Restriction map for
pF1KB6655
(Linear) (Six-base) MAPSORT of: KIBB6655.seq Check: 8204 from: 1 to: 1269
KIBB6655.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 62 1269
Size: 62 1207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1211 1269
Size: 280 931 58
Fragments arranged by size:
931 280 58
AgeI A'CCGG_T
Cuts at: 0 1001 1269
Size: 1001 268
AleI CACnn'nnGTG
Cuts at: 0 250 1269
Size: 250 1019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 1018 1050 1269
Size: 736 32 250 32 219
Fragments arranged by size:
736 250 219 32 32
ApoI r'AATT_y
Cuts at: 0 684 1269
Size: 684 585
BciVI GTATCCnnnnn_n'
Cuts at: 0 1164 1269
Size: 1164 105
BclI T'GATC_A
Cuts at: 0 722 830 1269
Size: 722 108 439
Fragments arranged by size:
722 439 108
BfrBI ATG'CAT
Cuts at: 0 547 563 1269
Size: 547 16 706
Fragments arranged by size:
706 547 16
BglI GCCn_nnn'nGGC
Cuts at: 0 1126 1269
Size: 1126 143
BglII A'GATC_T
Cuts at: 0 872 1269
Size: 872 397
BmrI ACTGGGnnnn_n'
Cuts at: 0 64 1269
Size: 64 1205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 396 1269
Size: 60 336 873
Fragments arranged by size:
873 336 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1045 1204 1269
Size: 55 990 159 65
Fragments arranged by size:
990 159 65 55
Bpu10I CC'TnA_GC
Cuts at: 0 98 1269
Size: 98 1171
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 595 1060 1069 1269
Size: 208 387 465 9 200
Fragments arranged by size:
465 387 208 200 9
BsaAI yAC'GTr
Cuts at: 0 168 1269
Size: 168 1101
BsaHI Gr'CG_yC
Cuts at: 0 59 1269
Size: 59 1210
BsaWI w'CCGG_w
Cuts at: 0 1001 1269
Size: 1001 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 736 766 1018 1048 1269
Size: 736 30 252 30 221
Fragments arranged by size:
736 252 221 30 30
BseYI C'CCAG_C
Cuts at: 0 884 1269
Size: 884 385
BsmI GAATG_Cn'
Cuts at: 0 547 1269
Size: 547 722
BsmBI CGTCTCn'nnnn_
Cuts at: 0 423 1269
Size: 423 846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 1269
Size: 125 1144
BsrDI GCAATG_nn'
Cuts at: 0 195 1269
Size: 195 1074
BsrFI r'CCGG_y
Cuts at: 0 1001 1269
Size: 1001 268
BstEII G'GTnAC_C
Cuts at: 0 997 1269
Size: 997 272
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 1107 1269
Size: 252 855 162
Fragments arranged by size:
855 252 162
BstYI r'GATC_y
Cuts at: 0 872 986 1269
Size: 872 114 283
Fragments arranged by size:
872 283 114
Bsu36I CC'TnA_GG
Cuts at: 0 679 1269
Size: 679 590
BtgI C'CryG_G
Cuts at: 0 47 1269
Size: 47 1222
BtsI GCAGTG_nn'
Cuts at: 0 632 1269
Size: 632 637
DraIII CAC_nnn'GTG
Cuts at: 0 696 1269
Size: 696 573
EaeI y'GGCC_r
Cuts at: 0 2 74 1109 1269
Size: 2 72 1035 160
Fragments arranged by size:
1035 160 72 2
EarI CTCTTCn'nnn_
Cuts at: 0 586 1187 1208 1269
Size: 586 601 21 61
Fragments arranged by size:
601 586 61 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 280 1045 1204 1211 1269
Size: 55 225 765 159 7 58
Fragments arranged by size:
765 225 159 58 55 7
EcoO109I rG'GnC_Cy
Cuts at: 0 375 675 1269
Size: 375 300 594
Fragments arranged by size:
594 375 300
EcoRI G'AATT_C
Cuts at: 0 684 1269
Size: 684 585
EcoRV GAT'ATC
Cuts at: 0 237 777 1269
Size: 237 540 492
Fragments arranged by size:
540 492 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1269
Size: 1243 26
HincII GTy'rAC
Cuts at: 0 1047 1269
Size: 1047 222
MscI TGG'CCA
Cuts at: 0 4 76 1269
Size: 4 72 1193
Fragments arranged by size:
1193 72 4
MslI CAynn'nnrTG
Cuts at: 0 250 946 1269
Size: 250 696 323
Fragments arranged by size:
696 323 250
MspA1I CmG'CkG
Cuts at: 0 828 888 1269
Size: 828 60 381
Fragments arranged by size:
828 381 60
NcoI C'CATG_G
Cuts at: 0 47 1269
Size: 47 1222
NsiI A_TGCA'T
Cuts at: 0 549 565 1269
Size: 549 16 704
Fragments arranged by size:
704 549 16
NspI r_CATG'y
Cuts at: 0 567 956 1177 1269
Size: 567 389 221 92
Fragments arranged by size:
567 389 221 92
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 1269
Size: 696 573
PfoI T'CCnGG_A
Cuts at: 0 32 382 1269
Size: 32 350 887
Fragments arranged by size:
887 350 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 1018 1050 1269
Size: 736 32 250 32 219
Fragments arranged by size:
736 250 219 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 375 675 1269
Size: 375 300 594
Fragments arranged by size:
594 375 300
PshAI GACnn'nnGTC
Cuts at: 0 28 607 1269
Size: 28 579 662
Fragments arranged by size:
662 579 28
PvuII CAG'CTG
Cuts at: 0 888 1269
Size: 888 381
SapI GCTCTTCn'nnn_
Cuts at: 0 1208 1269
Size: 1208 61
StyI C'CwwG_G
Cuts at: 0 47 1269
Size: 47 1222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 634 1269
Size: 247 387 635
Fragments arranged by size:
635 387 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 378 410 1269
Size: 247 32 99 32 859
Fragments arranged by size:
859 247 99 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 1269
Size: 900 369
ZraI GAC'GTC
Cuts at: 0 60 1269
Size: 60 1209
Enzymes that do cut:
AatII AcuI AgeI AleI AloI ApoI BciVI BclI
BfrBI BglI BglII BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseYI BsmI BsmBI BspMI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV Hin4I
HincII MscI MslI MspA1I NcoI NsiI NspI PflMI
PfoI PpiI PpuMI PshAI PvuII SapI StyI TaqII
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BlpI Bme1580I BmgBI BmtI BpuEI BsaBI BseRI
BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI