Restriction map for
pF1KB6596
(Linear) (Six-base) MAPSORT of: KIBB6596.seq Check: 7338 from: 1 to: 1221
KIBB6596.orf
With 182 enzymes: SgfI *
November 7, 2014 16:44 ..
AccI GT'mk_AC
Cuts at: 0 857 1221
Size: 857 364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1179 1221
Size: 856 323 42
Fragments arranged by size:
856 323 42
AleI CACnn'nnGTG
Cuts at: 0 442 593 652 1081 1221
Size: 442 151 59 429 140
Fragments arranged by size:
442 429 151 140 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 737 1221
Size: 737 484
ApaI G_GGCC'C
Cuts at: 0 1092 1221
Size: 1092 129
ApaLI G'TGCA_C
Cuts at: 0 762 1221
Size: 762 459
AvaI C'yCGr_G
Cuts at: 0 685 1221
Size: 685 536
BanII G_rGCy'C
Cuts at: 0 889 1092 1221
Size: 889 203 129
Fragments arranged by size:
889 203 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 1221
Size: 339 882
BfrBI ATG'CAT
Cuts at: 0 643 1221
Size: 643 578
BglI GCCn_nnn'nGGC
Cuts at: 0 241 1221
Size: 241 980
BglII A'GATC_T
Cuts at: 0 825 1221
Size: 825 396
Bme1580I G_kGCm'C
Cuts at: 0 766 1092 1221
Size: 766 326 129
Fragments arranged by size:
766 326 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 1221
Size: 735 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1009 1081 1221
Size: 397 612 72 140
Fragments arranged by size:
612 397 140 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 1057 1221
Size: 508 549 164
Fragments arranged by size:
549 508 164
BsaBI GATnn'nnATC
Cuts at: 0 163 1221
Size: 163 1058
BsaWI w'CCGG_w
Cuts at: 0 959 1221
Size: 959 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 395 425 1132 1162 1221
Size: 395 30 707 30 59
Fragments arranged by size:
707 395 59 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 684 1111 1114 1194 1221
Size: 684 427 3 80 27
Fragments arranged by size:
684 427 80 27 3
BseYI C'CCAG_C
Cuts at: 0 523 1221
Size: 523 698
BsiHKAI G_wGCw'C
Cuts at: 0 766 889 1217 1221
Size: 766 123 328 4
Fragments arranged by size:
766 328 123 4
BsmI GAATG_Cn'
Cuts at: 0 247 1221
Size: 247 974
Bsp1286I G_dGCh'C
Cuts at: 0 766 889 1092 1217 1221
Size: 766 123 203 125 4
Fragments arranged by size:
766 203 125 123 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 843 1221
Size: 843 378
BsrFI r'CCGG_y
Cuts at: 0 982 1221
Size: 982 239
BsrGI T'GTAC_A
Cuts at: 0 896 1043 1221
Size: 896 147 178
Fragments arranged by size:
896 178 147
BssSI C'ACGA_G
Cuts at: 0 709 1093 1221
Size: 709 384 128
Fragments arranged by size:
709 384 128
BstEII G'GTnAC_C
Cuts at: 0 129 1221
Size: 129 1092
BstXI CCAn_nnnn'nTGG
Cuts at: 0 444 1083 1221
Size: 444 639 138
Fragments arranged by size:
639 444 138
BstYI r'GATC_y
Cuts at: 0 680 825 1221
Size: 680 145 396
Fragments arranged by size:
680 396 145
BtgI C'CryG_G
Cuts at: 0 239 1221
Size: 239 982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 321 1221
Size: 321 900
BtsI GCAGTG_nn'
Cuts at: 0 1044 1221
Size: 1044 177
DraIII CAC_nnn'GTG
Cuts at: 0 1168 1221
Size: 1168 53
EaeI y'GGCC_r
Cuts at: 0 395 550 863 878 1221
Size: 395 155 313 15 343
Fragments arranged by size:
395 343 313 155 15
EarI CTCTTCn'nnn_
Cuts at: 0 1164 1221
Size: 1164 57
EcoICRI GAG'CTC
Cuts at: 0 887 1221
Size: 887 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 856 1009 1081 1179 1221
Size: 397 459 153 72 98 42
Fragments arranged by size:
459 397 153 98 72 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 384 1221
Size: 384 837
EcoO109I rG'GnC_Cy
Cuts at: 0 465 1088 1221
Size: 465 623 133
Fragments arranged by size:
623 465 133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 972 1221
Size: 972 249
HaeII r_GCGC'y
Cuts at: 0 983 1221
Size: 983 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1221
Size: 1132 32 57
Fragments arranged by size:
1132 57 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1221
Size: 1175 46
MscI TGG'CCA
Cuts at: 0 397 552 865 880 1221
Size: 397 155 313 15 341
Fragments arranged by size:
397 341 313 155 15
MslI CAynn'nnrTG
Cuts at: 0 127 442 593 595 652 1081 1221
Size: 127 315 151 2 57 429 140
Fragments arranged by size:
429 315 151 140 127 57 2
MspA1I CmG'CkG
Cuts at: 0 109 343 1221
Size: 109 234 878
Fragments arranged by size:
878 234 109
NaeI GCC'GGC
Cuts at: 0 984 1221
Size: 984 237
NcoI C'CATG_G
Cuts at: 0 239 1221
Size: 239 982
NgoMIV G'CCGG_C
Cuts at: 0 982 1221
Size: 982 239
NsiI A_TGCA'T
Cuts at: 0 645 1221
Size: 645 576
NspI r_CATG'y
Cuts at: 0 955 1221
Size: 955 266
PasI CC'CwG_GG
Cuts at: 0 492 1032 1221
Size: 492 540 189
Fragments arranged by size:
540 492 189
PflMI CCAn_nnn'nTGG
Cuts at: 0 779 872 1221
Size: 779 93 349
Fragments arranged by size:
779 349 93
PpuMI rG'GwC_Cy
Cuts at: 0 465 1221
Size: 465 756
PspOMI G'GGCC_C
Cuts at: 0 1088 1221
Size: 1088 133
PvuII CAG'CTG
Cuts at: 0 109 1221
Size: 109 1112
SacI G_AGCT'C
Cuts at: 0 889 1221
Size: 889 332
SapI GCTCTTCn'nnn_
Cuts at: 0 1164 1221
Size: 1164 57
SgrAI Cr'CCGG_yG
Cuts at: 0 982 1221
Size: 982 239
SmaI CCC'GGG
Cuts at: 0 687 1221
Size: 687 534
SphI G_CATG'C
Cuts at: 0 955 1221
Size: 955 266
StuI AGG'CCT
Cuts at: 0 235 1221
Size: 235 986
StyI C'CwwG_G
Cuts at: 0 239 1118 1221
Size: 239 879 103
Fragments arranged by size:
879 239 103
TatI w'GTAC_w
Cuts at: 0 896 1043 1221
Size: 896 147 178
Fragments arranged by size:
896 178 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 732 873 1077 1221
Size: 372 360 141 204 144
Fragments arranged by size:
372 360 204 144 141
XmaI C'CCGG_G
Cuts at: 0 685 1221
Size: 685 536
Enzymes that do cut:
AccI AcuI AleI AlfI ApaI ApaLI AvaI BanII
BbsI BfrBI BglI BglII Bme1580I BplI BpmI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PasI PflMI PpuMI
PspOMI PvuII SacI SapI SgrAI SmaI SphI StuI
StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaHI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmlI SnaBI SpeI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI