Restriction map for
pF1KB6624
(Linear) (Six-base) MAPSORT of: KIBB6624.seq Check: 1884 from: 1 to: 1299
KIBB6624.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 412 1299
Size: 412 887
AccI GT'mk_AC
Cuts at: 0 368 1292 1299
Size: 368 924 7
Fragments arranged by size:
924 368 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1299
Size: 533 766
ApoI r'AATT_y
Cuts at: 0 178 1299
Size: 178 1121
AvrII C'CTAG_G
Cuts at: 0 990 1299
Size: 990 309
BamHI G'GATC_C
Cuts at: 0 252 1102 1299
Size: 252 850 197
Fragments arranged by size:
850 252 197
BanII G_rGCy'C
Cuts at: 0 809 953 1299
Size: 809 144 346
Fragments arranged by size:
809 346 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 682 1211 1299
Size: 682 529 88
Fragments arranged by size:
682 529 88
BbvCI CC'TCA_GC
Cuts at: 0 691 1299
Size: 691 608
BfrBI ATG'CAT
Cuts at: 0 293 1299
Size: 293 1006
BglII A'GATC_T
Cuts at: 0 512 1145 1299
Size: 512 633 154
Fragments arranged by size:
633 512 154
BlpI GC'TnA_GC
Cuts at: 0 952 1299
Size: 952 347
Bme1580I G_kGCm'C
Cuts at: 0 418 1299
Size: 418 881
BmrI ACTGGGnnnn_n'
Cuts at: 0 412 1299
Size: 412 887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1299
Size: 622 677
Bpu10I CC'TnA_GC
Cuts at: 0 248 691 1299
Size: 248 443 608
Fragments arranged by size:
608 443 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1299
Size: 284 1015
BsaWI w'CCGG_w
Cuts at: 0 332 1150 1299
Size: 332 818 149
Fragments arranged by size:
818 332 149
BseYI C'CCAG_C
Cuts at: 0 430 1299
Size: 430 869
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 66 1299
Size: 53 13 1233
Fragments arranged by size:
1233 53 13
BsiEI CG_ry'CG
Cuts at: 0 97 1299
Size: 97 1202
BsiHKAI G_wGCw'C
Cuts at: 0 809 953 1299
Size: 809 144 346
Fragments arranged by size:
809 346 144
BsmI GAATG_Cn'
Cuts at: 0 850 1299
Size: 850 449
BsmBI CGTCTCn'nnnn_
Cuts at: 0 179 1299
Size: 179 1120
Bsp1286I G_dGCh'C
Cuts at: 0 418 809 953 1299
Size: 418 391 144 346
Fragments arranged by size:
418 391 346 144
BspEI T'CCGG_A
Cuts at: 0 1150 1299
Size: 1150 149
BspHI T'CATG_A
Cuts at: 0 651 1176 1299
Size: 651 525 123
Fragments arranged by size:
651 525 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 412 794 1299
Size: 412 382 505
Fragments arranged by size:
505 412 382
BsrDI GCAATG_nn'
Cuts at: 0 293 534 685 1299
Size: 293 241 151 614
Fragments arranged by size:
614 293 241 151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 413 1129 1299
Size: 413 716 170
Fragments arranged by size:
716 413 170
BstYI r'GATC_y
Cuts at: 0 240 252 512 1102 1145 1299
Size: 240 12 260 590 43 154
Fragments arranged by size:
590 260 240 154 43 12
BstZ17I GTA'TAC
Cuts at: 0 1293 1299
Size: 1293 6
BtgI C'CryG_G
Cuts at: 0 68 1299
Size: 68 1231
BtsI GCAGTG_nn'
Cuts at: 0 385 418 600 1059 1299
Size: 385 33 182 459 240
Fragments arranged by size:
459 385 240 182 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 160 1299
Size: 160 1139
EcoICRI GAG'CTC
Cuts at: 0 807 951 1299
Size: 807 144 348
Fragments arranged by size:
807 348 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 622 1299
Size: 533 89 677
Fragments arranged by size:
677 533 89
EcoO109I rG'GnC_Cy
Cuts at: 0 388 645 1299
Size: 388 257 654
Fragments arranged by size:
654 388 257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 870 1299
Size: 870 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 986 1018 1299
Size: 986 32 281
Fragments arranged by size:
986 281 32
HincII GTy'rAC
Cuts at: 0 1077 1299
Size: 1077 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 144 1038 1299
Size: 77 67 894 261
Fragments arranged by size:
894 261 77 67
MslI CAynn'nnrTG
Cuts at: 0 656 1052 1299
Size: 656 396 247
Fragments arranged by size:
656 396 247
MspA1I CmG'CkG
Cuts at: 0 64 70 1299
Size: 64 6 1229
Fragments arranged by size:
1229 64 6
NsiI A_TGCA'T
Cuts at: 0 295 1299
Size: 295 1004
NspI r_CATG'y
Cuts at: 0 497 593 1299
Size: 497 96 706
Fragments arranged by size:
706 497 96
PfoI T'CCnGG_A
Cuts at: 0 213 1299
Size: 213 1086
PpuMI rG'GwC_Cy
Cuts at: 0 388 1299
Size: 388 911
PshAI GACnn'nnGTC
Cuts at: 0 316 1299
Size: 316 983
PstI C_TGCA'G
Cuts at: 0 84 318 1299
Size: 84 234 981
Fragments arranged by size:
981 234 84
PvuII CAG'CTG
Cuts at: 0 64 1299
Size: 64 1235
SacI G_AGCT'C
Cuts at: 0 809 953 1299
Size: 809 144 346
Fragments arranged by size:
809 346 144
SacII CC_GC'GG
Cuts at: 0 71 1299
Size: 71 1228
SanDI GG'GwC_CC
Cuts at: 0 388 1299
Size: 388 911
SfcI C'TryA_G
Cuts at: 0 80 314 1133 1299
Size: 80 234 819 166
Fragments arranged by size:
819 234 166 80
SmlI C'TyrA_G
Cuts at: 0 4 299 1299
Size: 4 295 1000
Fragments arranged by size:
1000 295 4
SphI G_CATG'C
Cuts at: 0 593 1299
Size: 593 706
SspI AAT'ATT
Cuts at: 0 960 1299
Size: 960 339
StuI AGG'CCT
Cuts at: 0 696 1299
Size: 696 603
StyI C'CwwG_G
Cuts at: 0 845 990 1299
Size: 845 145 309
Fragments arranged by size:
845 309 145
TatI w'GTAC_w
Cuts at: 0 565 1299
Size: 565 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 195 227 1299
Size: 195 32 1072
Fragments arranged by size:
1072 195 32
XmnI GAAnn'nnTTC
Cuts at: 0 178 1299
Size: 178 1121
Enzymes that do cut:
AarI AccI AcuI ApoI AvrII BamHI BanII BbsI
BbvCI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstYI BstZ17I
BtgI BtsI DrdI EcoICRI Eco57MI EcoO109I FalI Hin4I
HincII MmeI MslI MspA1I NsiI NspI PfoI PpuMI
PshAI PstI PvuII SacI SacII SanDI SfcI SmlI
SphI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BanI BbeI BcgI BciVI BclI
BglI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsiWI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI
DraIII EaeI EagI EarI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI