Restriction map for
pF1KB6648
(Linear) (Six-base) MAPSORT of: KIBB6648.seq Check: 3886 from: 1 to: 1335
KIBB6648.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 391 943 1258 1335
Size: 182 209 552 315 77
Fragments arranged by size:
552 315 209 182 77
AflIII A'CryG_T
Cuts at: 0 545 1335
Size: 545 790
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1069 1070 1101 1102 1335
Size: 1069 1 31 1 233
Fragments arranged by size:
1069 233 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 139 1323 1335
Size: 139 1184 12
Fragments arranged by size:
1184 139 12
ApoI r'AATT_y
Cuts at: 0 168 694 704 791 929 1194 1335
Size: 168 526 10 87 138 265 141
Fragments arranged by size:
526 265 168 141 138 87 10
AseI AT'TA_AT
Cuts at: 0 634 1335
Size: 634 701
AvaI C'yCGr_G
Cuts at: 0 8 1335
Size: 8 1327
BanII G_rGCy'C
Cuts at: 0 957 1272 1335
Size: 957 315 63
Fragments arranged by size:
957 315 63
BbvCI CC'TCA_GC
Cuts at: 0 14 1335
Size: 14 1321
BmtI G_CTAG'C
Cuts at: 0 487 1335
Size: 487 848
Bpu10I CC'TnA_GC
Cuts at: 0 14 1335
Size: 14 1321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 868 1256 1335
Size: 817 51 388 79
Fragments arranged by size:
817 388 79 51
BsaBI GATnn'nnATC
Cuts at: 0 770 1335
Size: 770 565
BsaWI w'CCGG_w
Cuts at: 0 74 227 1335
Size: 74 153 1108
Fragments arranged by size:
1108 153 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 69 99 109 139 1335
Size: 69 30 10 30 1196
Fragments arranged by size:
1196 69 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1200 1335
Size: 1200 135
BseYI C'CCAG_C
Cuts at: 0 673 1259 1335
Size: 673 586 76
Fragments arranged by size:
673 586 76
BsiEI CG_ry'CG
Cuts at: 0 60 1335
Size: 60 1275
BsiHKAI G_wGCw'C
Cuts at: 0 957 1335
Size: 957 378
BsmI GAATG_Cn'
Cuts at: 0 382 1335
Size: 382 953
Bsp1286I G_dGCh'C
Cuts at: 0 957 1272 1335
Size: 957 315 63
Fragments arranged by size:
957 315 63
BspEI T'CCGG_A
Cuts at: 0 74 1335
Size: 74 1261
BspHI T'CATG_A
Cuts at: 0 1181 1335
Size: 1181 154
BsrGI T'GTAC_A
Cuts at: 0 32 245 1335
Size: 32 213 1090
Fragments arranged by size:
1090 213 32
BssSI C'ACGA_G
Cuts at: 0 507 1335
Size: 507 828
BstYI r'GATC_y
Cuts at: 0 71 276 420 1319 1335
Size: 71 205 144 899 16
Fragments arranged by size:
899 205 144 71 16
EaeI y'GGCC_r
Cuts at: 0 57 1335
Size: 57 1278
EagI C'GGCC_G
Cuts at: 0 57 1335
Size: 57 1278
EarI CTCTTCn'nnn_
Cuts at: 0 76 323 849 1335
Size: 76 247 526 486
Fragments arranged by size:
526 486 247 76
EcoICRI GAG'CTC
Cuts at: 0 955 1335
Size: 955 380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 391 943 1258 1335
Size: 182 209 552 315 77
Fragments arranged by size:
552 315 209 182 77
EcoO109I rG'GnC_Cy
Cuts at: 0 1233 1335
Size: 1233 102
EcoRI G'AATT_C
Cuts at: 0 704 1194 1335
Size: 704 490 141
Fragments arranged by size:
704 490 141
EcoRV GAT'ATC
Cuts at: 0 594 1335
Size: 594 741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 811 1335
Size: 811 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 583 615 1335
Size: 109 32 442 32 720
Fragments arranged by size:
720 442 109 32 32
HincII GTy'rAC
Cuts at: 0 359 1335
Size: 359 976
HindIII A'AGCT_T
Cuts at: 0 466 1335
Size: 466 869
MslI CAynn'nnrTG
Cuts at: 0 253 1157 1335
Size: 253 904 178
Fragments arranged by size:
904 253 178
MspA1I CmG'CkG
Cuts at: 0 62 1263 1335
Size: 62 1201 72
Fragments arranged by size:
1201 72 62
NheI G'CTAG_C
Cuts at: 0 483 1335
Size: 483 852
NspI r_CATG'y
Cuts at: 0 549 573 1332 1335
Size: 549 24 759 3
Fragments arranged by size:
759 549 24 3
PciI A'CATG_T
Cuts at: 0 545 1335
Size: 545 790
PfoI T'CCnGG_A
Cuts at: 0 443 1335
Size: 443 892
PpuMI rG'GwC_Cy
Cuts at: 0 1233 1335
Size: 1233 102
PvuII CAG'CTG
Cuts at: 0 1263 1335
Size: 1263 72
SacI G_AGCT'C
Cuts at: 0 957 1335
Size: 957 378
SfcI C'TryA_G
Cuts at: 0 1062 1335
Size: 1062 273
SmaI CCC'GGG
Cuts at: 0 10 1335
Size: 10 1325
SmlI C'TyrA_G
Cuts at: 0 796 847 1271 1335
Size: 796 51 424 64
Fragments arranged by size:
796 424 64 51
SphI G_CATG'C
Cuts at: 0 1332 1335
Size: 1332 3
SspI AAT'ATT
Cuts at: 0 262 494 1335
Size: 262 232 841
Fragments arranged by size:
841 262 232
StyI C'CwwG_G
Cuts at: 0 305 1335
Size: 305 1030
TatI w'GTAC_w
Cuts at: 0 32 245 1335
Size: 32 213 1090
Fragments arranged by size:
1090 213 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 69 101 1335
Size: 69 32 1234
Fragments arranged by size:
1234 69 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 1335
Size: 441 894
XmaI C'CCGG_G
Cuts at: 0 8 1335
Size: 8 1327
XmnI GAAnn'nnTTC
Cuts at: 0 791 1335
Size: 791 544
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApoI AseI AvaI BanII
BbvCI BmtI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrGI
BssSI BstYI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII MslI MspA1I
NheI NspI PciI PfoI PpuMI PvuII SacI SfcI
SmaI SmlI SphI SspI StyI TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BsaI BsaAI BsaHI BsgI BsiWI BsmBI BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoNI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI StuI SwaI TaqII Tth111I XbaI
XhoI ZraI