Restriction map for pF1KB6650
 (Linear) (Six-base) MAPSORT of: KIBB6650.seq  Check: 2473  from: 1  to: 1338



KIBB6650.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AatII G_ACGT'C

Cuts at:      0     113    1338 
   Size:        113    1225
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     682    1070    1338 
   Size:        496     186     388     268

  Fragments arranged by size: 

                 496     388     268     186
 
AflIII A'CryG_T

Cuts at:      0     115    1319    1338 
   Size:        115    1204      19

  Fragments arranged by size: 

                1204     115      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0     474    1338 
   Size:        474     864
 
AseI AT'TA_AT

Cuts at:      0     907    1338 
   Size:        907     431
 
AvaI C'yCGr_G

Cuts at:      0     844    1338 
   Size:        844     494
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     471     504    1338 
   Size:        471      33     834

  Fragments arranged by size: 

                 834     471      33
 
BanII G_rGCy'C

Cuts at:      0     530    1338 
   Size:        530     808
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     196     398    1319    1338 
   Size:        196     202     921      19

  Fragments arranged by size: 

                 921     202     196      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1156    1338 
   Size:       1156     182
 
BclI T'GATC_A

Cuts at:      0    1218    1338 
   Size:       1218     120
 
BglII A'GATC_T

Cuts at:      0    1280    1304    1338 
   Size:       1280      24      34

  Fragments arranged by size: 

                1280      34      24
 
BlpI GC'TnA_GC

Cuts at:      0     524    1338 
   Size:        524     814
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     481    1338 
   Size:        122     359     857

  Fragments arranged by size: 

                 857     359     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1338 
   Size:        701     637
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       1    1338 
   Size:          1    1337
 
BsaAI yAC'GTr

Cuts at:      0    1320    1338 
   Size:       1320      18
 
BsaBI GATnn'nnATC

Cuts at:      0     169     679    1338 
   Size:        169     510     659

  Fragments arranged by size: 

                 659     510     169
 
BsaHI Gr'CG_yC

Cuts at:      0     110    1338 
   Size:        110    1228
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      95     125    1338 
   Size:         95      30    1213

  Fragments arranged by size: 

                1213      95      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     559    1338 
   Size:         19     540     779

  Fragments arranged by size: 

                 779     540      19
 
BseYI C'CCAG_C

Cuts at:      0     134     704    1165    1287    1338 
   Size:        134     570     461     122      51

  Fragments arranged by size: 

                 570     461     134     122      51
 
BsiHKAI G_wGCw'C

Cuts at:      0     530    1338 
   Size:        530     808
 
Bsp1286I G_dGCh'C

Cuts at:      0     530    1338 
   Size:        530     808
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     299    1338 
   Size:        299    1039
 
BsrBI CCG'CTC

Cuts at:      0    1173    1338 
   Size:       1173     165
 
BsrDI GCAATG_nn'

Cuts at:      0     893    1338 
   Size:        893     445
 
BstYI r'GATC_y

Cuts at:      0      18    1280    1304    1338 
   Size:         18    1262      24      34

  Fragments arranged by size: 

                1262      34      24      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     422    1009    1338 
   Size:        422     587     329

  Fragments arranged by size: 

                 587     422     329
 
EcoICRI GAG'CTC

Cuts at:      0     528    1338 
   Size:        528     810
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     481     496     682    1070    1338 
   Size:        122     359      15     186     388     268

  Fragments arranged by size: 

                 388     359     268     186     122      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     295    1338 
   Size:        295    1043
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     380    1323    1338 
   Size:        380     943      15

  Fragments arranged by size: 

                 943     380      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      95     127    1338 
   Size:         95      32    1211

  Fragments arranged by size: 

                1211      95      32
 
HincII GTy'rAC

Cuts at:      0     713    1338 
   Size:        713     625
 
HindIII A'AGCT_T

Cuts at:      0     281     736    1338 
   Size:        281     455     602

  Fragments arranged by size: 

                 602     455     281
 
MfeI C'AATT_G

Cuts at:      0    1260    1338 
   Size:       1260      78
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     560    1338 
   Size:        560     778
 
MslI CAynn'nnrTG

Cuts at:      0     214    1338 
   Size:        214    1124
 
MspA1I CmG'CkG

Cuts at:      0      34     485     708     780    1338 
   Size:         34     451     223      72     558

  Fragments arranged by size: 

                 558     451     223      72      34
 
NspI r_CATG'y

Cuts at:      0     119     519    1338 
   Size:        119     400     819

  Fragments arranged by size: 

                 819     400     119
 
PciI A'CATG_T

Cuts at:      0     115    1338 
   Size:        115    1223
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1141    1338 
   Size:       1141     197
 
PfoI T'CCnGG_A

Cuts at:      0    1113    1338 
   Size:       1113     225
 
PmlI CAC'GTG

Cuts at:      0    1320    1338 
   Size:       1320      18
 
PspXI vC'TCGA_Gb

Cuts at:      0     844    1338 
   Size:        844     494
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     301     333    1338 
   Size:        301      32    1005

  Fragments arranged by size: 

                1005     301      32
 
PstI C_TGCA'G

Cuts at:      0     974    1338 
   Size:        974     364
 
PvuII CAG'CTG

Cuts at:      0      34     485     708     780    1338 
   Size:         34     451     223      72     558

  Fragments arranged by size: 

                 558     451     223      72      34
 
SacI G_AGCT'C

Cuts at:      0     530    1338 
   Size:        530     808
 
SfcI C'TryA_G

Cuts at:      0     559     970    1292    1338 
   Size:        559     411     322      46

  Fragments arranged by size: 

                 559     411     322      46
 
SmlI C'TyrA_G

Cuts at:      0     716     844    1338 
   Size:        716     128     494

  Fragments arranged by size: 

                 716     494     128
 
StyI C'CwwG_G

Cuts at:      0     296    1226    1338 
   Size:        296     930     112

  Fragments arranged by size: 

                 930     296     112
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148    1338 
   Size:        148    1190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     671     703    1338 
   Size:        671      32     635

  Fragments arranged by size: 

                 671     635      32
 
XhoI C'TCGA_G

Cuts at:      0     844    1338 
   Size:        844     494
 
ZraI GAC'GTC

Cuts at:      0     111    1338 
   Size:        111    1227
 

 Enzymes that do cut:

    AatII     AcuI   AflIII    AlwNI     AseI     AvaI     BaeI    BanII
     BbsI    BciVI     BclI    BglII     BlpI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI    BstYI     EarI  EcoICRI  Eco57MI    EcoNI
     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I
     NspI     PciI    PflMI     PfoI     PmlI    PspXI     PsrI     PstI
    PvuII     SacI     SfcI     SmlI     StyI    TaqII     TstI     XhoI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com