Restriction map for
pF1KB6639
(Linear) (Six-base) MAPSORT of: KIBB6639.seq Check: 972 from: 1 to: 1254
KIBB6639.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AatII G_ACGT'C
Cuts at: 0 939 999 1254
Size: 939 60 255
Fragments arranged by size:
939 255 60
AccI GT'mk_AC
Cuts at: 0 46 766 1254
Size: 46 720 488
Fragments arranged by size:
720 488 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 563 1254
Size: 335 228 691
Fragments arranged by size:
691 335 228
AfeI AGC'GCT
Cuts at: 0 688 1254
Size: 688 566
AgeI A'CCGG_T
Cuts at: 0 490 1254
Size: 490 764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1139 1254
Size: 1139 115
AlwNI CAG_nnn'CTG
Cuts at: 0 313 618 1254
Size: 313 305 636
Fragments arranged by size:
636 313 305
ApoI r'AATT_y
Cuts at: 0 721 1254
Size: 721 533
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 1254
Size: 323 931
BclI T'GATC_A
Cuts at: 0 132 1254
Size: 132 1122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 632 986 1254
Size: 341 291 354 268
Fragments arranged by size:
354 341 291 268
Bpu10I CC'TnA_GC
Cuts at: 0 444 671 1254
Size: 444 227 583
Fragments arranged by size:
583 444 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1254
Size: 447 807
BsaI GGTCTCn'nnnn_
Cuts at: 0 620 1254
Size: 620 634
BsaHI Gr'CG_yC
Cuts at: 0 936 996 1254
Size: 936 60 258
Fragments arranged by size:
936 258 60
BsaWI w'CCGG_w
Cuts at: 0 490 1254
Size: 490 764
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 327 357 503 533 1254
Size: 327 30 146 30 721
Fragments arranged by size:
721 327 146 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1254
Size: 448 806
BsrDI GCAATG_nn'
Cuts at: 0 419 1254
Size: 419 835
BsrFI r'CCGG_y
Cuts at: 0 15 490 1254
Size: 15 475 764
Fragments arranged by size:
764 475 15
BsrGI T'GTAC_A
Cuts at: 0 416 1092 1254
Size: 416 676 162
Fragments arranged by size:
676 416 162
BstZ17I GTA'TAC
Cuts at: 0 47 767 1254
Size: 47 720 487
Fragments arranged by size:
720 487 47
BtgI C'CryG_G
Cuts at: 0 237 1254
Size: 237 1017
ClaI AT'CG_AT
Cuts at: 0 806 1254
Size: 806 448
DraI TTT'AAA
Cuts at: 0 294 1254
Size: 294 960
EaeI y'GGCC_r
Cuts at: 0 226 1254
Size: 226 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 223 1254
Size: 223 1031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 341 563 632 986 1254
Size: 335 6 222 69 354 268
Fragments arranged by size:
354 335 268 222 69 6
EcoO109I rG'GnC_Cy
Cuts at: 0 832 1254
Size: 832 422
HaeII r_GCGC'y
Cuts at: 0 690 1254
Size: 690 564
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 327 359 503 535 824 856 926 958
Size: 327 32 144 32 289 32 70 32
Cuts at: 958 1254
Size: 296
Fragments arranged by size:
327 296 289 144 70 32 32 32
32
HincII GTy'rAC
Cuts at: 0 488 1254
Size: 488 766
MscI TGG'CCA
Cuts at: 0 228 1254
Size: 228 1026
MslI CAynn'nnrTG
Cuts at: 0 424 535 1254
Size: 424 111 719
Fragments arranged by size:
719 424 111
MspA1I CmG'CkG
Cuts at: 0 563 1254
Size: 563 691
NaeI GCC'GGC
Cuts at: 0 17 1254
Size: 17 1237
NcoI C'CATG_G
Cuts at: 0 237 1254
Size: 237 1017
NdeI CA'TA_TG
Cuts at: 0 213 1254
Size: 213 1041
NgoMIV G'CCGG_C
Cuts at: 0 15 1254
Size: 15 1239
NspI r_CATG'y
Cuts at: 0 1116 1254
Size: 1116 138
PflMI CCAn_nnn'nTGG
Cuts at: 0 1240 1254
Size: 1240 14
PpuMI rG'GwC_Cy
Cuts at: 0 832 1254
Size: 832 422
PvuII CAG'CTG
Cuts at: 0 563 1254
Size: 563 691
SfcI C'TryA_G
Cuts at: 0 690 1025 1254
Size: 690 335 229
Fragments arranged by size:
690 335 229
SmlI C'TyrA_G
Cuts at: 0 462 1254
Size: 462 792
SspI AAT'ATT
Cuts at: 0 298 954 1254
Size: 298 656 300
Fragments arranged by size:
656 300 298
StyI C'CwwG_G
Cuts at: 0 237 659 1254
Size: 237 422 595
Fragments arranged by size:
595 422 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 644 1254
Size: 118 526 610
Fragments arranged by size:
610 526 118
TatI w'GTAC_w
Cuts at: 0 416 1092 1254
Size: 416 676 162
Fragments arranged by size:
676 416 162
XmnI GAAnn'nnTTC
Cuts at: 0 908 1254
Size: 908 346
ZraI GAC'GTC
Cuts at: 0 937 997 1254
Size: 937 60 257
Fragments arranged by size:
937 257 60
Enzymes that do cut:
AatII AccI AcuI AfeI AgeI AlfI AlwNI ApoI
BbsI BclI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BsgI BsrDI BsrFI BsrGI BstZ17I BtgI ClaI
DraI EaeI EciI Eco57MI EcoO109I HaeII Hin4I HincII
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PflMI PpuMI PvuII SfcI SmlI SspI StyI TaqII
TatI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaAI BsaBI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
CspCI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI