Restriction map for
pF1KB6610
(Linear) (Six-base) MAPSORT of: KIBB6610.seq Check: 5399 from: 1 to: 1290
KIBB6610.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 389 1290
Size: 343 46 901
Fragments arranged by size:
901 343 46
AfeI AGC'GCT
Cuts at: 0 32 667 1290
Size: 32 635 623
Fragments arranged by size:
635 623 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 773 1076 1290
Size: 773 303 214
Fragments arranged by size:
773 303 214
AlwNI CAG_nnn'CTG
Cuts at: 0 536 907 1290
Size: 536 371 383
Fragments arranged by size:
536 383 371
ApaI G_GGCC'C
Cuts at: 0 333 1290
Size: 333 957
ApaLI G'TGCA_C
Cuts at: 0 763 1290
Size: 763 527
ApoI r'AATT_y
Cuts at: 0 232 1290
Size: 232 1058
AvaI C'yCGr_G
Cuts at: 0 768 1180 1290
Size: 768 412 110
Fragments arranged by size:
768 412 110
BanI G'GyrC_C
Cuts at: 0 409 951 1060 1290
Size: 409 542 109 230
Fragments arranged by size:
542 409 230 109
BanII G_rGCy'C
Cuts at: 0 87 170 333 478 850 1106 1121 1283
Size: 87 83 163 145 372 256 15 162
Cuts at: 1283 1290
Size: 7
Fragments arranged by size:
372 256 163 162 145 87 83 15
7
BbeI G_GCGC'C
Cuts at: 0 955 1290
Size: 955 335
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 1290
Size: 496 794
BbvCI CC'TCA_GC
Cuts at: 0 827 841 941 1290
Size: 827 14 100 349
Fragments arranged by size:
827 349 100 14
BglI GCCn_nnn'nGGC
Cuts at: 0 598 1253 1290
Size: 598 655 37
Fragments arranged by size:
655 598 37
BglII A'GATC_T
Cuts at: 0 930 1166 1290
Size: 930 236 124
Fragments arranged by size:
930 236 124
Bme1580I G_kGCm'C
Cuts at: 0 333 767 1128 1134 1290
Size: 333 434 361 6 156
Fragments arranged by size:
434 361 333 156 6
BmgBI CAC'GTC
Cuts at: 0 300 453 707 1290
Size: 300 153 254 583
Fragments arranged by size:
583 300 254 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 463 1290
Size: 90 373 827
Fragments arranged by size:
827 373 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 595 634 804 995 1290
Size: 169 426 39 170 191 295
Fragments arranged by size:
426 295 191 170 169 39
Bpu10I CC'TnA_GC
Cuts at: 0 13 827 841 941 1290
Size: 13 814 14 100 349
Fragments arranged by size:
814 349 100 14 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1290
Size: 900 390
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 1015 1290
Size: 874 141 275
Fragments arranged by size:
874 275 141
BsaAI yAC'GTr
Cuts at: 0 48 1275 1290
Size: 48 1227 15
Fragments arranged by size:
1227 48 15
BsaHI Gr'CG_yC
Cuts at: 0 952 1290
Size: 952 338
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 982 1012 1290
Size: 361 30 591 30 278
Fragments arranged by size:
591 361 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 178 373 571 574 610 858 953
Size: 166 12 195 198 3 36 248 95
Cuts at: 953 958 1003 1290
Size: 5 45 287
Fragments arranged by size:
287 248 198 195 166 95 45 36
12 5 3
BseYI C'CCAG_C
Cuts at: 0 955 1290
Size: 955 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 997 1069 1290
Size: 635 362 72 221
Fragments arranged by size:
635 362 221 72
BsiEI CG_ry'CG
Cuts at: 0 77 1290
Size: 77 1213
BsiHKAI G_wGCw'C
Cuts at: 0 170 767 1076 1283 1290
Size: 170 597 309 207 7
Fragments arranged by size:
597 309 207 170 7
Bsp1286I G_dGCh'C
Cuts at: 0 87 170 333 478 767 850 1076 1106
Size: 87 83 163 145 289 83 226 30
Cuts at: 1106 1121 1128 1134 1283 1290
Size: 15 7 6 149 7
Fragments arranged by size:
289 226 163 149 145 87 83 83
30 15 7 7 6
BsrDI GCAATG_nn'
Cuts at: 0 117 1290
Size: 117 1173
BsrFI r'CCGG_y
Cuts at: 0 948 1063 1290
Size: 948 115 227
Fragments arranged by size:
948 227 115
BstEII G'GTnAC_C
Cuts at: 0 57 1290
Size: 57 1233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1275 1290
Size: 1275 15
BstYI r'GATC_y
Cuts at: 0 930 1166 1290
Size: 930 236 124
Fragments arranged by size:
930 236 124
BtgI C'CryG_G
Cuts at: 0 308 1290
Size: 308 982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 417 452 1290
Size: 417 35 838
Fragments arranged by size:
838 417 35
EaeI y'GGCC_r
Cuts at: 0 311 395 438 1245 1290
Size: 311 84 43 807 45
Fragments arranged by size:
807 311 84 45 43
EarI CTCTTCn'nnn_
Cuts at: 0 980 1290
Size: 980 310
EcoICRI GAG'CTC
Cuts at: 0 168 1281 1290
Size: 168 1113 9
Fragments arranged by size:
1113 168 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 343 389 595 634 804 995 1290
Size: 169 174 46 206 39 170 191 295
Fragments arranged by size:
295 206 191 174 170 169 46 39
EcoO109I rG'GnC_Cy
Cuts at: 0 210 329 457 465 1290
Size: 210 119 128 8 825
Fragments arranged by size:
825 210 128 119 8
HaeII r_GCGC'y
Cuts at: 0 34 627 669 955 1290
Size: 34 593 42 286 335
Fragments arranged by size:
593 335 286 42 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 739 771 1290
Size: 202 32 505 32 519
Fragments arranged by size:
519 505 202 32 32
KasI G'GCGC_C
Cuts at: 0 951 1290
Size: 951 339
MscI TGG'CCA
Cuts at: 0 313 397 1247 1290
Size: 313 84 850 43
Fragments arranged by size:
850 313 84 43
MslI CAynn'nnrTG
Cuts at: 0 121 1204 1290
Size: 121 1083 86
Fragments arranged by size:
1083 121 86
MspA1I CmG'CkG
Cuts at: 0 176 606 612 904 1290
Size: 176 430 6 292 386
Fragments arranged by size:
430 386 292 176 6
NaeI GCC'GGC
Cuts at: 0 950 1065 1290
Size: 950 115 225
Fragments arranged by size:
950 225 115
NarI GG'CG_CC
Cuts at: 0 952 1290
Size: 952 338
NcoI C'CATG_G
Cuts at: 0 308 1290
Size: 308 982
NgoMIV G'CCGG_C
Cuts at: 0 948 1063 1290
Size: 948 115 227
Fragments arranged by size:
948 227 115
NspI r_CATG'y
Cuts at: 0 1260 1290
Size: 1260 30
PasI CC'CwG_GG
Cuts at: 0 923 1290
Size: 923 367
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 1290
Size: 453 837
PfoI T'CCnGG_A
Cuts at: 0 446 1290
Size: 446 844
PpuMI rG'GwC_Cy
Cuts at: 0 210 457 1290
Size: 210 247 833
Fragments arranged by size:
833 247 210
PspOMI G'GGCC_C
Cuts at: 0 329 1290
Size: 329 961
PstI C_TGCA'G
Cuts at: 0 202 860 1290
Size: 202 658 430
Fragments arranged by size:
658 430 202
PvuII CAG'CTG
Cuts at: 0 612 904 1290
Size: 612 292 386
Fragments arranged by size:
612 386 292
SacI G_AGCT'C
Cuts at: 0 170 1283 1290
Size: 170 1113 7
Fragments arranged by size:
1113 170 7
SanDI GG'GwC_CC
Cuts at: 0 457 1290
Size: 457 833
SfcI C'TryA_G
Cuts at: 0 198 417 856 1290
Size: 198 219 439 434
Fragments arranged by size:
439 434 219 198
SfoI GGC'GCC
Cuts at: 0 953 1290
Size: 953 337
SmlI C'TyrA_G
Cuts at: 0 915 1290
Size: 915 375
StuI AGG'CCT
Cuts at: 0 43 1290
Size: 43 1247
StyI C'CwwG_G
Cuts at: 0 308 398 1290
Size: 308 90 892
Fragments arranged by size:
892 308 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 886 1290
Size: 91 795 404
Fragments arranged by size:
795 404 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 361 393 1290
Size: 361 32 897
Fragments arranged by size:
897 361 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1105 1290
Size: 1105 185
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI
BsrFI BstEII BstXI BstYI BtgI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI
SfoI SmlI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BlpI BmtI BplI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI