(Linear) (Six-base) MAPSORT of: KIBB6603.seq  Check: 2058  from: 1  to: 1224



KIBB6603.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0     138    1224 
   Size:        138    1086
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     964    1224 
   Size:        314     650     260

  Fragments arranged by size: 

                 650     314     260
 
ApoI r'AATT_y

Cuts at:      0     160     496     703    1087    1224 
   Size:        160     336     207     384     137

  Fragments arranged by size: 

                 384     336     207     160     137
 
BanI G'GyrC_C

Cuts at:      0     138    1224 
   Size:        138    1086
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     965    1224 
   Size:        965     259
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     547     581    1224 
   Size:        547      34     643

  Fragments arranged by size: 

                 643     547      34
 
BclI T'GATC_A

Cuts at:      0     723    1224 
   Size:        723     501
 
BglI GCCn_nnn'nGGC

Cuts at:      0      40    1224 
   Size:         40    1184
 
BglII A'GATC_T

Cuts at:      0     331    1224 
   Size:        331     893
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1224 
   Size:         58    1166
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     278     499    1211    1224 
   Size:        278     221     712      13

  Fragments arranged by size: 

                 712     278     221      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     812    1224 
   Size:        812     412
 
BseYI C'CCAG_C

Cuts at:      0     134    1037    1224 
   Size:        134     903     187

  Fragments arranged by size: 

                 903     187     134
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     545    1188    1224 
   Size:        545     643      36

  Fragments arranged by size: 

                 643     545      36
 
BsrDI GCAATG_nn'

Cuts at:      0     433    1224 
   Size:        433     791
 
BstEII G'GTnAC_C

Cuts at:      0     874    1224 
   Size:        874     350
 
BstYI r'GATC_y

Cuts at:      0     331    1224 
   Size:        331     893
 
BtgI C'CryG_G

Cuts at:      0      91    1224 
   Size:         91    1133
 
DraI TTT'AAA

Cuts at:      0     825    1008    1224 
   Size:        825     183     216

  Fragments arranged by size: 

                 825     216     183
 
EaeI y'GGCC_r

Cuts at:      0     513    1224 
   Size:        513     711
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     314     964    1224 
   Size:         58     256     650     260

  Fragments arranged by size: 

                 650     260     256      58
 
KpnI G_GTAC'C

Cuts at:      0     142    1224 
   Size:        142    1082
 
MscI TGG'CCA

Cuts at:      0     515    1224 
   Size:        515     709
 
ScaI AGT'ACT

Cuts at:      0     410     914    1224 
   Size:        410     504     310

  Fragments arranged by size: 

                 504     410     310
 
SfcI C'TryA_G

Cuts at:      0     732    1224 
   Size:        732     492
 
SspI AAT'ATT

Cuts at:      0     417    1224 
   Size:        417     807
 
StyI C'CwwG_G

Cuts at:      0     224     836    1224 
   Size:        224     612     388

  Fragments arranged by size: 

                 612     388     224
 
TatI w'GTAC_w

Cuts at:      0     408     521     912    1062    1224 
   Size:        408     113     391     150     162

  Fragments arranged by size: 

                 408     391     162     150     113
 
Tth111I GACn'n_nGTC

Cuts at:      0     790    1224 
   Size:        790     434
 
XbaI T'CTAG_A

Cuts at:      0     675    1224 
   Size:        675     549
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     843    1045    1224 
   Size:        843     202     179

  Fragments arranged by size: 

                 843     202     179
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI     BanI     BbsI     BcgI     BclI     BglI
    BglII     BpmI     BsaI    BseRI    BseYI    BspMI    BsrDI   BstEII
    BstYI     BtgI     DraI     EaeI  Eco57MI     KpnI     MscI     ScaI
     SfcI     SspI     StyI     TatI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TstI     XhoI     XmaI     XmnI     ZraI