Restriction map for
pF1KB6647
(Linear) (Six-base) MAPSORT of: KIBB6647.seq Check: 5157 from: 1 to: 1212
KIBB6647.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 350 1212
Size: 350 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 612 1212
Size: 127 485 600
Fragments arranged by size:
600 485 127
AflIII A'CryG_T
Cuts at: 0 427 1212
Size: 427 785
AlwNI CAG_nnn'CTG
Cuts at: 0 144 994 1212
Size: 144 850 218
Fragments arranged by size:
850 218 144
ApaI G_GGCC'C
Cuts at: 0 123 929 1212
Size: 123 806 283
Fragments arranged by size:
806 283 123
AvaI C'yCGr_G
Cuts at: 0 590 1212
Size: 590 622
BamHI G'GATC_C
Cuts at: 0 269 1037 1212
Size: 269 768 175
Fragments arranged by size:
768 269 175
BanI G'GyrC_C
Cuts at: 0 293 492 1212
Size: 293 199 720
Fragments arranged by size:
720 293 199
BanII G_rGCy'C
Cuts at: 0 123 554 929 1212
Size: 123 431 375 283
Fragments arranged by size:
431 375 283 123
Bme1580I G_kGCm'C
Cuts at: 0 123 830 929 1212
Size: 123 707 99 283
Fragments arranged by size:
707 283 123 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1212
Size: 724 488
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 1212
Size: 316 896
BsaWI w'CCGG_w
Cuts at: 0 290 1212
Size: 290 922
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 313 343 500 530 1212
Size: 14 30 269 30 157 30 682
Fragments arranged by size:
682 269 157 30 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 218 611 721 788 988 1121 1126 1159
Size: 218 393 110 67 200 133 5 33
Cuts at: 1159 1162 1212
Size: 3 50
Fragments arranged by size:
393 218 200 133 110 67 50 33
5 3
BseYI C'CCAG_C
Cuts at: 0 137 585 788 844 1011 1212
Size: 137 448 203 56 167 201
Fragments arranged by size:
448 203 201 167 137 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1212
Size: 1175 37
BsiHKAI G_wGCw'C
Cuts at: 0 69 1212
Size: 69 1143
Bsp1286I G_dGCh'C
Cuts at: 0 69 123 554 830 929 1212
Size: 69 54 431 276 99 283
Fragments arranged by size:
431 283 276 99 69 54
BsrDI GCAATG_nn'
Cuts at: 0 308 1212
Size: 308 904
BssSI C'ACGA_G
Cuts at: 0 227 1212
Size: 227 985
BstEII G'GTnAC_C
Cuts at: 0 579 1212
Size: 579 633
BstXI CCAn_nnnn'nTGG
Cuts at: 0 361 420 1212
Size: 361 59 792
Fragments arranged by size:
792 361 59
BstYI r'GATC_y
Cuts at: 0 269 600 1037 1212
Size: 269 331 437 175
Fragments arranged by size:
437 331 269 175
Bsu36I CC'TnA_GG
Cuts at: 0 878 1181 1212
Size: 878 303 31
Fragments arranged by size:
878 303 31
BtgI C'CryG_G
Cuts at: 0 921 1212
Size: 921 291
BtsI GCAGTG_nn'
Cuts at: 0 559 1212
Size: 559 653
DrdI GACnn_nn'nnGTC
Cuts at: 0 432 1212
Size: 432 780
EaeI y'GGCC_r
Cuts at: 0 905 1212
Size: 905 307
EarI CTCTTCn'nnn_
Cuts at: 0 256 559 1136 1212
Size: 256 303 577 76
Fragments arranged by size:
577 303 256 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 612 724 1212
Size: 127 485 112 488
Fragments arranged by size:
488 485 127 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 545 869 1184 1212
Size: 545 324 315 28
Fragments arranged by size:
545 324 315 28
EcoO109I rG'GnC_Cy
Cuts at: 0 119 120 186 208 874 926 1122 1207
Size: 119 1 66 22 666 52 196 85
Cuts at: 1207 1212
Size: 5
Fragments arranged by size:
666 196 119 85 66 52 22 5
1
FspI TGC'GCA
Cuts at: 0 661 1212
Size: 661 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 332 345 364 373 405 861 893
Size: 313 19 13 19 9 32 456 32
Cuts at: 893 1212
Size: 319
Fragments arranged by size:
456 319 313 32 32 19 19 13
9
HindIII A'AGCT_T
Cuts at: 0 176 1212
Size: 176 1036
MfeI C'AATT_G
Cuts at: 0 715 1212
Size: 715 497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 548 1151 1212
Size: 356 192 603 61
Fragments arranged by size:
603 356 192 61
MscI TGG'CCA
Cuts at: 0 907 1212
Size: 907 305
MslI CAynn'nnrTG
Cuts at: 0 476 1212
Size: 476 736
MspA1I CmG'CkG
Cuts at: 0 141 899 1212
Size: 141 758 313
Fragments arranged by size:
758 313 141
NspI r_CATG'y
Cuts at: 0 431 1212
Size: 431 781
PasI CC'CwG_GG
Cuts at: 0 198 212 1032 1212
Size: 198 14 820 180
Fragments arranged by size:
820 198 180 14
PciI A'CATG_T
Cuts at: 0 427 1212
Size: 427 785
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 720 736 1212
Size: 144 576 16 476
Fragments arranged by size:
576 476 144 16
PfoI T'CCnGG_A
Cuts at: 0 272 1212
Size: 272 940
PpuMI rG'GwC_Cy
Cuts at: 0 186 1212
Size: 186 1026
PspOMI G'GGCC_C
Cuts at: 0 119 925 1212
Size: 119 806 287
Fragments arranged by size:
806 287 119
PstI C_TGCA'G
Cuts at: 0 456 566 990 1212
Size: 456 110 424 222
Fragments arranged by size:
456 424 222 110
PvuII CAG'CTG
Cuts at: 0 899 1212
Size: 899 313
SapI GCTCTTCn'nnn_
Cuts at: 0 559 1212
Size: 559 653
SbfI CC_TGCA'GG
Cuts at: 0 456 1212
Size: 456 756
ScaI AGT'ACT
Cuts at: 0 1200 1212
Size: 1200 12
SfcI C'TryA_G
Cuts at: 0 452 562 621 894 986 1212
Size: 452 110 59 273 92 226
Fragments arranged by size:
452 273 226 110 92 59
SmaI CCC'GGG
Cuts at: 0 592 1212
Size: 592 620
StuI AGG'CCT
Cuts at: 0 638 1049 1212
Size: 638 411 163
Fragments arranged by size:
638 411 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 925 1212
Size: 18 907 287
Fragments arranged by size:
907 287 18
TatI w'GTAC_w
Cuts at: 0 1198 1212
Size: 1198 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 500 532 1212
Size: 14 32 454 32 680
Fragments arranged by size:
680 454 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 0 327 1212
Size: 327 885
XmaI C'CCGG_G
Cuts at: 0 590 1212
Size: 590 622
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI AvaI BamHI BanI
BanII Bme1580I BpmI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI
EcoO109I FspI Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SapI SbfI ScaI SfcI SmaI StuI
TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HaeII HincII HpaI KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI XbaI XcmI XhoI XmnI ZraI