Restriction map for
pF1KB6627
(Linear) (Six-base) MAPSORT of: KIBB6627.seq Check: 8519 from: 1 to: 1245
KIBB6627.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 0 125 467 605 920 1245
Size: 125 342 138 315 325
Fragments arranged by size:
342 325 315 138 125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 578 923 1076 1121 1245
Size: 157 421 345 153 45 124
Fragments arranged by size:
421 345 157 153 124 45
AlwNI CAG_nnn'CTG
Cuts at: 0 1111 1245
Size: 1111 134
AseI AT'TA_AT
Cuts at: 0 506 1245
Size: 506 739
BanII G_rGCy'C
Cuts at: 0 136 1245
Size: 136 1109
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 746 1245
Size: 158 588 499
Fragments arranged by size:
588 499 158
BciVI GTATCCnnnnn_n'
Cuts at: 0 570 724 1245
Size: 570 154 521
Fragments arranged by size:
570 521 154
BfrBI ATG'CAT
Cuts at: 0 346 1197 1245
Size: 346 851 48
Fragments arranged by size:
851 346 48
BlpI GC'TnA_GC
Cuts at: 0 680 1245
Size: 680 565
Bme1580I G_kGCm'C
Cuts at: 0 1099 1245
Size: 1099 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 924 1245
Size: 924 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 200 797 1245
Size: 120 80 597 448
Fragments arranged by size:
597 448 120 80
BsaHI Gr'CG_yC
Cuts at: 0 992 1245
Size: 992 253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 1245
Size: 118 30 1097
Fragments arranged by size:
1097 118 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 1105 1245
Size: 438 667 140
Fragments arranged by size:
667 438 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1245
Size: 503 742
BsmI GAATG_Cn'
Cuts at: 0 603 1245
Size: 603 642
Bsp1286I G_dGCh'C
Cuts at: 0 136 1099 1245
Size: 136 963 146
Fragments arranged by size:
963 146 136
BsrBI CCG'CTC
Cuts at: 0 323 1245
Size: 323 922
BsrGI T'GTAC_A
Cuts at: 0 693 1245
Size: 693 552
BstAPI GCAn_nnn'nTGC
Cuts at: 0 295 343 1245
Size: 295 48 902
Fragments arranged by size:
902 295 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 834 1245
Size: 120 714 411
Fragments arranged by size:
714 411 120
BstYI r'GATC_y
Cuts at: 0 6 251 961 1245
Size: 6 245 710 284
Fragments arranged by size:
710 284 245 6
BstZ17I GTA'TAC
Cuts at: 0 921 1245
Size: 921 324
BtgI C'CryG_G
Cuts at: 0 94 113 119 1073 1245
Size: 94 19 6 954 172
Fragments arranged by size:
954 172 94 19 6
EaeI y'GGCC_r
Cuts at: 0 116 161 1245
Size: 116 45 1084
Fragments arranged by size:
1084 116 45
EarI CTCTTCn'nnn_
Cuts at: 0 88 938 1245
Size: 88 850 307
Fragments arranged by size:
850 307 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 157 200 578 797 923 1076 1121
Size: 120 37 43 378 219 126 153 45
Cuts at: 1121 1245
Size: 124
Fragments arranged by size:
378 219 153 126 124 120 45 43
37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1245
Size: 1010 235
MscI TGG'CCA
Cuts at: 0 118 163 1245
Size: 118 45 1082
Fragments arranged by size:
1082 118 45
MslI CAynn'nnrTG
Cuts at: 0 118 982 1245
Size: 118 864 263
Fragments arranged by size:
864 263 118
MspA1I CmG'CkG
Cuts at: 0 233 1111 1245
Size: 233 878 134
Fragments arranged by size:
878 233 134
NcoI C'CATG_G
Cuts at: 0 94 113 119 1073 1245
Size: 94 19 6 954 172
Fragments arranged by size:
954 172 94 19 6
NdeI CA'TA_TG
Cuts at: 0 771 1245
Size: 771 474
NsiI A_TGCA'T
Cuts at: 0 348 1199 1245
Size: 348 851 46
Fragments arranged by size:
851 348 46
NspI r_CATG'y
Cuts at: 0 792 1245
Size: 792 453
PflMI CCAn_nnn'nTGG
Cuts at: 0 775 1074 1245
Size: 775 299 171
Fragments arranged by size:
775 299 171
PfoI T'CCnGG_A
Cuts at: 0 410 1245
Size: 410 835
PstI C_TGCA'G
Cuts at: 0 300 1245
Size: 300 945
PvuII CAG'CTG
Cuts at: 0 233 1245
Size: 233 1012
SapI GCTCTTCn'nnn_
Cuts at: 0 938 1245
Size: 938 307
SfcI C'TryA_G
Cuts at: 0 296 566 1245
Size: 296 270 679
Fragments arranged by size:
679 296 270
SspI AAT'ATT
Cuts at: 0 442 1245
Size: 442 803
StyI C'CwwG_G
Cuts at: 0 94 113 119 164 1073 1245
Size: 94 19 6 45 909 172
Fragments arranged by size:
909 172 94 45 19 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 1245
Size: 88 1157
TatI w'GTAC_w
Cuts at: 0 693 704 1245
Size: 693 11 541
Fragments arranged by size:
693 541 11
Tth111I GACn'n_nGTC
Cuts at: 0 431 1245
Size: 431 814
XbaI T'CTAG_A
Cuts at: 0 759 1245
Size: 759 486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 117 126 588 1245
Size: 117 9 462 657
Fragments arranged by size:
657 462 117 9
XmnI GAAnn'nnTTC
Cuts at: 0 82 422 1245
Size: 82 340 823
Fragments arranged by size:
823 340 82
Enzymes that do cut:
AccI AcuI AlwNI AseI BanII BbsI BciVI BfrBI
BlpI Bme1580I BmrI BpmI BsaHI BsaXI BseRI BsgI
BsmI Bsp1286I BsrBI BsrGI BstAPI BstXI BstYI BstZ17I
BtgI EaeI EarI Eco57MI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PflMI PfoI PstI PvuII
SapI SfcI SspI StyI TaqII TatI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BglI BglII BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII
BssSI BstBI BstEII Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TstI XhoI
XmaI ZraI