0 785 1218
Size: 785 433
BanI G'GyrC_C
Cuts at: 0 761 1218
Size: 761 457
BanII G_rGCy'C
Cuts at: 0 632 978 1218
Size: 632 346 240
Fragments arranged by size:
632 346 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 709 743 1218
Size: 709 34 475
Fragments arranged by size:
709 475 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1210 1218
Size: 1210 8
BglII A'GATC_T
Cuts at: 0 60 1218
Size: 60 1158
BmtI G_CTAG'C
Cuts at: 0 739 1218
Size: 739 479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 68 1218
Size: 68 1150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 454 487 1218
Size: 131 323 33 731
Fragments arranged by size:
731 323 131 33
Bpu10I CC'TnA_GC
Cuts at: 0 998 1218
Size: 998 220
BsaAI yAC'GTr
Cuts at: 0 577 1218
Size: 577 641
BsaBI GATnn'nnATC
Cuts at: 0 476 1218
Size: 476 742
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 1218
Size: 313 905
BseYI C'CCAG_C
Cuts at: 0 29 1218
Size: 29 1189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1218
Size: 596 622
BsiHKAI G_wGCw'C
Cuts at: 0 632 978 1218
Size: 632 346 240
Fragments arranged by size:
632 346 240
Bsp1286I G_dGCh'C
Cuts at: 0 632 978 1218
Size: 632 346 240
Fragments arranged by size:
632 346 240
BspHI T'CATG_A
Cuts at: 0 35 1218
Size: 35 1183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 1218
Size: 294 924
BsrBI CCG'CTC
Cuts at: 0 47 1218
Size: 47 1171
BsrDI GCAATG_nn'
Cuts at: 0 158 1218
Size: 158 1060
BssSI C'ACGA_G
Cuts at: 0 175 1088 1218
Size: 175 913 130
Fragments arranged by size:
913 175 130
BstBI TT'CG_AA
Cuts at: 0 962 1218
Size: 962 256
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 1066 1218
Size: 897 169 152
Fragments arranged by size:
897 169 152
BstYI r'GATC_y
Cuts at: 0 60 83 272 785 986 1118 1218
Size: 60 23 189 513 201 132 100
Fragments arranged by size:
513 201 189 132 100 60 23
BtgI C'CryG_G
Cuts at: 0 1196 1218
Size: 1196 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1018 1218
Size: 1018 200
EcoICRI GAG'CTC
Cuts at: 0 630 976 1218
Size: 630 346 242
Fragments arranged by size:
630 346 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 454 487 505 1218
Size: 131 323 33 18 713
Fragments arranged by size:
713 323 131 33 18
EcoRI G'AATT_C
Cuts at: 0 39 386 1218
Size: 39 347 832
Fragments arranged by size:
832 347 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1002 1034 1218
Size: 1002 32 184
Fragments arranged by size:
1002 184 32
HindIII A'AGCT_T
Cuts at: 0 128 137 1218
Size: 128 9 1081
Fragments arranged by size:
1081 128 9
MslI CAynn'nnrTG
Cuts at: 0 656 853 1179 1218
Size: 656 197 326 39
Fragments arranged by size:
656 326 197 39
MspA1I CmG'CkG
Cuts at: 0 880 1218
Size: 880 338
NcoI C'CATG_G
Cuts at: 0 1196 1218
Size: 1196 22
NheI G'CTAG_C
Cuts at: 0 735 1218
Size: 735 483
NspI r_CATG'y
Cuts at: 0 685 1218
Size: 685 533
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 1218
Size: 378 840
PfoI T'CCnGG_A
Cuts at: 0 86 1218
Size: 86 1132
PmlI CAC'GTG
Cuts at: 0 577 1218
Size: 577 641
PshAI GACnn'nnGTC
Cuts at: 0 646 1218
Size: 646 572
PsiI TTA'TAA
Cuts at: 0 428 560 1218
Size: 428 132 658
Fragments arranged by size:
658 428 132
PstI C_TGCA'G
Cuts at: 0 996 1218
Size: 996 222
PvuII CAG'CTG
Cuts at: 0 880 1218
Size: 880 338
SacI G_AGCT'C
Cuts at: 0 632 978 1218
Size: 632 346 240
Fragments arranged by size:
632 346 240
SfcI C'TryA_G
Cuts at: 0 875 992 1218
Size: 875 117 226
Fragments arranged by size:
875 226 117
SspI AAT'ATT
Cuts at: 0 954 1218
Size: 954 264
StuI AGG'CCT
Cuts at: 0 673 1218
Size: 673 545
StyI C'CwwG_G
Cuts at: 0 263 1196 1218
Size: 263 933 22
Fragments arranged by size:
933 263 22
TatI w'GTAC_w
Cuts at: 0 413 1218
Size: 413 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 745 777 1118 1150 1218
Size: 201 32 512 32 341 32 68
Fragments arranged by size:
512 341 201 68 32 32 32
XbaI T'CTAG_A
Cuts at: 0 804 1218
Size: 804 414
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApoI BamHI BanI
BanII BcgI BglI BglII BmtI BplI BpmI Bpu10I
BsaAI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BssSI BstBI BstXI BstYI BtgI
EciI EcoICRI Eco57MI EcoRI Hin4I HindIII MslI MspA1I
NcoI NheI NspI PflMI PfoI PmlI PshAI PsiI
PstI PvuII SacI SfcI SspI StuI StyI TatI
TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI
BlpI Bme1580I BmgBI BmrI BpuEI BsaI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI
BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PciI PmeI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI TaqII Tth111I XcmI XhoI XmaI
XmnI ZraI