Restriction map for
pF1KB6613
(Linear) (Six-base) MAPSORT of: KIBB6613.seq Check: 5727 from: 1 to: 1236
KIBB6613.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 0 266 1236
Size: 266 970
AleI CACnn'nnGTG
Cuts at: 0 31 1236
Size: 31 1205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 448 1236
Size: 49 399 788
Fragments arranged by size:
788 399 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 949 981 1236
Size: 949 32 255
Fragments arranged by size:
949 255 32
ApaI G_GGCC'C
Cuts at: 0 1133 1236
Size: 1133 103
ApaLI G'TGCA_C
Cuts at: 0 121 1213 1236
Size: 121 1092 23
Fragments arranged by size:
1092 121 23
ApoI r'AATT_y
Cuts at: 0 106 1236
Size: 106 1130
AvaI C'yCGr_G
Cuts at: 0 21 260 469 606 1186 1236
Size: 21 239 209 137 580 50
Fragments arranged by size:
580 239 209 137 50 21
BanI G'GyrC_C
Cuts at: 0 25 895 1028 1236
Size: 25 870 133 208
Fragments arranged by size:
870 208 133 25
BanII G_rGCy'C
Cuts at: 0 61 261 1133 1236
Size: 61 200 872 103
Fragments arranged by size:
872 200 103 61
BciVI GTATCCnnnnn_n'
Cuts at: 0 405 1236
Size: 405 831
BclI T'GATC_A
Cuts at: 0 654 1236
Size: 654 582
BglII A'GATC_T
Cuts at: 0 401 1236
Size: 401 835
Bme1580I G_kGCm'C
Cuts at: 0 28 125 1033 1133 1217 1236
Size: 28 97 908 100 84 19
Fragments arranged by size:
908 100 97 84 28 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 637 1236
Size: 637 599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 423 1236
Size: 423 813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 820 1236
Size: 457 363 416
Fragments arranged by size:
457 416 363
BsaI GGTCTCn'nnnn_
Cuts at: 0 1142 1236
Size: 1142 94
BsaAI yAC'GTr
Cuts at: 0 201 636 1236
Size: 201 435 600
Fragments arranged by size:
600 435 201
BsaHI Gr'CG_yC
Cuts at: 0 995 1236
Size: 995 241
BsaWI w'CCGG_w
Cuts at: 0 500 821 1236
Size: 500 321 415
Fragments arranged by size:
500 415 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 1236
Size: 172 30 1034
Fragments arranged by size:
1034 172 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1114 1236
Size: 1114 122
BseYI C'CCAG_C
Cuts at: 0 973 1032 1236
Size: 973 59 204
Fragments arranged by size:
973 204 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 1236
Size: 723 513
BsiEI CG_ry'CG
Cuts at: 0 17 822 1090 1236
Size: 17 805 268 146
Fragments arranged by size:
805 268 146 17
BsiHKAI G_wGCw'C
Cuts at: 0 125 299 427 1217 1236
Size: 125 174 128 790 19
Fragments arranged by size:
790 174 128 125 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 724 1236
Size: 724 512
Bsp1286I G_dGCh'C
Cuts at: 0 28 61 125 261 299 427 1033 1133
Size: 28 33 64 136 38 128 606 100
Cuts at: 1133 1217 1236
Size: 84 19
Fragments arranged by size:
606 136 128 100 84 64 38 33
28 19
BspEI T'CCGG_A
Cuts at: 0 500 1236
Size: 500 736
BspHI T'CATG_A
Cuts at: 0 330 1236
Size: 330 906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 1183 1236
Size: 92 1091 53
Fragments arranged by size:
1091 92 53
BsrDI GCAATG_nn'
Cuts at: 0 515 1236
Size: 515 721
BstAPI GCAn_nnn'nTGC
Cuts at: 0 526 1236
Size: 526 710
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 1077 1236
Size: 786 291 159
Fragments arranged by size:
786 291 159
BstYI r'GATC_y
Cuts at: 0 401 1236
Size: 401 835
BtgI C'CryG_G
Cuts at: 0 29 1236
Size: 29 1207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 253 1236
Size: 253 983
DraI TTT'AAA
Cuts at: 0 366 1236
Size: 366 870
DraIII CAC_nnn'GTG
Cuts at: 0 1028 1236
Size: 1028 208
DrdI GACnn_nn'nnGTC
Cuts at: 0 211 1236
Size: 211 1025
EaeI y'GGCC_r
Cuts at: 0 14 47 1236
Size: 14 33 1189
Fragments arranged by size:
1189 33 14
EagI C'GGCC_G
Cuts at: 0 14 1236
Size: 14 1222
EarI CTCTTCn'nnn_
Cuts at: 0 70 859 1236
Size: 70 789 377
Fragments arranged by size:
789 377 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 820 1236
Size: 457 363 416
Fragments arranged by size:
457 416 363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 870 1236
Size: 156 714 366
Fragments arranged by size:
714 366 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1236
Size: 703 32 501
Fragments arranged by size:
703 501 32
HindIII A'AGCT_T
Cuts at: 0 315 1236
Size: 315 921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1070 1236
Size: 791 279 166
Fragments arranged by size:
791 279 166
MslI CAynn'nnrTG
Cuts at: 0 31 430 1054 1208 1236
Size: 31 399 624 154 28
Fragments arranged by size:
624 399 154 31 28
MspA1I CmG'CkG
Cuts at: 0 31 1236
Size: 31 1205
NotI GC'GGCC_GC
Cuts at: 0 14 1236
Size: 14 1222
PmlI CAC'GTG
Cuts at: 0 201 1236
Size: 201 1035
PspOMI G'GGCC_C
Cuts at: 0 1129 1236
Size: 1129 107
PspXI vC'TCGA_Gb
Cuts at: 0 260 1236
Size: 260 976
PstI C_TGCA'G
Cuts at: 0 89 1236
Size: 89 1147
RsrII CG'GwC_CG
Cuts at: 0 762 1236
Size: 762 474
SacII CC_GC'GG
Cuts at: 0 32 1236
Size: 32 1204
SapI GCTCTTCn'nnn_
Cuts at: 0 70 1236
Size: 70 1166
SexAI A'CCwGG_T
Cuts at: 0 113 1236
Size: 113 1123
SfcI C'TryA_G
Cuts at: 0 85 1236
Size: 85 1151
SmaI CCC'GGG
Cuts at: 0 23 1188 1236
Size: 23 1165 48
Fragments arranged by size:
1165 48 23
SmlI C'TyrA_G
Cuts at: 0 260 1236
Size: 260 976
StuI AGG'CCT
Cuts at: 0 187 1236
Size: 187 1049
StyI C'CwwG_G
Cuts at: 0 1070 1236
Size: 1070 166
TatI w'GTAC_w
Cuts at: 0 602 1013 1236
Size: 602 411 223
Fragments arranged by size:
602 411 223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 723 1140 1236
Size: 198 525 417 96
Fragments arranged by size:
525 417 198 96
XhoI C'TCGA_G
Cuts at: 0 260 1236
Size: 260 976
XmaI C'CCGG_G
Cuts at: 0 21 1186 1236
Size: 21 1165 50
Fragments arranged by size:
1165 50 21
XmnI GAAnn'nnTTC
Cuts at: 0 853 1236
Size: 853 383
Enzymes that do cut:
AccI AleI AlfI AloI ApaI ApaLI ApoI AvaI
BanI BanII BciVI BclI BglII Bme1580I BmrI BplI
BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BstAPI BstXI BstYI BtgI BtgZI DraI DraIII
DrdI EaeI EagI EarI Eco57MI FalI Hin4I HindIII
MmeI MslI MspA1I NotI PmlI PspOMI PspXI PstI
RsrII SacII SapI SexAI SfcI SmaI SmlI StuI
StyI TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BfrBI BglI BlpI
BmgBI BmtI Bpu10I BpuEI BsaBI BsiWI BsmI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtsI ClaI CspCI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI ZraI