(Linear) (Six-base) MAPSORT of: KIBB6650.seq Check: 2473 from: 1 to: 1338 KIBB6650.orf With 182 enzymes: SgfI * October 7, 2009 14:22 .. AatII G_ACGT'C Cuts at: 0 113 1338 Size: 113 1225 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 682 1070 1338 Size: 496 186 388 268 Fragments arranged by size: 496 388 268 186 AflIII A'CryG_T Cuts at: 0 115 1319 1338 Size: 115 1204 19 Fragments arranged by size: 1204 115 19 AlwNI CAG_nnn'CTG Cuts at: 0 474 1338 Size: 474 864 AseI AT'TA_AT Cuts at: 0 907 1338 Size: 907 431 AvaI C'yCGr_G Cuts at: 0 844 1338 Size: 844 494 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 471 504 1338 Size: 471 33 834 Fragments arranged by size: 834 471 33 BanII G_rGCy'C Cuts at: 0 530 1338 Size: 530 808 BbsI GAAGACnn'nnnn_ Cuts at: 0 196 398 1319 1338 Size: 196 202 921 19 Fragments arranged by size: 921 202 196 19 BciVI GTATCCnnnnn_n' Cuts at: 0 1156 1338 Size: 1156 182 BclI T'GATC_A Cuts at: 0 1218 1338 Size: 1218 120 BglII A'GATC_T Cuts at: 0 1280 1304 1338 Size: 1280 24 34 Fragments arranged by size: 1280 34 24 BlpI GC'TnA_GC Cuts at: 0 524 1338 Size: 524 814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 481 1338 Size: 122 359 857 Fragments arranged by size: 857 359 122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 701 1338 Size: 701 637 BsaI GGTCTCn'nnnn_ Cuts at: 0 1 1338 Size: 1 1337 BsaAI yAC'GTr Cuts at: 0 1320 1338 Size: 1320 18 BsaBI GATnn'nnATC Cuts at: 0 169 679 1338 Size: 169 510 659 Fragments arranged by size: 659 510 169 BsaHI Gr'CG_yC Cuts at: 0 110 1338 Size: 110 1228 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 95 125 1338 Size: 95 30 1213 Fragments arranged by size: 1213 95 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 559 1338 Size: 19 540 779 Fragments arranged by size: 779 540 19 BseYI C'CCAG_C Cuts at: 0 134 704 1165 1287 1338 Size: 134 570 461 122 51 Fragments arranged by size: 570 461 134 122 51 BsiHKAI G_wGCw'C Cuts at: 0 530 1338 Size: 530 808 Bsp1286I G_dGCh'C Cuts at: 0 530 1338 Size: 530 808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 299 1338 Size: 299 1039 BsrBI CCG'CTC Cuts at: 0 1173 1338 Size: 1173 165 BsrDI GCAATG_nn' Cuts at: 0 893 1338 Size: 893 445 BstYI r'GATC_y Cuts at: 0 18 1280 1304 1338 Size: 18 1262 24 34 Fragments arranged by size: 1262 34 24 18 EarI CTCTTCn'nnn_ Cuts at: 0 422 1009 1338 Size: 422 587 329 Fragments arranged by size: 587 422 329 EcoICRI GAG'CTC Cuts at: 0 528 1338 Size: 528 810 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 481 496 682 1070 1338 Size: 122 359 15 186 388 268 Fragments arranged by size: 388 359 268 186 122 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 295 1338 Size: 295 1043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 380 1323 1338 Size: 380 943 15 Fragments arranged by size: 943 380 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 95 127 1338 Size: 95 32 1211 Fragments arranged by size: 1211 95 32 HincII GTy'rAC Cuts at: 0 713 1338 Size: 713 625 HindIII A'AGCT_T Cuts at: 0 281 736 1338 Size: 281 455 602 Fragments arranged by size: 602 455 281 MfeI C'AATT_G Cuts at: 0 1260 1338 Size: 1260 78 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 1338 Size: 560 778 MslI CAynn'nnrTG Cuts at: 0 214 1338 Size: 214 1124 MspA1I CmG'CkG Cuts at: 0 34 485 708 780 1338 Size: 34 451 223 72 558 Fragments arranged by size: 558 451 223 72 34 NspI r_CATG'y Cuts at: 0 119 519 1338 Size: 119 400 819 Fragments arranged by size: 819 400 119 PciI A'CATG_T Cuts at: 0 115 1338 Size: 115 1223 PflMI CCAn_nnn'nTGG Cuts at: 0 1141 1338 Size: 1141 197 PfoI T'CCnGG_A Cuts at: 0 1113 1338 Size: 1113 225 PmlI CAC'GTG Cuts at: 0 1320 1338 Size: 1320 18 PspXI vC'TCGA_Gb Cuts at: 0 844 1338 Size: 844 494 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 301 333 1338 Size: 301 32 1005 Fragments arranged by size: 1005 301 32 PstI C_TGCA'G Cuts at: 0 974 1338 Size: 974 364 PvuII CAG'CTG Cuts at: 0 34 485 708 780 1338 Size: 34 451 223 72 558 Fragments arranged by size: 558 451 223 72 34 SacI G_AGCT'C Cuts at: 0 530 1338 Size: 530 808 SfcI C'TryA_G Cuts at: 0 559 970 1292 1338 Size: 559 411 322 46 Fragments arranged by size: 559 411 322 46 SmlI C'TyrA_G Cuts at: 0 716 844 1338 Size: 716 128 494 Fragments arranged by size: 716 494 128 StyI C'CwwG_G Cuts at: 0 296 1226 1338 Size: 296 930 112 Fragments arranged by size: 930 296 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 148 1338 Size: 148 1190 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 671 703 1338 Size: 671 32 635 Fragments arranged by size: 671 635 32 XhoI C'TCGA_G Cuts at: 0 844 1338 Size: 844 494 ZraI GAC'GTC Cuts at: 0 111 1338 Size: 111 1227 Enzymes that do cut: AatII AcuI AflIII AlwNI AseI AvaI BaeI BanII BbsI BciVI BclI BglII BlpI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstYI EarI EcoICRI Eco57MI EcoNI FalI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NspI PciI PflMI PfoI PmlI PspXI PsrI PstI PvuII SacI SfcI SmlI StyI TaqII TstI XhoI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BamHI BanI BbeI BbvCI BcgI BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PpuMI PshAI PsiI PspOMI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XmaI XmnI