Restriction map for
pF1KB6618
(Linear) (Six-base) MAPSORT of: KIBB6618.seq Check: 4568 from: 1 to: 1299
KIBB6618.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 483 1299
Size: 483 816
AleI CACnn'nnGTG
Cuts at: 0 1125 1299
Size: 1125 174
AlwNI CAG_nnn'CTG
Cuts at: 0 1174 1299
Size: 1174 125
ApoI r'AATT_y
Cuts at: 0 443 1024 1264 1299
Size: 443 581 240 35
Fragments arranged by size:
581 443 240 35
AseI AT'TA_AT
Cuts at: 0 176 1299
Size: 176 1123
AvaI C'yCGr_G
Cuts at: 0 932 1299
Size: 932 367
BamHI G'GATC_C
Cuts at: 0 981 1299
Size: 981 318
BanI G'GyrC_C
Cuts at: 0 16 387 1299
Size: 16 371 912
Fragments arranged by size:
912 371 16
BanII G_rGCy'C
Cuts at: 0 72 185 762 849 1299
Size: 72 113 577 87 450
Fragments arranged by size:
577 450 113 87 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 1299
Size: 37 1262
BclI T'GATC_A
Cuts at: 0 378 905 1299
Size: 378 527 394
Fragments arranged by size:
527 394 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 887 1299
Size: 244 643 412
Fragments arranged by size:
643 412 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1299
Size: 151 1148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1268 1299
Size: 1268 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1149 1299
Size: 904 245 150
Fragments arranged by size:
904 245 150
BsiHKAI G_wGCw'C
Cuts at: 0 72 118 185 1197 1299
Size: 72 46 67 1012 102
Fragments arranged by size:
1012 102 72 67 46
Bsp1286I G_dGCh'C
Cuts at: 0 72 118 185 762 849 1197 1299
Size: 72 46 67 577 87 348 102
Fragments arranged by size:
577 348 102 87 72 67 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 1299
Size: 483 816
BsrDI GCAATG_nn'
Cuts at: 0 454 1299
Size: 454 845
BsrGI T'GTAC_A
Cuts at: 0 292 735 1299
Size: 292 443 564
Fragments arranged by size:
564 443 292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 230 363 1299
Size: 230 133 936
Fragments arranged by size:
936 230 133
BstYI r'GATC_y
Cuts at: 0 724 981 1299
Size: 724 257 318
Fragments arranged by size:
724 318 257
DraIII CAC_nnn'GTG
Cuts at: 0 673 1299
Size: 673 626
EaeI y'GGCC_r
Cuts at: 0 788 956 1299
Size: 788 168 343
Fragments arranged by size:
788 343 168
EarI CTCTTCn'nnn_
Cuts at: 0 494 1299
Size: 494 805
EcoICRI GAG'CTC
Cuts at: 0 70 183 1299
Size: 70 113 1116
Fragments arranged by size:
1116 113 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 887 1299
Size: 244 643 412
Fragments arranged by size:
643 412 244
EcoO109I rG'GnC_Cy
Cuts at: 0 321 1299
Size: 321 978
EcoRV GAT'ATC
Cuts at: 0 1101 1299
Size: 1101 198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 826 1242 1299
Size: 826 416 57
Fragments arranged by size:
826 416 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1299
Size: 1059 32 208
Fragments arranged by size:
1059 208 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1030 1299
Size: 878 152 269
Fragments arranged by size:
878 269 152
MscI TGG'CCA
Cuts at: 0 790 958 1299
Size: 790 168 341
Fragments arranged by size:
790 341 168
MslI CAynn'nnrTG
Cuts at: 0 1125 1299
Size: 1125 174
MspA1I CmG'CkG
Cuts at: 0 27 1299
Size: 27 1272
PpuMI rG'GwC_Cy
Cuts at: 0 321 1299
Size: 321 978
PspXI vC'TCGA_Gb
Cuts at: 0 932 1299
Size: 932 367
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 260 292 1299
Size: 260 32 1007
Fragments arranged by size:
1007 260 32
PstI C_TGCA'G
Cuts at: 0 246 1299
Size: 246 1053
SacI G_AGCT'C
Cuts at: 0 72 185 1299
Size: 72 113 1114
Fragments arranged by size:
1114 113 72
SfcI C'TryA_G
Cuts at: 0 242 1299
Size: 242 1057
SmlI C'TyrA_G
Cuts at: 0 166 932 1299
Size: 166 766 367
Fragments arranged by size:
766 367 166
SspI AAT'ATT
Cuts at: 0 942 1299
Size: 942 357
StyI C'CwwG_G
Cuts at: 0 609 626 1299
Size: 609 17 673
Fragments arranged by size:
673 609 17
TatI w'GTAC_w
Cuts at: 0 292 735 1299
Size: 292 443 564
Fragments arranged by size:
564 443 292
Tth111I GACn'n_nGTC
Cuts at: 0 1127 1299
Size: 1127 172
XbaI T'CTAG_A
Cuts at: 0 1041 1299
Size: 1041 258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 227 363 492 1299
Size: 69 158 136 129 807
Fragments arranged by size:
807 158 136 129 69
XhoI C'TCGA_G
Cuts at: 0 932 1299
Size: 932 367
XmnI GAAnn'nnTTC
Cuts at: 0 1222 1299
Size: 1222 77
Enzymes that do cut:
AarI AleI AlwNI ApoI AseI AvaI BamHI BanI
BanII BbsI BclI BpmI BpuEI BseRI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrGI BstXI BstYI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I MmeI
MscI MslI MspA1I PpuMI PspXI PsrI PstI SacI
SfcI SmlI SspI StyI TatI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
XmaI ZraI