Restriction map for
pF1KB6612
(Linear) (Six-base) MAPSORT of: KIBB6612.seq Check: 9264 from: 1 to: 1296
KIBB6612.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 674 796 1296
Size: 164 510 122 500
Fragments arranged by size:
510 500 164 122
AlwNI CAG_nnn'CTG
Cuts at: 0 468 1153 1296
Size: 468 685 143
Fragments arranged by size:
685 468 143
ApaI G_GGCC'C
Cuts at: 0 8 485 647 1296
Size: 8 477 162 649
Fragments arranged by size:
649 477 162 8
ApoI r'AATT_y
Cuts at: 0 741 1296
Size: 741 555
AvaI C'yCGr_G
Cuts at: 0 412 902 1296
Size: 412 490 394
Fragments arranged by size:
490 412 394
BanII G_rGCy'C
Cuts at: 0 8 485 638 647 1296
Size: 8 477 153 9 649
Fragments arranged by size:
649 477 153 9 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1296
Size: 917 379
BciVI GTATCCnnnnn_n'
Cuts at: 0 420 742 1296
Size: 420 322 554
Fragments arranged by size:
554 420 322
BglI GCCn_nnn'nGGC
Cuts at: 0 23 602 1296
Size: 23 579 694
Fragments arranged by size:
694 579 23
BglII A'GATC_T
Cuts at: 0 935 1296
Size: 935 361
Bme1580I G_kGCm'C
Cuts at: 0 8 258 485 647 1296
Size: 8 250 227 162 649
Fragments arranged by size:
649 250 227 162 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1296
Size: 607 689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1296
Size: 481 815
BsaI GGTCTCn'nnnn_
Cuts at: 0 1231 1296
Size: 1231 65
BsaBI GATnn'nnATC
Cuts at: 0 40 1296
Size: 40 1256
BsaWI w'CCGG_w
Cuts at: 0 1008 1296
Size: 1008 288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 493 523 1296
Size: 213 30 250 30 773
Fragments arranged by size:
773 250 213 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 498 775 1047 1156 1296
Size: 498 277 272 109 140
Fragments arranged by size:
498 277 272 140 109
BseYI C'CCAG_C
Cuts at: 0 21 468 1135 1296
Size: 21 447 667 161
Fragments arranged by size:
667 447 161 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1296
Size: 563 733
BsiHKAI G_wGCw'C
Cuts at: 0 112 497 638 1296
Size: 112 385 141 658
Fragments arranged by size:
658 385 141 112
Bsp1286I G_dGCh'C
Cuts at: 0 8 112 258 485 497 638 647 1296
Size: 8 104 146 227 12 141 9 649
Fragments arranged by size:
649 227 146 141 104 12 9 8
BspEI T'CCGG_A
Cuts at: 0 1008 1296
Size: 1008 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 307 1296
Size: 307 989
BstAPI GCAn_nnn'nTGC
Cuts at: 0 226 1296
Size: 226 1070
BstBI TT'CG_AA
Cuts at: 0 1094 1296
Size: 1094 202
BstEII G'GTnAC_C
Cuts at: 0 1049 1296
Size: 1049 247
BstYI r'GATC_y
Cuts at: 0 339 935 1296
Size: 339 596 361
Fragments arranged by size:
596 361 339
BtgI C'CryG_G
Cuts at: 0 8 1221 1296
Size: 8 1213 75
Fragments arranged by size:
1213 75 8
BtsI GCAGTG_nn'
Cuts at: 0 246 428 1296
Size: 246 182 868
Fragments arranged by size:
868 246 182
DraIII CAC_nnn'GTG
Cuts at: 0 50 1296
Size: 50 1246
EaeI y'GGCC_r
Cuts at: 0 1215 1296
Size: 1215 81
EarI CTCTTCn'nnn_
Cuts at: 0 752 833 926 1296
Size: 752 81 93 370
Fragments arranged by size:
752 370 93 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 850 1296
Size: 850 446
EcoICRI GAG'CTC
Cuts at: 0 636 1296
Size: 636 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 607 674 796 1296
Size: 164 443 67 122 500
Fragments arranged by size:
500 443 164 122 67
EcoO109I rG'GnC_Cy
Cuts at: 0 4 481 482 643 644 765 1038 1296
Size: 4 477 1 161 1 121 273 258
Fragments arranged by size:
477 273 258 161 121 4 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 1158 1190 1296
Size: 493 32 633 32 106
Fragments arranged by size:
633 493 106 32 32
HindIII A'AGCT_T
Cuts at: 0 787 1296
Size: 787 509
MscI TGG'CCA
Cuts at: 0 1217 1296
Size: 1217 79
MspA1I CmG'CkG
Cuts at: 0 10 29 577 1296
Size: 10 19 548 719
Fragments arranged by size:
719 548 19 10
NspI r_CATG'y
Cuts at: 0 306 1296
Size: 306 990
PasI CC'CwG_GG
Cuts at: 0 640 1203 1296
Size: 640 563 93
Fragments arranged by size:
640 563 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 1296
Size: 468 828
PpuMI rG'GwC_Cy
Cuts at: 0 1038 1296
Size: 1038 258
PspOMI G'GGCC_C
Cuts at: 0 4 481 643 1296
Size: 4 477 162 653
Fragments arranged by size:
653 477 162 4
PstI C_TGCA'G
Cuts at: 0 576 1296
Size: 576 720
PvuII CAG'CTG
Cuts at: 0 577 1296
Size: 577 719
SacI G_AGCT'C
Cuts at: 0 638 1296
Size: 638 658
SacII CC_GC'GG
Cuts at: 0 11 1296
Size: 11 1285
SanDI GG'GwC_CC
Cuts at: 0 1038 1296
Size: 1038 258
ScaI AGT'ACT
Cuts at: 0 291 1296
Size: 291 1005
SfcI C'TryA_G
Cuts at: 0 572 1296
Size: 572 724
SmlI C'TyrA_G
Cuts at: 0 496 1296
Size: 496 800
SphI G_CATG'C
Cuts at: 0 306 1296
Size: 306 990
StyI C'CwwG_G
Cuts at: 0 735 1296
Size: 735 561
TatI w'GTAC_w
Cuts at: 0 289 947 1296
Size: 289 658 349
Fragments arranged by size:
658 349 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 761 793 962 994 1296
Size: 761 32 169 32 302
Fragments arranged by size:
761 302 169 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 809 1296
Size: 809 487
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BbsI BciVI
BglI BglII Bme1580I BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BstAPI BstBI BstEII BstYI BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I Hin4I HindIII MscI
MspA1I NspI PasI PflMI PpuMI PspOMI PstI PvuII
SacI SacII SanDI ScaI SfcI SmlI SphI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI