Restriction map for
pF1KB6625
(Linear) (Six-base) MAPSORT of: KIBB6625.seq Check: 1429 from: 1 to: 1218
KIBB6625.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 396 1218
Size: 396 822
AatII G_ACGT'C
Cuts at: 0 869 1218
Size: 869 349
AclI AA'CG_TT
Cuts at: 0 59 1218
Size: 59 1159
ApaI G_GGCC'C
Cuts at: 0 534 901 1218
Size: 534 367 317
Fragments arranged by size:
534 367 317
ApaLI G'TGCA_C
Cuts at: 0 436 1218
Size: 436 782
AvaI C'yCGr_G
Cuts at: 0 135 624 1029 1218
Size: 135 489 405 189
Fragments arranged by size:
489 405 189 135
BamHI G'GATC_C
Cuts at: 0 697 1218
Size: 697 521
BanI G'GyrC_C
Cuts at: 0 1156 1218
Size: 1156 62
BanII G_rGCy'C
Cuts at: 0 534 901 1010 1081 1105 1218
Size: 534 367 109 71 24 113
Fragments arranged by size:
534 367 113 109 71 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1218
Size: 500 718
BbvCI CC'TCA_GC
Cuts at: 0 166 410 1218
Size: 166 244 808
Fragments arranged by size:
808 244 166
BlpI GC'TnA_GC
Cuts at: 0 1004 1218
Size: 1004 214
Bme1580I G_kGCm'C
Cuts at: 0 440 534 901 1159 1187 1218
Size: 440 94 367 258 28 31
Fragments arranged by size:
440 367 258 94 31 28
BmgBI CAC'GTC
Cuts at: 0 1049 1218
Size: 1049 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 280 397 651 668 789 1218
Size: 148 132 117 254 17 121 429
Fragments arranged by size:
429 254 148 132 121 117 17
Bpu10I CC'TnA_GC
Cuts at: 0 166 410 1218
Size: 166 244 808
Fragments arranged by size:
808 244 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 739 1218
Size: 235 504 479
Fragments arranged by size:
504 479 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 377 819 982 1218
Size: 377 442 163 236
Fragments arranged by size:
442 377 236 163
BsaAI yAC'GTr
Cuts at: 0 549 1218
Size: 549 669
BsaBI GATnn'nnATC
Cuts at: 0 278 1218
Size: 278 940
BsaHI Gr'CG_yC
Cuts at: 0 718 826 866 1218
Size: 718 108 40 352
Fragments arranged by size:
718 352 108 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 1218
Size: 502 30 686
Fragments arranged by size:
686 502 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 465 1086 1089 1218
Size: 465 621 3 129
Fragments arranged by size:
621 465 129 3
BseYI C'CCAG_C
Cuts at: 0 726 1088 1199 1218
Size: 726 362 111 19
Fragments arranged by size:
726 362 111 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1218
Size: 64 1154
BsiEI CG_ry'CG
Cuts at: 0 1181 1218
Size: 1181 37
BsiHKAI G_wGCw'C
Cuts at: 0 136 440 1081 1218
Size: 136 304 641 137
Fragments arranged by size:
641 304 137 136
BsmI GAATG_Cn'
Cuts at: 0 243 1218
Size: 243 975
Bsp1286I G_dGCh'C
Cuts at: 0 136 440 534 901 1010 1081 1105 1159
Size: 136 304 94 367 109 71 24 54
Cuts at: 1159 1187 1218
Size: 28 31
Fragments arranged by size:
367 304 136 109 94 71 54 31
28 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 396 1218
Size: 396 822
BsrFI r'CCGG_y
Cuts at: 0 656 1176 1218
Size: 656 520 42
Fragments arranged by size:
656 520 42
BstYI r'GATC_y
Cuts at: 0 119 697 1218
Size: 119 578 521
Fragments arranged by size:
578 521 119
BtgI C'CryG_G
Cuts at: 0 147 320 431 1218
Size: 147 173 111 787
Fragments arranged by size:
787 173 147 111
BtsI GCAGTG_nn'
Cuts at: 0 1175 1218
Size: 1175 43
DrdI GACnn_nn'nnGTC
Cuts at: 0 426 1023 1218
Size: 426 597 195
Fragments arranged by size:
597 426 195
EaeI y'GGCC_r
Cuts at: 0 2 353 450 1178 1218
Size: 2 351 97 728 40
Fragments arranged by size:
728 351 97 40 2
EagI C'GGCC_G
Cuts at: 0 1178 1218
Size: 1178 40
EcoICRI GAG'CTC
Cuts at: 0 1079 1218
Size: 1079 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 280 397 651 668 789 1218
Size: 148 132 117 254 17 121 429
Fragments arranged by size:
429 254 148 132 121 117 17
EcoO109I rG'GnC_Cy
Cuts at: 0 530 897 898 1218
Size: 530 367 1 320
Fragments arranged by size:
530 367 320 1
FspI TGC'GCA
Cuts at: 0 764 1218
Size: 764 454
HaeII r_GCGC'y
Cuts at: 0 686 1218
Size: 686 532
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 502 534 787 819 898 930
Size: 393 32 77 32 253 32 79 32
Cuts at: 930 1218
Size: 288
Fragments arranged by size:
393 288 253 79 77 32 32 32
32
MscI TGG'CCA
Cuts at: 0 4 452 1218
Size: 4 448 766
Fragments arranged by size:
766 448 4
MspA1I CmG'CkG
Cuts at: 0 149 322 941 1043 1088 1218
Size: 149 173 619 102 45 130
Fragments arranged by size:
619 173 149 130 102 45
NaeI GCC'GGC
Cuts at: 0 1178 1218
Size: 1178 40
NcoI C'CATG_G
Cuts at: 0 431 1218
Size: 431 787
NgoMIV G'CCGG_C
Cuts at: 0 1176 1218
Size: 1176 42
PasI CC'CwG_GG
Cuts at: 0 534 1218
Size: 534 684
PfoI T'CCnGG_A
Cuts at: 0 257 1218
Size: 257 961
PspOMI G'GGCC_C
Cuts at: 0 530 897 1218
Size: 530 367 321
Fragments arranged by size:
530 367 321
PvuII CAG'CTG
Cuts at: 0 1043 1088 1218
Size: 1043 45 130
Fragments arranged by size:
1043 130 45
SacI G_AGCT'C
Cuts at: 0 1081 1218
Size: 1081 137
SacII CC_GC'GG
Cuts at: 0 150 323 1218
Size: 150 173 895
Fragments arranged by size:
895 173 150
SmlI C'TyrA_G
Cuts at: 0 214 754 1218
Size: 214 540 464
Fragments arranged by size:
540 464 214
StyI C'CwwG_G
Cuts at: 0 431 674 830 902 1218
Size: 431 243 156 72 316
Fragments arranged by size:
431 316 243 156 72
TatI w'GTAC_w
Cuts at: 0 305 620 1218
Size: 305 315 598
Fragments arranged by size:
598 315 305
ZraI GAC'GTC
Cuts at: 0 867 1218
Size: 867 351
Enzymes that do cut:
AarI AatII AclI ApaI ApaLI AvaI BamHI BanI
BanII BbsI BbvCI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstYI
BtgI BtsI DrdI EaeI EagI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I MscI MspA1I NaeI NcoI NgoMIV
PasI PfoI PspOMI PvuII SacI SacII SmlI StyI
TatI ZraI
Enzymes that do not cut:
AccI Acc65I AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmrI BmtI BplI BsaWI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI