Restriction map for
pF1KB6622
(Linear) (Six-base) MAPSORT of: KIBB6622.seq Check: 1195 from: 1 to: 1308
KIBB6622.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1308
Size: 1157 151
AflII C'TTAA_G
Cuts at: 0 138 1308
Size: 138 1170
AflIII A'CryG_T
Cuts at: 0 577 1308
Size: 577 731
AleI CACnn'nnGTG
Cuts at: 0 140 1308
Size: 140 1168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 1308
Size: 841 467
ApoI r'AATT_y
Cuts at: 0 65 478 654 1179 1308
Size: 65 413 176 525 129
Fragments arranged by size:
525 413 176 129 65
AseI AT'TA_AT
Cuts at: 0 1150 1308
Size: 1150 158
AvaI C'yCGr_G
Cuts at: 0 203 1308
Size: 203 1105
BglII A'GATC_T
Cuts at: 0 900 1308
Size: 900 408
BmrI ACTGGGnnnn_n'
Cuts at: 0 451 1308
Size: 451 857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 1308
Size: 198 1110
BsaAI yAC'GTr
Cuts at: 0 73 1308
Size: 73 1235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 365 395 1308
Size: 107 30 228 30 913
Fragments arranged by size:
913 228 107 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1308
Size: 530 778
BsmI GAATG_Cn'
Cuts at: 0 1242 1308
Size: 1242 66
BspHI T'CATG_A
Cuts at: 0 1115 1308
Size: 1115 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 717 833 1308
Size: 717 116 475
Fragments arranged by size:
717 475 116
BsrDI GCAATG_nn'
Cuts at: 0 998 1308
Size: 998 310
BsrFI r'CCGG_y
Cuts at: 0 635 1308
Size: 635 673
BsrGI T'GTAC_A
Cuts at: 0 951 1308
Size: 951 357
BstEII G'GTnAC_C
Cuts at: 0 436 1308
Size: 436 872
BstYI r'GATC_y
Cuts at: 0 900 1308
Size: 900 408
BtgI C'CryG_G
Cuts at: 0 168 1160 1308
Size: 168 992 148
Fragments arranged by size:
992 168 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 860 1308
Size: 860 448
BtsI GCAGTG_nn'
Cuts at: 0 1285 1308
Size: 1285 23
DraI TTT'AAA
Cuts at: 0 428 690 860 1004 1308
Size: 428 262 170 144 304
Fragments arranged by size:
428 304 262 170 144
DraIII CAC_nnn'GTG
Cuts at: 0 44 1308
Size: 44 1264
EarI CTCTTCn'nnn_
Cuts at: 0 1016 1308
Size: 1016 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1308
Size: 1157 151
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 1308
Size: 52 1256
EcoRI G'AATT_C
Cuts at: 0 654 1308
Size: 654 654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 254 1308
Size: 254 1054
HindIII A'AGCT_T
Cuts at: 0 390 422 1308
Size: 390 32 886
Fragments arranged by size:
886 390 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1308
Size: 351 957
MslI CAynn'nnrTG
Cuts at: 0 140 296 1004 1308
Size: 140 156 708 304
Fragments arranged by size:
708 304 156 140
MspA1I CmG'CkG
Cuts at: 0 1200 1308
Size: 1200 108
NcoI C'CATG_G
Cuts at: 0 168 1160 1308
Size: 168 992 148
Fragments arranged by size:
992 168 148
NspI r_CATG'y
Cuts at: 0 581 843 1308
Size: 581 262 465
Fragments arranged by size:
581 465 262
PciI A'CATG_T
Cuts at: 0 577 1308
Size: 577 731
PsiI TTA'TAA
Cuts at: 0 985 1094 1308
Size: 985 109 214
Fragments arranged by size:
985 214 109
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 272 304 1308
Size: 272 32 1004
Fragments arranged by size:
1004 272 32
PstI C_TGCA'G
Cuts at: 0 311 714 1247 1308
Size: 311 403 533 61
Fragments arranged by size:
533 403 311 61
PvuII CAG'CTG
Cuts at: 0 1200 1308
Size: 1200 108
SapI GCTCTTCn'nnn_
Cuts at: 0 1016 1308
Size: 1016 292
ScaI AGT'ACT
Cuts at: 0 794 1308
Size: 794 514
SfcI C'TryA_G
Cuts at: 0 307 710 796 1243 1265 1308
Size: 307 403 86 447 22 43
Fragments arranged by size:
447 403 307 86 43 22
SmlI C'TyrA_G
Cuts at: 0 138 1308
Size: 138 1170
SspI AAT'ATT
Cuts at: 0 686 948 1308
Size: 686 262 360
Fragments arranged by size:
686 360 262
StyI C'CwwG_G
Cuts at: 0 168 1160 1308
Size: 168 992 148
Fragments arranged by size:
992 168 148
SwaI ATTT'AAAT
Cuts at: 0 1004 1308
Size: 1004 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 455 896 1308
Size: 455 441 412
Fragments arranged by size:
455 441 412
TatI w'GTAC_w
Cuts at: 0 123 792 951 1308
Size: 123 669 159 357
Fragments arranged by size:
669 357 159 123
XmnI GAAnn'nnTTC
Cuts at: 0 755 805 1308
Size: 755 50 503
Fragments arranged by size:
755 503 50
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI ApoI AseI AvaI
BglII BmrI BplI BsaAI BsaXI BsgI BsmI BspHI
BspMI BsrDI BsrFI BsrGI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII EarI Eco57MI EcoNI EcoRI FalI
HindIII MmeI MslI MspA1I NcoI NspI PciI PsiI
PsrI PstI PvuII SapI ScaI SfcI SmlI SspI
StyI SwaI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CspCI
DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRV FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI