Restriction map for
pF1KB6611
(Linear) (Six-base) MAPSORT of: KIBB6611.seq Check: 9868 from: 1 to: 1236
KIBB6611.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 981 1236
Size: 981 255
AflIII A'CryG_T
Cuts at: 0 917 1236
Size: 917 319
AgeI A'CCGG_T
Cuts at: 0 305 1236
Size: 305 931
AleI CACnn'nnGTG
Cuts at: 0 974 1236
Size: 974 262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 623 1236
Size: 623 613
AlwNI CAG_nnn'CTG
Cuts at: 0 63 1236
Size: 63 1173
ApoI r'AATT_y
Cuts at: 0 9 1236
Size: 9 1227
AvaI C'yCGr_G
Cuts at: 0 585 1236
Size: 585 651
BanI G'GyrC_C
Cuts at: 0 299 323 564 880 1236
Size: 299 24 241 316 356
Fragments arranged by size:
356 316 299 241 24
BanII G_rGCy'C
Cuts at: 0 404 1082 1163 1236
Size: 404 678 81 73
Fragments arranged by size:
678 404 81 73
BbeI G_GCGC'C
Cuts at: 0 884 1236
Size: 884 352
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 996 1236
Size: 32 964 240
Fragments arranged by size:
964 240 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 262 730 1236
Size: 262 468 506
Fragments arranged by size:
506 468 262
BglI GCCn_nnn'nGGC
Cuts at: 0 683 1236
Size: 683 553
BglII A'GATC_T
Cuts at: 0 863 1236
Size: 863 373
Bme1580I G_kGCm'C
Cuts at: 0 567 1236
Size: 567 669
BmgBI CAC'GTC
Cuts at: 0 918 1236
Size: 918 318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 684 1236
Size: 684 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 802 1236
Size: 593 209 434
Fragments arranged by size:
593 434 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 241 1236
Size: 223 18 995
Fragments arranged by size:
995 223 18
BsaAI yAC'GTr
Cuts at: 0 819 909 1236
Size: 819 90 327
Fragments arranged by size:
819 327 90
BsaHI Gr'CG_yC
Cuts at: 0 592 770 881 1236
Size: 592 178 111 355
Fragments arranged by size:
592 355 178 111
BsaWI w'CCGG_w
Cuts at: 0 305 1236
Size: 305 931
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 904 934 1236
Size: 904 30 302
Fragments arranged by size:
904 302 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 856 1090 1236
Size: 856 234 146
Fragments arranged by size:
856 234 146
BseYI C'CCAG_C
Cuts at: 0 389 764 1156 1236
Size: 389 375 392 80
Fragments arranged by size:
392 389 375 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 583 1236
Size: 376 207 653
Fragments arranged by size:
653 376 207
BsiEI CG_ry'CG
Cuts at: 0 873 1236
Size: 873 363
BsiHKAI G_wGCw'C
Cuts at: 0 217 404 1082 1129 1236
Size: 217 187 678 47 107
Fragments arranged by size:
678 217 187 107 47
BsmI GAATG_Cn'
Cuts at: 0 1201 1236
Size: 1201 35
Bsp1286I G_dGCh'C
Cuts at: 0 217 404 567 1082 1129 1163 1236
Size: 217 187 163 515 47 34 73
Fragments arranged by size:
515 217 187 163 73 47 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 981 995 1236
Size: 981 14 241
Fragments arranged by size:
981 241 14
BsrBI CCG'CTC
Cuts at: 0 852 1026 1236
Size: 852 174 210
Fragments arranged by size:
852 210 174
BsrFI r'CCGG_y
Cuts at: 0 305 1236
Size: 305 931
BsrGI T'GTAC_A
Cuts at: 0 482 920 995 1236
Size: 482 438 75 241
Fragments arranged by size:
482 438 241 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 1236
Size: 351 885
BstYI r'GATC_y
Cuts at: 0 158 863 1236
Size: 158 705 373
Fragments arranged by size:
705 373 158
BtgI C'CryG_G
Cuts at: 0 226 469 926 1112 1236
Size: 226 243 457 186 124
Fragments arranged by size:
457 243 226 186 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1023 1236
Size: 1023 213
BtsI GCAGTG_nn'
Cuts at: 0 595 1236
Size: 595 641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 686 721 1236
Size: 686 35 515
Fragments arranged by size:
686 515 35
DraI TTT'AAA
Cuts at: 0 14 1236
Size: 14 1222
DrdI GACnn_nn'nnGTC
Cuts at: 0 130 1236
Size: 130 1106
EaeI y'GGCC_r
Cuts at: 0 74 341 1236
Size: 74 267 895
Fragments arranged by size:
895 267 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1236
Size: 19 1217
EcoICRI GAG'CTC
Cuts at: 0 402 1080 1236
Size: 402 678 156
Fragments arranged by size:
678 402 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 802 1236
Size: 593 209 434
Fragments arranged by size:
593 434 209
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1236
Size: 523 713
EcoO109I rG'GnC_Cy
Cuts at: 0 60 1236
Size: 60 1176
EcoRV GAT'ATC
Cuts at: 0 199 1236
Size: 199 1037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 819 1236
Size: 819 417
HaeII r_GCGC'y
Cuts at: 0 382 884 1236
Size: 382 502 352
Fragments arranged by size:
502 382 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 716 748 1236
Size: 716 32 488
Fragments arranged by size:
716 488 32
HincII GTy'rAC
Cuts at: 0 966 1236
Size: 966 270
HindIII A'AGCT_T
Cuts at: 0 336 1236
Size: 336 900
KasI G'GCGC_C
Cuts at: 0 880 1236
Size: 880 356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1236
Size: 1166 70
MscI TGG'CCA
Cuts at: 0 76 343 1236
Size: 76 267 893
Fragments arranged by size:
893 267 76
MslI CAynn'nnrTG
Cuts at: 0 974 1102 1132 1236
Size: 974 128 30 104
Fragments arranged by size:
974 128 104 30
MspA1I CmG'CkG
Cuts at: 0 54 471 674 680 1236
Size: 54 417 203 6 556
Fragments arranged by size:
556 417 203 54 6
NarI GG'CG_CC
Cuts at: 0 881 1236
Size: 881 355
NspI r_CATG'y
Cuts at: 0 1101 1236
Size: 1101 135
PpuMI rG'GwC_Cy
Cuts at: 0 60 1236
Size: 60 1176
PstI C_TGCA'G
Cuts at: 0 25 59 992 1236
Size: 25 34 933 244
Fragments arranged by size:
933 244 34 25
PvuII CAG'CTG
Cuts at: 0 54 1236
Size: 54 1182
SacI G_AGCT'C
Cuts at: 0 404 1082 1236
Size: 404 678 154
Fragments arranged by size:
678 404 154
SacII CC_GC'GG
Cuts at: 0 472 1236
Size: 472 764
SbfI CC_TGCA'GG
Cuts at: 0 992 1236
Size: 992 244
SexAI A'CCwGG_T
Cuts at: 0 179 350 524 1236
Size: 179 171 174 712
Fragments arranged by size:
712 179 174 171
SfcI C'TryA_G
Cuts at: 0 21 55 437 988 1236
Size: 21 34 382 551 248
Fragments arranged by size:
551 382 248 34 21
SfoI GGC'GCC
Cuts at: 0 882 1236
Size: 882 354
SmaI CCC'GGG
Cuts at: 0 587 1236
Size: 587 649
SmlI C'TyrA_G
Cuts at: 0 238 256 1236
Size: 238 18 980
Fragments arranged by size:
980 238 18
SspI AAT'ATT
Cuts at: 0 175 1236
Size: 175 1061
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 582 1236
Size: 582 654
TatI w'GTAC_w
Cuts at: 0 482 920 995 1236
Size: 482 438 75 241
Fragments arranged by size:
482 438 241 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 1236
Size: 544 32 660
Fragments arranged by size:
660 544 32
Tth111I GACn'n_nGTC
Cuts at: 0 1212 1236
Size: 1212 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1149 1236
Size: 1149 87
XmaI C'CCGG_G
Cuts at: 0 585 1236
Size: 585 651
XmnI GAAnn'nnTTC
Cuts at: 0 802 1236
Size: 802 434
Enzymes that do cut:
AarI AflIII AgeI AleI AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BciVI BglI BglII Bme1580I
BmgBI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NarI NspI PpuMI PstI
PvuII SacI SacII SbfI SexAI SfcI SfoI SmaI
SmlI SspI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BlpI BmrI BmtI
Bpu10I BsaI BsaBI BsiWI BsmBI BspEI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
DraIII EagI EarI EcoRI FseI FspI FspAI HpaI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SanDI SapI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI StyI SwaI
XbaI XhoI ZraI