(Linear) (Six-base) MAPSORT of: KIBB6653.seq Check: 846 from: 1 to: 1227 KIBB6653.orf With 182 enzymes: SgfI * October 7, 2009 14:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 184 1227 Size: 184 1043 AccI GT'mk_AC Cuts at: 0 428 1227 Size: 428 799 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 533 717 1207 1227 Size: 166 367 184 490 20 Fragments arranged by size: 490 367 184 166 20 AgeI A'CCGG_T Cuts at: 0 584 1227 Size: 584 643 AleI CACnn'nnGTG Cuts at: 0 916 1227 Size: 916 311 ApoI r'AATT_y Cuts at: 0 1123 1227 Size: 1123 104 AvaI C'yCGr_G Cuts at: 0 13 598 992 1227 Size: 13 585 394 235 Fragments arranged by size: 585 394 235 13 BanI G'GyrC_C Cuts at: 0 270 928 1227 Size: 270 658 299 Fragments arranged by size: 658 299 270 BanII G_rGCy'C Cuts at: 0 43 641 1227 Size: 43 598 586 Fragments arranged by size: 598 586 43 BbsI GAAGACnn'nnnn_ Cuts at: 0 18 206 1227 Size: 18 188 1021 Fragments arranged by size: 1021 188 18 BbvCI CC'TCA_GC Cuts at: 0 316 1227 Size: 316 911 BciVI GTATCCnnnnn_n' Cuts at: 0 399 1227 Size: 399 828 BclI T'GATC_A Cuts at: 0 1215 1227 Size: 1215 12 BlpI GC'TnA_GC Cuts at: 0 835 1227 Size: 835 392 Bme1580I G_kGCm'C Cuts at: 0 275 1227 Size: 275 952 BmgBI CAC'GTC Cuts at: 0 660 1227 Size: 660 567 BmrI ACTGGGnnnn_n' Cuts at: 0 738 1068 1227 Size: 738 330 159 Fragments arranged by size: 738 330 159 Bpu10I CC'TnA_GC Cuts at: 0 316 1227 Size: 316 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 1227 Size: 865 362 BsaI GGTCTCn'nnnn_ Cuts at: 0 865 1227 Size: 865 362 BsaWI w'CCGG_w Cuts at: 0 584 1086 1227 Size: 584 502 141 Fragments arranged by size: 584 502 141 BseYI C'CCAG_C Cuts at: 0 213 324 542 793 945 1227 Size: 213 111 218 251 152 282 Fragments arranged by size: 282 251 218 213 152 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 577 1227 Size: 535 42 650 Fragments arranged by size: 650 535 42 Bsp1286I G_dGCh'C Cuts at: 0 43 275 641 1227 Size: 43 232 366 586 Fragments arranged by size: 586 366 232 43 BspEI T'CCGG_A Cuts at: 0 1086 1227 Size: 1086 141 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 184 1227 Size: 184 1043 BsrBI CCG'CTC Cuts at: 0 1084 1227 Size: 1084 143 BsrFI r'CCGG_y Cuts at: 0 308 584 1227 Size: 308 276 643 Fragments arranged by size: 643 308 276 BsrGI T'GTAC_A Cuts at: 0 815 1227 Size: 815 412 BssSI C'ACGA_G Cuts at: 0 886 1227 Size: 886 341 BtgI C'CryG_G Cuts at: 0 630 1227 Size: 630 597 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 228 1227 Size: 228 999 BtsI GCAGTG_nn' Cuts at: 0 385 829 1227 Size: 385 444 398 Fragments arranged by size: 444 398 385 EaeI y'GGCC_r Cuts at: 0 192 1227 Size: 192 1035 EarI CTCTTCn'nnn_ Cuts at: 0 461 736 1227 Size: 461 275 491 Fragments arranged by size: 491 461 275 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 533 717 1207 1227 Size: 166 367 184 490 20 Fragments arranged by size: 490 367 184 166 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 613 1227 Size: 613 614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 96 1227 Size: 96 1131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 772 804 1227 Size: 772 32 423 Fragments arranged by size: 772 423 32 MslI CAynn'nnrTG Cuts at: 0 916 1227 Size: 916 311 NcoI C'CATG_G Cuts at: 0 630 1227 Size: 630 597 NspI r_CATG'y Cuts at: 0 337 1227 Size: 337 890 PshAI GACnn'nnGTC Cuts at: 0 22 1074 1096 1227 Size: 22 1052 22 131 Fragments arranged by size: 1052 131 22 22 PspXI vC'TCGA_Gb Cuts at: 0 598 1227 Size: 598 629 PstI C_TGCA'G Cuts at: 0 305 392 1227 Size: 305 87 835 Fragments arranged by size: 835 305 87 SbfI CC_TGCA'GG Cuts at: 0 305 1227 Size: 305 922 SexAI A'CCwGG_T Cuts at: 0 782 1227 Size: 782 445 SfcI C'TryA_G Cuts at: 0 110 301 388 963 1227 Size: 110 191 87 575 264 Fragments arranged by size: 575 264 191 110 87 SmaI CCC'GGG Cuts at: 0 15 1227 Size: 15 1212 SmlI C'TyrA_G Cuts at: 0 598 880 992 1227 Size: 598 282 112 235 Fragments arranged by size: 598 282 235 112 SphI G_CATG'C Cuts at: 0 337 1227 Size: 337 890 StuI AGG'CCT Cuts at: 0 259 418 1227 Size: 259 159 809 Fragments arranged by size: 809 259 159 StyI C'CwwG_G Cuts at: 0 630 1227 Size: 630 597 TatI w'GTAC_w Cuts at: 0 62 623 746 815 1227 Size: 62 561 123 69 412 Fragments arranged by size: 561 412 123 69 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 554 586 941 973 1227 Size: 554 32 355 32 254 Fragments arranged by size: 554 355 254 32 32 XhoI C'TCGA_G Cuts at: 0 598 992 1227 Size: 598 394 235 Fragments arranged by size: 598 394 235 XmaI C'CCGG_G Cuts at: 0 13 1227 Size: 13 1214 Enzymes that do cut: AarI AccI AcuI AgeI AleI ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BsaWI BseYI BsgI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI FalI Hin4I MslI NcoI NspI PshAI PspXI PstI SbfI SexAI SfcI SmaI SmlI SphI StuI StyI TatI TstI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglI BglII BmtI BplI BpmI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XmnI ZraI