(Linear) (Six-base) MAPSORT of: KIBB6640.seq  Check: 9744  from: 1  to: 1326



KIBB6640.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     578    1326 
   Size:        446     132     748

  Fragments arranged by size: 

                 748     446     132
 
AflIII A'CryG_T

Cuts at:      0     282    1326 
   Size:        282    1044
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     140    1326 
   Size:        140    1186
 
ApoI r'AATT_y

Cuts at:      0     710     719     899    1164    1326 
   Size:        710       9     180     265     162

  Fragments arranged by size: 

                 710     265     180     162       9
 
BanI G'GyrC_C

Cuts at:      0     853    1326 
   Size:        853     473
 
BanII G_rGCy'C

Cuts at:      0     312    1326 
   Size:        312    1014
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     434     515    1326 
   Size:        434      81     811

  Fragments arranged by size: 

                 811     434      81
 
BlpI GC'TnA_GC

Cuts at:      0     311    1326 
   Size:        311    1015
 
Bme1580I G_kGCm'C

Cuts at:      0    1275    1326 
   Size:       1275      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      46    1326 
   Size:         46    1280
 
BmtI G_CTAG'C

Cuts at:      0     547    1326 
   Size:        547     779
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      34    1326 
   Size:         34    1292
 
BsaAI yAC'GTr

Cuts at:      0    1270    1326 
   Size:       1270      56
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1307    1326 
   Size:       1307      19
 
BsmI GAATG_Cn'

Cuts at:      0     688    1326 
   Size:        688     638
 
Bsp1286I G_dGCh'C

Cuts at:      0     312    1275    1326 
   Size:        312     963      51

  Fragments arranged by size: 

                 963     312      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1087    1326 
   Size:       1087     239
 
BsrGI T'GTAC_A

Cuts at:      0    1014    1326 
   Size:       1014     312
 
BssHII G'CGCG_C

Cuts at:      0      21    1326 
   Size:         21    1305
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     265    1155    1326 
   Size:        265     890     171

  Fragments arranged by size: 

                 890     265     171
 
BtgI C'CryG_G

Cuts at:      0      11    1326 
   Size:         11    1315
 
BtsI GCAGTG_nn'

Cuts at:      0     524     566    1326 
   Size:        524      42     760

  Fragments arranged by size: 

                 760     524      42
 
EaeI y'GGCC_r

Cuts at:      0     270    1326 
   Size:        270    1056
 
EarI CTCTTCn'nnn_

Cuts at:      0     594     803    1326 
   Size:        594     209     523

  Fragments arranged by size: 

                 594     523     209
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     578    1326 
   Size:        446     132     748

  Fragments arranged by size: 

                 748     446     132
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     865    1326 
   Size:        865     461
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1254    1326 
   Size:       1254      72
 
EcoRI G'AATT_C

Cuts at:      0     899    1326 
   Size:        899     427
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     801    1326 
   Size:        801     525
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     505     537    1326 
   Size:        505      32     789

  Fragments arranged by size: 

                 789     505      32
 
HincII GTy'rAC

Cuts at:      0    1236    1326 
   Size:       1236      90
 
MfeI C'AATT_G

Cuts at:      0     457    1190    1326 
   Size:        457     733     136

  Fragments arranged by size: 

                 733     457     136
 
MscI TGG'CCA

Cuts at:      0     272    1326 
   Size:        272    1054
 
MslI CAynn'nnrTG

Cuts at:      0     660    1326 
   Size:        660     666
 
MspA1I CmG'CkG

Cuts at:      0      13    1326 
   Size:         13    1313
 
NheI G'CTAG_C

Cuts at:      0     543    1326 
   Size:        543     783
 
NspI r_CATG'y

Cuts at:      0     286    1326 
   Size:        286    1040
 
PasI CC'CwG_GG

Cuts at:      0     703    1326 
   Size:        703     623
 
PciI A'CATG_T

Cuts at:      0     282    1326 
   Size:        282    1044
 
PpuMI rG'GwC_Cy

Cuts at:      0    1254    1326 
   Size:       1254      72
 
PsiI TTA'TAA

Cuts at:      0     485    1326 
   Size:        485     841
 
PstI C_TGCA'G

Cuts at:      0     261    1326 
   Size:        261    1065
 
SacII CC_GC'GG

Cuts at:      0      14    1326 
   Size:         14    1312
 
SfcI C'TryA_G

Cuts at:      0     257    1326 
   Size:        257    1069
 
SpeI A'CTAG_T

Cuts at:      0    1002    1326 
   Size:       1002     324
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      48    1326 
   Size:         48    1278
 
TatI w'GTAC_w

Cuts at:      0     385     812    1014    1326 
   Size:        385     427     202     312

  Fragments arranged by size: 

                 427     385     312     202
 
XbaI T'CTAG_A

Cuts at:      0    1287    1326 
   Size:       1287      39
 
XmnI GAAnn'nnTTC

Cuts at:      0     784    1058    1326 
   Size:        784     274     268

  Fragments arranged by size: 

                 784     274     268
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI     BanI    BanII     BbsI     BlpI
 Bme1580I     BmrI     BmtI     BsaI    BsaAI    BseRI     BsmI Bsp1286I
    BspMI    BsrGI   BssHII   BstAPI     BtgI     BtsI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII     MfeI
     MscI     MslI   MspA1I     NheI     NspI     PasI     PciI    PpuMI
     PsiI     PstI    SacII     SfcI     SpeI    TaqII     TatI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI