Restriction map for
pF1KB6605
(Linear) (Six-base) MAPSORT of: KIBB6605.seq Check: 3703 from: 1 to: 1179
KIBB6605.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 334 649 752 980 1179
Size: 112 222 315 103 228 199
Fragments arranged by size:
315 228 222 199 112 103
AflII C'TTAA_G
Cuts at: 0 301 1179
Size: 301 878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 1179
Size: 223 956
AlwNI CAG_nnn'CTG
Cuts at: 0 763 1179
Size: 763 416
ApaI G_GGCC'C
Cuts at: 0 145 1179
Size: 145 1034
ApoI r'AATT_y
Cuts at: 0 448 574 868 1179
Size: 448 126 294 311
Fragments arranged by size:
448 311 294 126
BanI G'GyrC_C
Cuts at: 0 1094 1179
Size: 1094 85
BanII G_rGCy'C
Cuts at: 0 145 1179
Size: 145 1034
BbeI G_GCGC'C
Cuts at: 0 1098 1179
Size: 1098 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 384 1179
Size: 339 45 795
Fragments arranged by size:
795 339 45
BclI T'GATC_A
Cuts at: 0 339 1179
Size: 339 840
BglII A'GATC_T
Cuts at: 0 601 1179
Size: 601 578
Bme1580I G_kGCm'C
Cuts at: 0 11 145 1179
Size: 11 134 1034
Fragments arranged by size:
1034 134 11
BmgBI CAC'GTC
Cuts at: 0 529 1054 1081 1179
Size: 529 525 27 98
Fragments arranged by size:
529 525 98 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 1179
Size: 678 501
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 179 1179
Size: 179 1000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1179
Size: 1039 140
Bpu10I CC'TnA_GC
Cuts at: 0 164 1179
Size: 164 1015
BsaI GGTCTCn'nnnn_
Cuts at: 0 1028 1179
Size: 1028 151
BsaHI Gr'CG_yC
Cuts at: 0 1095 1179
Size: 1095 84
BsaWI w'CCGG_w
Cuts at: 0 986 1179
Size: 986 193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 557 814 1179
Size: 292 265 257 365
Fragments arranged by size:
365 292 265 257
BseYI C'CCAG_C
Cuts at: 0 250 1167 1179
Size: 250 917 12
Fragments arranged by size:
917 250 12
BsmI GAATG_Cn'
Cuts at: 0 1162 1179
Size: 1162 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 274 1016 1179
Size: 274 742 163
Fragments arranged by size:
742 274 163
Bsp1286I G_dGCh'C
Cuts at: 0 11 145 1179
Size: 11 134 1034
Fragments arranged by size:
1034 134 11
BspEI T'CCGG_A
Cuts at: 0 986 1179
Size: 986 193
BsrFI r'CCGG_y
Cuts at: 0 121 1179
Size: 121 1058
BssSI C'ACGA_G
Cuts at: 0 398 560 634 1179
Size: 398 162 74 545
Fragments arranged by size:
545 398 162 74
BstBI TT'CG_AA
Cuts at: 0 413 1179
Size: 413 766
BstEII G'GTnAC_C
Cuts at: 0 36 1179
Size: 36 1143
BstYI r'GATC_y
Cuts at: 0 601 658 1179
Size: 601 57 521
Fragments arranged by size:
601 521 57
BtgI C'CryG_G
Cuts at: 0 170 293 522 1104 1179
Size: 170 123 229 582 75
Fragments arranged by size:
582 229 170 123 75
BtsI GCAGTG_nn'
Cuts at: 0 913 1179
Size: 913 266
DraIII CAC_nnn'GTG
Cuts at: 0 400 1179
Size: 400 779
DrdI GACnn_nn'nnGTC
Cuts at: 0 1122 1179
Size: 1122 57
EaeI y'GGCC_r
Cuts at: 0 1041 1179
Size: 1041 138
EarI CTCTTCn'nnn_
Cuts at: 0 501 782 941 1179
Size: 501 281 159 238
Fragments arranged by size:
501 281 238 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 334 649 752 980 1039 1179
Size: 112 222 315 103 228 59 140
Fragments arranged by size:
315 228 222 140 112 103 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 485 1179
Size: 485 694
EcoO109I rG'GnC_Cy
Cuts at: 0 141 142 1179
Size: 141 1 1037
Fragments arranged by size:
1037 141 1
FspI TGC'GCA
Cuts at: 0 211 1179
Size: 211 968
HaeII r_GCGC'y
Cuts at: 0 301 1098 1179
Size: 301 797 81
Fragments arranged by size:
797 301 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 994 1026 1179
Size: 55 32 907 32 153
Fragments arranged by size:
907 153 55 32 32
HincII GTy'rAC
Cuts at: 0 267 1179
Size: 267 912
KasI G'GCGC_C
Cuts at: 0 1094 1179
Size: 1094 85
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 306 1179
Size: 212 94 873
Fragments arranged by size:
873 212 94
MslI CAynn'nnrTG
Cuts at: 0 620 1179
Size: 620 559
MspA1I CmG'CkG
Cuts at: 0 172 207 243 1017 1179
Size: 172 35 36 774 162
Fragments arranged by size:
774 172 162 36 35
NaeI GCC'GGC
Cuts at: 0 123 1179
Size: 123 1056
NarI GG'CG_CC
Cuts at: 0 1095 1179
Size: 1095 84
NgoMIV G'CCGG_C
Cuts at: 0 121 1179
Size: 121 1058
PasI CC'CwG_GG
Cuts at: 0 145 1179
Size: 145 1034
PflMI CCAn_nnn'nTGG
Cuts at: 0 1054 1105 1179
Size: 1054 51 74
Fragments arranged by size:
1054 74 51
PspOMI G'GGCC_C
Cuts at: 0 141 1179
Size: 141 1038
PvuII CAG'CTG
Cuts at: 0 207 1017 1179
Size: 207 810 162
Fragments arranged by size:
810 207 162
SacII CC_GC'GG
Cuts at: 0 173 1179
Size: 173 1006
SfoI GGC'GCC
Cuts at: 0 1096 1179
Size: 1096 83
SmlI C'TyrA_G
Cuts at: 0 301 1179
Size: 301 878
StyI C'CwwG_G
Cuts at: 0 1038 1179
Size: 1038 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 480 491 1132 1179
Size: 480 11 641 47
Fragments arranged by size:
641 480 47 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1179
Size: 258 921
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI ApoI BanI BanII
BbeI BbsI BclI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsmI
BsmBI Bsp1286I BspEI BsrFI BssSI BstBI BstEII BstYI
BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII KasI MmeI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PspOMI PvuII
SacII SfoI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI
BlpI BmtI BpuEI BsaAI BsaBI BsaXI BsgI BsiEI
BsiHKAI BsiWI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BstAPI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI