Restriction map for
pF1KB6607
(Linear) (Six-base) MAPSORT of: KIBB6607.seq Check: 224 from: 1 to: 1227
KIBB6607.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 375 423 1227
Size: 375 48 804
Fragments arranged by size:
804 375 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 740 925 1157 1227
Size: 34 706 185 232 70
Fragments arranged by size:
706 232 185 70 34
AleI CACnn'nnGTG
Cuts at: 0 316 1227
Size: 316 911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 638 1227
Size: 638 589
AlwNI CAG_nnn'CTG
Cuts at: 0 277 490 766 1054 1227
Size: 277 213 276 288 173
Fragments arranged by size:
288 277 276 213 173
ApaLI G'TGCA_C
Cuts at: 0 545 1227
Size: 545 682
ApoI r'AATT_y
Cuts at: 0 829 1182 1227
Size: 829 353 45
Fragments arranged by size:
829 353 45
AseI AT'TA_AT
Cuts at: 0 136 1227
Size: 136 1091
AvaI C'yCGr_G
Cuts at: 0 55 1227
Size: 55 1172
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1141 1174 1227
Size: 1141 33 53
Fragments arranged by size:
1141 53 33
BanII G_rGCy'C
Cuts at: 0 747 1227
Size: 747 480
BbsI GAAGACnn'nnnn_
Cuts at: 0 22 746 1227
Size: 22 724 481
Fragments arranged by size:
724 481 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 901 935 1227
Size: 901 34 292
Fragments arranged by size:
901 292 34
Bme1580I G_kGCm'C
Cuts at: 0 549 1227
Size: 549 678
BmrI ACTGGGnnnn_n'
Cuts at: 0 749 1227
Size: 749 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 365 710 833 1227
Size: 7 358 345 123 394
Fragments arranged by size:
394 358 345 123 7
Bpu10I CC'TnA_GC
Cuts at: 0 323 1227
Size: 323 904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1227
Size: 710 517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 729 759 1227
Size: 729 30 468
Fragments arranged by size:
729 468 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 737 1031 1034 1227
Size: 73 664 294 3 193
Fragments arranged by size:
664 294 193 73 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 443 1227
Size: 395 48 784
Fragments arranged by size:
784 395 48
BsiHKAI G_wGCw'C
Cuts at: 0 549 1227
Size: 549 678
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 1227
Size: 55 1172
Bsp1286I G_dGCh'C
Cuts at: 0 549 747 1227
Size: 549 198 480
Fragments arranged by size:
549 480 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 375 417 423 1227
Size: 115 260 42 6 804
Fragments arranged by size:
804 260 115 42 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 544 766 1227
Size: 544 222 461
Fragments arranged by size:
544 461 222
BstEII G'GTnAC_C
Cuts at: 0 6 219 1227
Size: 6 213 1008
Fragments arranged by size:
1008 213 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 1227
Size: 318 909
Bsu36I CC'TnA_GG
Cuts at: 0 1091 1227
Size: 1091 136
BtsI GCAGTG_nn'
Cuts at: 0 242 668 767 1041 1227
Size: 242 426 99 274 186
Fragments arranged by size:
426 274 242 186 99
DrdI GACnn_nn'nnGTC
Cuts at: 0 561 1227
Size: 561 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 34 365 710 740 833 925 1157
Size: 7 27 331 345 30 93 92 232
Cuts at: 1157 1227
Size: 70
Fragments arranged by size:
345 331 232 93 92 70 30 27
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 484 516 577 609 1227
Size: 484 32 61 32 618
Fragments arranged by size:
618 484 61 32 32
HincII GTy'rAC
Cuts at: 0 717 1227
Size: 717 510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1227
Size: 355 872
MslI CAynn'nnrTG
Cuts at: 0 316 595 1227
Size: 316 279 632
Fragments arranged by size:
632 316 279
MspA1I CmG'CkG
Cuts at: 0 772 1227
Size: 772 455
NdeI CA'TA_TG
Cuts at: 0 1067 1227
Size: 1067 160
PstI C_TGCA'G
Cuts at: 0 447 771 777 1227
Size: 447 324 6 450
Fragments arranged by size:
450 447 324 6
PvuII CAG'CTG
Cuts at: 0 772 1227
Size: 772 455
ScaI AGT'ACT
Cuts at: 0 738 1227
Size: 738 489
SfcI C'TryA_G
Cuts at: 0 287 443 767 773 907 1106 1204 1227
Size: 287 156 324 6 134 199 98 23
Fragments arranged by size:
324 287 199 156 134 98 23 6
SmlI C'TyrA_G
Cuts at: 0 725 1227
Size: 725 502
StyI C'CwwG_G
Cuts at: 0 347 1227
Size: 347 880
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 506 1227
Size: 506 721
TatI w'GTAC_w
Cuts at: 0 496 736 1227
Size: 496 240 491
Fragments arranged by size:
496 491 240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1227
Size: 315 912
XmnI GAAnn'nnTTC
Cuts at: 0 992 1227
Size: 992 235
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaLI ApoI AseI
AvaI BaeI BanII BbsI BcgI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BstAPI BstEII BstXI Bsu36I BtsI DrdI Eco57MI
Hin4I HincII MmeI MslI MspA1I NdeI PstI PvuII
ScaI SfcI SmlI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AsiSI AvrII BamHI BanI
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI