Restriction map for
pF1KB6637
(Linear) (Six-base) MAPSORT of: KIBB6637.seq Check: 1251 from: 1 to: 1254
KIBB6637.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 463 988 990 1072 1127 1254
Size: 159 304 525 2 82 55 127
Fragments arranged by size:
525 304 159 127 82 55 2
AhdI GACnn_n'nnGTC
Cuts at: 0 915 1254
Size: 915 339
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 60 1254
Size: 46 14 1194
Fragments arranged by size:
1194 46 14
AlwNI CAG_nnn'CTG
Cuts at: 0 858 1254
Size: 858 396
ApoI r'AATT_y
Cuts at: 0 312 1254
Size: 312 942
AvaI C'yCGr_G
Cuts at: 0 1156 1254
Size: 1156 98
BamHI G'GATC_C
Cuts at: 0 75 1254
Size: 75 1179
BanI G'GyrC_C
Cuts at: 0 723 1044 1254
Size: 723 321 210
Fragments arranged by size:
723 321 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 106 914 996 1176 1254
Size: 106 808 82 180 78
Fragments arranged by size:
808 180 106 82 78
BbvCI CC'TCA_GC
Cuts at: 0 71 430 1254
Size: 71 359 824
Fragments arranged by size:
824 359 71
BclI T'GATC_A
Cuts at: 0 120 1254
Size: 120 1134
BmrI ACTGGGnnnn_n'
Cuts at: 0 226 997 1254
Size: 226 771 257
Fragments arranged by size:
771 257 226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 385 1254
Size: 322 63 869
Fragments arranged by size:
869 322 63
Bpu10I CC'TnA_GC
Cuts at: 0 71 430 1254
Size: 71 359 824
Fragments arranged by size:
824 359 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1254
Size: 551 703
BsaI GGTCTCn'nnnn_
Cuts at: 0 651 1254
Size: 651 603
BsaAI yAC'GTr
Cuts at: 0 552 699 1254
Size: 552 147 555
Fragments arranged by size:
555 552 147
BsaBI GATnn'nnATC
Cuts at: 0 125 1254
Size: 125 1129
BsaWI w'CCGG_w
Cuts at: 0 333 1254
Size: 333 921
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1197 1227 1254
Size: 1197 30 27
Fragments arranged by size:
1197 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 1054 1254
Size: 358 696 200
Fragments arranged by size:
696 358 200
BseYI C'CCAG_C
Cuts at: 0 782 1114 1254
Size: 782 332 140
Fragments arranged by size:
782 332 140
BspEI T'CCGG_A
Cuts at: 0 333 1254
Size: 333 921
BsrDI GCAATG_nn'
Cuts at: 0 263 423 816 1017 1254
Size: 263 160 393 201 237
Fragments arranged by size:
393 263 237 201 160
BsrFI r'CCGG_y
Cuts at: 0 412 1254
Size: 412 842
BssSI C'ACGA_G
Cuts at: 0 15 1254
Size: 15 1239
BstEII G'GTnAC_C
Cuts at: 0 708 1254
Size: 708 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 1254
Size: 897 357
BstYI r'GATC_y
Cuts at: 0 75 344 1254
Size: 75 269 910
Fragments arranged by size:
910 269 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 1254
Size: 225 1029
DraI TTT'AAA
Cuts at: 0 642 1254
Size: 642 612
EarI CTCTTCn'nnn_
Cuts at: 0 518 680 964 1225 1254
Size: 518 162 284 261 29
Fragments arranged by size:
518 284 261 162 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 322 385 463 988 990 1072 1127
Size: 159 163 63 78 525 2 82 55
Cuts at: 1127 1254
Size: 127
Fragments arranged by size:
525 163 159 127 82 78 63 55
2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 433 1254
Size: 70 363 821
Fragments arranged by size:
821 363 70
EcoO109I rG'GnC_Cy
Cuts at: 0 306 438 1254
Size: 306 132 816
Fragments arranged by size:
816 306 132
EcoRV GAT'ATC
Cuts at: 0 882 1254
Size: 882 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 1254
Size: 901 32 321
Fragments arranged by size:
901 321 32
HincII GTy'rAC
Cuts at: 0 507 985 1254
Size: 507 478 269
Fragments arranged by size:
507 478 269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1254
Size: 230 1024
MslI CAynn'nnrTG
Cuts at: 0 1141 1254
Size: 1141 113
MspA1I CmG'CkG
Cuts at: 0 192 405 782 1254
Size: 192 213 377 472
Fragments arranged by size:
472 377 213 192
NspI r_CATG'y
Cuts at: 0 750 1254
Size: 750 504
PasI CC'CwG_GG
Cuts at: 0 81 267 1254
Size: 81 186 987
Fragments arranged by size:
987 186 81
PfoI T'CCnGG_A
Cuts at: 0 299 359 896 1254
Size: 299 60 537 358
Fragments arranged by size:
537 358 299 60
PmlI CAC'GTG
Cuts at: 0 699 1254
Size: 699 555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1182 1254
Size: 1150 32 72
Fragments arranged by size:
1150 72 32
PvuII CAG'CTG
Cuts at: 0 192 405 782 1254
Size: 192 213 377 472
Fragments arranged by size:
472 377 213 192
SapI GCTCTTCn'nnn_
Cuts at: 0 964 1254
Size: 964 290
SexAI A'CCwGG_T
Cuts at: 0 704 1254
Size: 704 550
SfcI C'TryA_G
Cuts at: 0 245 850 1254
Size: 245 605 404
Fragments arranged by size:
605 404 245
SmlI C'TyrA_G
Cuts at: 0 566 1254
Size: 566 688
StuI AGG'CCT
Cuts at: 0 41 1254
Size: 41 1213
StyI C'CwwG_G
Cuts at: 0 275 1061 1254
Size: 275 786 193
Fragments arranged by size:
786 275 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 146 774 889 1254
Size: 146 628 115 365
Fragments arranged by size:
628 365 146 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 315 347 1220 1252 1254
Size: 315 32 873 32 2
Fragments arranged by size:
873 315 32 32 2
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApoI AvaI BamHI BanI
BbsI BbvCI BclI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BspEI BsrDI
BsrFI BssSI BstEII BstXI BstYI BtgZI DraI EarI
Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII MmeI MslI
MspA1I NspI PasI PfoI PmlI PpiI PvuII SapI
SexAI SfcI SmlI StuI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbeI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BsaHI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRI FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI