Restriction map for
pF1KB6602
(Linear) (Six-base) MAPSORT of: KIBB6602.seq Check: 9068 from: 1 to: 1290
KIBB6602.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 967 1290
Size: 144 823 323
Fragments arranged by size:
823 323 144
AgeI A'CCGG_T
Cuts at: 0 38 1290
Size: 38 1252
AhdI GACnn_n'nnGTC
Cuts at: 0 364 904 1290
Size: 364 540 386
Fragments arranged by size:
540 386 364
AleI CACnn'nnGTG
Cuts at: 0 838 1290
Size: 838 452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 1219 1290
Size: 469 750 71
Fragments arranged by size:
750 469 71
AlwNI CAG_nnn'CTG
Cuts at: 0 115 374 1290
Size: 115 259 916
Fragments arranged by size:
916 259 115
ApaI G_GGCC'C
Cuts at: 0 762 1290
Size: 762 528
AvaI C'yCGr_G
Cuts at: 0 1055 1290
Size: 1055 235
BamHI G'GATC_C
Cuts at: 0 1019 1290
Size: 1019 271
BanI G'GyrC_C
Cuts at: 0 60 73 1290
Size: 60 13 1217
Fragments arranged by size:
1217 60 13
BanII G_rGCy'C
Cuts at: 0 52 124 414 554 762 1290
Size: 52 72 290 140 208 528
Fragments arranged by size:
528 290 208 140 72 52
BbeI G_GCGC'C
Cuts at: 0 64 77 1290
Size: 64 13 1213
Fragments arranged by size:
1213 64 13
BbvCI CC'TCA_GC
Cuts at: 0 860 1290
Size: 860 430
BglII A'GATC_T
Cuts at: 0 416 1290
Size: 416 874
Bme1580I G_kGCm'C
Cuts at: 0 762 1290
Size: 762 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 343 396 616 1138 1290
Size: 322 21 53 220 522 152
Fragments arranged by size:
522 322 220 152 53 21
Bpu10I CC'TnA_GC
Cuts at: 0 860 1290
Size: 860 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1054 1290
Size: 601 453 236
Fragments arranged by size:
601 453 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 298 818 1255 1290
Size: 238 60 520 437 35
Fragments arranged by size:
520 437 238 60 35
BsaHI Gr'CG_yC
Cuts at: 0 61 74 1290
Size: 61 13 1216
Fragments arranged by size:
1216 61 13
BsaWI w'CCGG_w
Cuts at: 0 38 314 1290
Size: 38 276 976
Fragments arranged by size:
976 276 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 916 946 1290
Size: 916 30 344
Fragments arranged by size:
916 344 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 637 808 841 1290
Size: 309 328 171 33 449
Fragments arranged by size:
449 328 309 171 33
BseYI C'CCAG_C
Cuts at: 0 64 115 1290
Size: 64 51 1175
Fragments arranged by size:
1175 64 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1290
Size: 1012 278
BsiEI CG_ry'CG
Cuts at: 0 82 267 1290
Size: 82 185 1023
Fragments arranged by size:
1023 185 82
BsiHKAI G_wGCw'C
Cuts at: 0 344 835 1290
Size: 344 491 455
Fragments arranged by size:
491 455 344
BsmBI CGTCTCn'nnnn_
Cuts at: 0 871 891 1290
Size: 871 20 399
Fragments arranged by size:
871 399 20
Bsp1286I G_dGCh'C
Cuts at: 0 52 124 344 414 554 762 835 1290
Size: 52 72 220 70 140 208 73 455
Fragments arranged by size:
455 220 208 140 73 72 70 52
BspHI T'CATG_A
Cuts at: 0 635 1290
Size: 635 655
BsrBI CCG'CTC
Cuts at: 0 149 1290
Size: 149 1141
BsrFI r'CCGG_y
Cuts at: 0 38 81 1290
Size: 38 43 1209
Fragments arranged by size:
1209 43 38
BstEII G'GTnAC_C
Cuts at: 0 843 1290
Size: 843 447
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1290
Size: 744 546
BstYI r'GATC_y
Cuts at: 0 134 416 1019 1290
Size: 134 282 603 271
Fragments arranged by size:
603 282 271 134
Bsu36I CC'TnA_GG
Cuts at: 0 406 970 1290
Size: 406 564 320
Fragments arranged by size:
564 406 320
BtgI C'CryG_G
Cuts at: 0 546 701 1290
Size: 546 155 589
Fragments arranged by size:
589 546 155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 413 448 1290
Size: 413 35 842
Fragments arranged by size:
842 413 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 493 1290
Size: 493 797
EaeI y'GGCC_r
Cuts at: 0 79 95 188 515 1290
Size: 79 16 93 327 775
Fragments arranged by size:
775 327 93 79 16
EagI C'GGCC_G
Cuts at: 0 79 1290
Size: 79 1211
EarI CTCTTCn'nnn_
Cuts at: 0 637 647 1290
Size: 637 10 643
Fragments arranged by size:
643 637 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 322 343 396 616 967 1138 1290
Size: 144 178 21 53 220 351 171 152
Fragments arranged by size:
351 220 178 171 152 144 53 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1290
Size: 973 317
EcoO109I rG'GnC_Cy
Cuts at: 0 183 358 402 729 1092 1290
Size: 183 175 44 327 363 198
Fragments arranged by size:
363 327 198 183 175 44
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 972 1290
Size: 972 318
HaeII r_GCGC'y
Cuts at: 0 64 77 116 1074 1290
Size: 64 13 39 958 216
Fragments arranged by size:
958 216 64 39 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 350 382 597 629 890 916 922 948
Size: 350 32 215 32 261 26 6 26
Cuts at: 948 1290
Size: 342
Fragments arranged by size:
350 342 261 215 32 32 26 26
6
HincII GTy'rAC
Cuts at: 0 698 1290
Size: 698 592
KasI G'GCGC_C
Cuts at: 0 60 73 1290
Size: 60 13 1217
Fragments arranged by size:
1217 60 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1290
Size: 846 444
MscI TGG'CCA
Cuts at: 0 190 517 1290
Size: 190 327 773
Fragments arranged by size:
773 327 190
MslI CAynn'nnrTG
Cuts at: 0 838 1290
Size: 838 452
MspA1I CmG'CkG
Cuts at: 0 594 1290
Size: 594 696
NarI GG'CG_CC
Cuts at: 0 61 74 1290
Size: 61 13 1216
Fragments arranged by size:
1216 61 13
NcoI C'CATG_G
Cuts at: 0 546 1290
Size: 546 744
PasI CC'CwG_GG
Cuts at: 0 45 1290
Size: 45 1245
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 915 1290
Size: 531 384 375
Fragments arranged by size:
531 384 375
PfoI T'CCnGG_A
Cuts at: 0 130 335 1290
Size: 130 205 955
Fragments arranged by size:
955 205 130
PpuMI rG'GwC_Cy
Cuts at: 0 358 402 729 1290
Size: 358 44 327 561
Fragments arranged by size:
561 358 327 44
PspOMI G'GGCC_C
Cuts at: 0 758 1290
Size: 758 532
PstI C_TGCA'G
Cuts at: 0 458 593 1290
Size: 458 135 697
Fragments arranged by size:
697 458 135
PvuII CAG'CTG
Cuts at: 0 594 1290
Size: 594 696
SanDI GG'GwC_CC
Cuts at: 0 358 1290
Size: 358 932
SapI GCTCTTCn'nnn_
Cuts at: 0 637 1290
Size: 637 653
ScaI AGT'ACT
Cuts at: 0 808 1290
Size: 808 482
SfcI C'TryA_G
Cuts at: 0 454 589 997 1290
Size: 454 135 408 293
Fragments arranged by size:
454 408 293 135
SfoI GGC'GCC
Cuts at: 0 62 75 1290
Size: 62 13 1215
Fragments arranged by size:
1215 62 13
SmaI CCC'GGG
Cuts at: 0 1057 1290
Size: 1057 233
SmlI C'TyrA_G
Cuts at: 0 616 1033 1290
Size: 616 417 257
Fragments arranged by size:
616 417 257
StuI AGG'CCT
Cuts at: 0 668 1290
Size: 668 622
StyI C'CwwG_G
Cuts at: 0 546 914 1184 1290
Size: 546 368 270 106
Fragments arranged by size:
546 368 270 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 1290
Size: 322 968
TatI w'GTAC_w
Cuts at: 0 806 1290
Size: 806 484
XmaI C'CCGG_G
Cuts at: 0 1055 1290
Size: 1055 235
Enzymes that do cut:
AcuI AgeI AhdI AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbvCI BglII Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI
BstEII BstXI BstYI Bsu36I BtgI CspCI DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SanDI
SapI ScaI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI BsaAI BsaBI BsiWI BsmI BspEI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI BtsI ClaI DraI DraIII EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI