Restriction map for
pF1KB6635
(Linear) (Six-base) MAPSORT of: KIBB6635.seq Check: 8338 from: 1 to: 1254
KIBB6635.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 123 1254
Size: 123 1131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1254
Size: 1210 44
AflII C'TTAA_G
Cuts at: 0 933 1254
Size: 933 321
AleI CACnn'nnGTG
Cuts at: 0 752 1254
Size: 752 502
AlwNI CAG_nnn'CTG
Cuts at: 0 420 1218 1254
Size: 420 798 36
Fragments arranged by size:
798 420 36
ApoI r'AATT_y
Cuts at: 0 175 207 250 397 519 1084 1161 1254
Size: 175 32 43 147 122 565 77 93
Fragments arranged by size:
565 175 147 122 93 77 43 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 457 522 596 905 1254
Size: 293 164 65 74 309 349
Fragments arranged by size:
349 309 293 164 74 65
BfrBI ATG'CAT
Cuts at: 0 465 715 1254
Size: 465 250 539
Fragments arranged by size:
539 465 250
BglII A'GATC_T
Cuts at: 0 108 1254
Size: 108 1146
Bpu10I CC'TnA_GC
Cuts at: 0 953 1254
Size: 953 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1254
Size: 929 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 617 647 1254
Size: 617 30 607
Fragments arranged by size:
617 607 30
BsiHKAI G_wGCw'C
Cuts at: 0 671 1254
Size: 671 583
BsmI GAATG_Cn'
Cuts at: 0 1150 1254
Size: 1150 104
Bsp1286I G_dGCh'C
Cuts at: 0 671 1254
Size: 671 583
BspHI T'CATG_A
Cuts at: 0 264 1254
Size: 264 990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 286 1254
Size: 123 163 968
Fragments arranged by size:
968 163 123
BsrBI CCG'CTC
Cuts at: 0 162 1254
Size: 162 1092
BsrDI GCAATG_nn'
Cuts at: 0 199 1254
Size: 199 1055
BsrFI r'CCGG_y
Cuts at: 0 13 1254
Size: 13 1241
BssHII G'CGCG_C
Cuts at: 0 3 1254
Size: 3 1251
BssSI C'ACGA_G
Cuts at: 0 881 1254
Size: 881 373
BstYI r'GATC_y
Cuts at: 0 108 742 957 1254
Size: 108 634 215 297
Fragments arranged by size:
634 297 215 108
BtgI C'CryG_G
Cuts at: 0 84 1254
Size: 84 1170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 475 1254
Size: 475 779
BtsI GCAGTG_nn'
Cuts at: 0 1241 1254
Size: 1241 13
DraI TTT'AAA
Cuts at: 0 213 518 600 923 1254
Size: 213 305 82 323 331
Fragments arranged by size:
331 323 305 213 82
EarI CTCTTCn'nnn_
Cuts at: 0 71 504 1134 1254
Size: 71 433 630 120
Fragments arranged by size:
630 433 120 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1254
Size: 1210 44
EcoRI G'AATT_C
Cuts at: 0 397 1254
Size: 397 857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 601 1254
Size: 601 653
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1254
Size: 55 32 1167
Fragments arranged by size:
1167 55 32
HindIII A'AGCT_T
Cuts at: 0 361 1254
Size: 361 893
MslI CAynn'nnrTG
Cuts at: 0 406 752 1254
Size: 406 346 502
Fragments arranged by size:
502 406 346
MspA1I CmG'CkG
Cuts at: 0 86 1254
Size: 86 1168
NaeI GCC'GGC
Cuts at: 0 15 1254
Size: 15 1239
NgoMIV G'CCGG_C
Cuts at: 0 13 1254
Size: 13 1241
NsiI A_TGCA'T
Cuts at: 0 467 717 1254
Size: 467 250 537
Fragments arranged by size:
537 467 250
NspI r_CATG'y
Cuts at: 0 1198 1254
Size: 1198 56
PstI C_TGCA'G
Cuts at: 0 134 762 1223 1254
Size: 134 628 461 31
Fragments arranged by size:
628 461 134 31
SacII CC_GC'GG
Cuts at: 0 87 1254
Size: 87 1167
SfcI C'TryA_G
Cuts at: 0 130 355 758 815 1048 1210 1219 1254
Size: 130 225 403 57 233 162 9 35
Fragments arranged by size:
403 233 225 162 130 57 35 9
SmlI C'TyrA_G
Cuts at: 0 933 944 1254
Size: 933 11 310
Fragments arranged by size:
933 310 11
SpeI A'CTAG_T
Cuts at: 0 984 1254
Size: 984 270
SphI G_CATG'C
Cuts at: 0 1198 1254
Size: 1198 56
SspI AAT'ATT
Cuts at: 0 433 443 928 1254
Size: 433 10 485 326
Fragments arranged by size:
485 433 326 10
StuI AGG'CCT
Cuts at: 0 47 1254
Size: 47 1207
SwaI ATTT'AAAT
Cuts at: 0 518 1254
Size: 518 736
XmnI GAAnn'nnTTC
Cuts at: 0 780 1254
Size: 780 474
Enzymes that do cut:
AarI AcuI AflII AleI AlwNI ApoI BbsI BfrBI
BglII Bpu10I BpuEI BsaXI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstYI BtgI
BtgZI BtsI DraI EarI Eco57MI EcoRI FalI Hin4I
HindIII MslI MspA1I NaeI NgoMIV NsiI NspI PstI
SacII SfcI SmlI SpeI SphI SspI StuI SwaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BsrGI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI