Restriction map for
pF1KB6614
(Linear) (Six-base) MAPSORT of: KIBB6614.seq Check: 1701 from: 1 to: 1296
KIBB6614.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 356 1296
Size: 356 940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 810 1296
Size: 673 137 486
Fragments arranged by size:
673 486 137
AgeI A'CCGG_T
Cuts at: 0 1203 1296
Size: 1203 93
AhdI GACnn_n'nnGTC
Cuts at: 0 1214 1296
Size: 1214 82
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1296
Size: 1157 139
AlwNI CAG_nnn'CTG
Cuts at: 0 534 1032 1296
Size: 534 498 264
Fragments arranged by size:
534 498 264
ApaI G_GGCC'C
Cuts at: 0 380 1296
Size: 380 916
AvaI C'yCGr_G
Cuts at: 0 154 372 1296
Size: 154 218 924
Fragments arranged by size:
924 218 154
BanI G'GyrC_C
Cuts at: 0 106 356 1296
Size: 106 250 940
Fragments arranged by size:
940 250 106
BanII G_rGCy'C
Cuts at: 0 49 380 908 1296
Size: 49 331 528 388
Fragments arranged by size:
528 388 331 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 1126 1296
Size: 1126 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 135 169 1296
Size: 135 34 1127
Fragments arranged by size:
1127 135 34
BglI GCCn_nnn'nGGC
Cuts at: 0 62 490 1296
Size: 62 428 806
Fragments arranged by size:
806 428 62
BglII A'GATC_T
Cuts at: 0 896 1296
Size: 896 400
BlpI GC'TnA_GC
Cuts at: 0 952 1296
Size: 952 344
Bme1580I G_kGCm'C
Cuts at: 0 312 380 1296
Size: 312 68 916
Fragments arranged by size:
916 312 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 156 263 334 355 631 991 1150
Size: 15 141 107 71 21 276 360 159
Cuts at: 1150 1183 1296
Size: 33 113
Fragments arranged by size:
360 276 159 141 113 107 71 33
21 15
Bpu10I CC'TnA_GC
Cuts at: 0 745 1296
Size: 745 551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1296
Size: 616 680
BsaWI w'CCGG_w
Cuts at: 0 1203 1296
Size: 1203 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 39 331 430 652 682 685 1063
Size: 18 21 292 99 222 30 3 378
Cuts at: 1063 1254 1296
Size: 191 42
Fragments arranged by size:
378 292 222 191 99 42 30 21
18 3
BseYI C'CCAG_C
Cuts at: 0 123 947 1061 1296
Size: 123 824 114 235
Fragments arranged by size:
824 235 123 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1030 1296
Size: 898 132 266
Fragments arranged by size:
898 266 132
BsiHKAI G_wGCw'C
Cuts at: 0 908 1296
Size: 908 388
Bsp1286I G_dGCh'C
Cuts at: 0 49 312 380 908 1296
Size: 49 263 68 528 388
Fragments arranged by size:
528 388 263 68 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1266 1296
Size: 1266 30
BsrFI r'CCGG_y
Cuts at: 0 86 109 1203 1296
Size: 86 23 1094 93
Fragments arranged by size:
1094 93 86 23
BssSI C'ACGA_G
Cuts at: 0 664 1296
Size: 664 632
BstAPI GCAn_nnn'nTGC
Cuts at: 0 83 534 1296
Size: 83 451 762
Fragments arranged by size:
762 451 83
BstEII G'GTnAC_C
Cuts at: 0 1206 1296
Size: 1206 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 700 1296
Size: 700 596
BstYI r'GATC_y
Cuts at: 0 127 344 431 896 1022 1296
Size: 127 217 87 465 126 274
Fragments arranged by size:
465 274 217 127 126 87
BtgI C'CryG_G
Cuts at: 0 446 1296
Size: 446 850
EaeI y'GGCC_r
Cuts at: 0 596 857 1296
Size: 596 261 439
Fragments arranged by size:
596 439 261
EarI CTCTTCn'nnn_
Cuts at: 0 1232 1296
Size: 1232 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4 1000 1296
Size: 4 996 296
Fragments arranged by size:
996 296 4
EcoICRI GAG'CTC
Cuts at: 0 906 1296
Size: 906 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 156 263 334 355 631 673 810
Size: 15 141 107 71 21 276 42 137
Cuts at: 810 991 1150 1183 1296
Size: 181 159 33 113
Fragments arranged by size:
276 181 159 141 137 113 107 71
42 33 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 316 634 1162 1296
Size: 298 18 318 528 134
Fragments arranged by size:
528 318 298 134 18
EcoO109I rG'GnC_Cy
Cuts at: 0 369 376 531 1296
Size: 369 7 155 765
Fragments arranged by size:
765 369 155 7
FspI TGC'GCA
Cuts at: 0 571 1296
Size: 571 725
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 928 960 1200 1232 1296
Size: 928 32 240 32 64
Fragments arranged by size:
928 240 64 32 32
KpnI G_GTAC'C
Cuts at: 0 360 1296
Size: 360 936
MfeI C'AATT_G
Cuts at: 0 410 1296
Size: 410 886
MscI TGG'CCA
Cuts at: 0 598 859 1296
Size: 598 261 437
Fragments arranged by size:
598 437 261
MspA1I CmG'CkG
Cuts at: 0 176 1014 1056 1296
Size: 176 838 42 240
Fragments arranged by size:
838 240 176 42
NaeI GCC'GGC
Cuts at: 0 88 111 1296
Size: 88 23 1185
Fragments arranged by size:
1185 88 23
NgoMIV G'CCGG_C
Cuts at: 0 86 109 1296
Size: 86 23 1187
Fragments arranged by size:
1187 86 23
PfoI T'CCnGG_A
Cuts at: 0 1244 1296
Size: 1244 52
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 805 837 1296
Size: 805 32 459
Fragments arranged by size:
805 459 32
PspOMI G'GGCC_C
Cuts at: 0 376 1296
Size: 376 920
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1296
Size: 1081 32 183
Fragments arranged by size:
1081 183 32
PstI C_TGCA'G
Cuts at: 0 121 425 944 1296
Size: 121 304 519 352
Fragments arranged by size:
519 352 304 121
PvuII CAG'CTG
Cuts at: 0 176 1014 1056 1296
Size: 176 838 42 240
Fragments arranged by size:
838 240 176 42
SacI G_AGCT'C
Cuts at: 0 908 1296
Size: 908 388
SbfI CC_TGCA'GG
Cuts at: 0 121 1296
Size: 121 1175
ScaI AGT'ACT
Cuts at: 0 400 1296
Size: 400 896
SfcI C'TryA_G
Cuts at: 0 117 180 390 402 421 469 940 1296
Size: 117 63 210 12 19 48 471 356
Fragments arranged by size:
471 356 210 117 63 48 19 12
SmaI CCC'GGG
Cuts at: 0 374 1296
Size: 374 922
SmlI C'TyrA_G
Cuts at: 0 631 1296
Size: 631 665
StuI AGG'CCT
Cuts at: 0 925 1296
Size: 925 371
StyI C'CwwG_G
Cuts at: 0 926 1296
Size: 926 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 688 1296
Size: 94 594 608
Fragments arranged by size:
608 594 94
TatI w'GTAC_w
Cuts at: 0 398 1190 1296
Size: 398 792 106
Fragments arranged by size:
792 398 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 606 870 924 1033 1296
Size: 330 276 264 54 109 263
Fragments arranged by size:
330 276 264 263 109 54
XmaI C'CCGG_G
Cuts at: 0 372 1296
Size: 372 924
Enzymes that do cut:
Acc65I AcuI AgeI AhdI AlfI AlwNI ApaI AvaI
BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I
BpmI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI
BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI Hin4I KpnI MfeI MscI MspA1I NaeI NgoMIV
PfoI PpiI PspOMI PsrI PstI PvuII SacI SbfI
ScaI SfcI SmaI SmlI StuI StyI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI
FspAI HaeII HincII HindIII HpaI KasI MluI MmeI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SapI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI