Restriction map for
pF1KB6660
(Linear) (Six-base) MAPSORT of: KIBB6660.seq Check: 3882 from: 1 to: 1353
KIBB6660.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 82 1353
Size: 82 1271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1353
Size: 212 1141
AhdI GACnn_n'nnGTC
Cuts at: 0 205 927 1353
Size: 205 722 426
Fragments arranged by size:
722 426 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 584 1353
Size: 584 769
ApaI G_GGCC'C
Cuts at: 0 472 1353
Size: 472 881
ApoI r'AATT_y
Cuts at: 0 349 1069 1177 1345 1353
Size: 349 720 108 168 8
Fragments arranged by size:
720 349 168 108 8
AseI AT'TA_AT
Cuts at: 0 259 1353
Size: 259 1094
BanII G_rGCy'C
Cuts at: 0 472 1353
Size: 472 881
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 295 926 1353
Size: 239 56 631 427
Fragments arranged by size:
631 427 239 56
BfrBI ATG'CAT
Cuts at: 0 136 798 1353
Size: 136 662 555
Fragments arranged by size:
662 555 136
Bme1580I G_kGCm'C
Cuts at: 0 472 1353
Size: 472 881
BmrI ACTGGGnnnn_n'
Cuts at: 0 1204 1353
Size: 1204 149
Bpu10I CC'TnA_GC
Cuts at: 0 946 1353
Size: 946 407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 811 1353
Size: 212 599 542
Fragments arranged by size:
599 542 212
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 811 1353
Size: 811 542
BseYI C'CCAG_C
Cuts at: 0 465 1100 1353
Size: 465 635 253
Fragments arranged by size:
635 465 253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1353
Size: 449 904
Bsp1286I G_dGCh'C
Cuts at: 0 472 1353
Size: 472 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 1353
Size: 82 1271
BsrDI GCAATG_nn'
Cuts at: 0 156 1353
Size: 156 1197
BstBI TT'CG_AA
Cuts at: 0 1185 1259 1353
Size: 1185 74 94
Fragments arranged by size:
1185 94 74
BstXI CCAn_nnnn'nTGG
Cuts at: 0 163 1353
Size: 163 1190
Bsu36I CC'TnA_GG
Cuts at: 0 1027 1353
Size: 1027 326
BtgI C'CryG_G
Cuts at: 0 33 1353
Size: 33 1320
BtsI GCAGTG_nn'
Cuts at: 0 48 168 1040 1353
Size: 48 120 872 313
Fragments arranged by size:
872 313 120 48
EaeI y'GGCC_r
Cuts at: 0 96 887 1353
Size: 96 791 466
Fragments arranged by size:
791 466 96
EarI CTCTTCn'nnn_
Cuts at: 0 482 737 1353
Size: 482 255 616
Fragments arranged by size:
616 482 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1353
Size: 212 1141
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 1353
Size: 214 1139
EcoO109I rG'GnC_Cy
Cuts at: 0 469 950 1353
Size: 469 481 403
Fragments arranged by size:
481 469 403
EcoRI G'AATT_C
Cuts at: 0 1177 1345 1353
Size: 1177 168 8
Fragments arranged by size:
1177 168 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 322 1353
Size: 322 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 191 223 913 945 1353
Size: 191 32 690 32 408
Fragments arranged by size:
690 408 191 32 32
HindIII A'AGCT_T
Cuts at: 0 787 808 1353
Size: 787 21 545
Fragments arranged by size:
787 545 21
MfeI C'AATT_G
Cuts at: 0 140 1353
Size: 140 1213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1320 1353
Size: 1078 242 33
Fragments arranged by size:
1078 242 33
MscI TGG'CCA
Cuts at: 0 98 889 1353
Size: 98 791 464
Fragments arranged by size:
791 464 98
MslI CAynn'nnrTG
Cuts at: 0 107 161 1118 1353
Size: 107 54 957 235
Fragments arranged by size:
957 235 107 54
MspA1I CmG'CkG
Cuts at: 0 35 433 465 1005 1353
Size: 35 398 32 540 348
Fragments arranged by size:
540 398 348 35 32
NdeI CA'TA_TG
Cuts at: 0 760 1353
Size: 760 593
NsiI A_TGCA'T
Cuts at: 0 138 800 1353
Size: 138 662 553
Fragments arranged by size:
662 553 138
NspI r_CATG'y
Cuts at: 0 1089 1353
Size: 1089 264
PspOMI G'GGCC_C
Cuts at: 0 468 1353
Size: 468 885
PstI C_TGCA'G
Cuts at: 0 438 1284 1353
Size: 438 846 69
Fragments arranged by size:
846 438 69
PvuII CAG'CTG
Cuts at: 0 433 465 1353
Size: 433 32 888
Fragments arranged by size:
888 433 32
SacII CC_GC'GG
Cuts at: 0 36 1353
Size: 36 1317
SfcI C'TryA_G
Cuts at: 0 434 1280 1353
Size: 434 846 73
Fragments arranged by size:
846 434 73
SmlI C'TyrA_G
Cuts at: 0 227 790 1353
Size: 227 563 563
Fragments arranged by size:
563 563 227
StyI C'CwwG_G
Cuts at: 0 953 1353
Size: 953 400
TatI w'GTAC_w
Cuts at: 0 242 1353
Size: 242 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1196 1228 1353
Size: 1196 32 125
Fragments arranged by size:
1196 125 32
XmnI GAAnn'nnTTC
Cuts at: 0 353 1181 1353
Size: 353 828 172
Fragments arranged by size:
828 353 172
Enzymes that do cut:
AarI AcuI AhdI AlfI ApaI ApoI AseI BanII
BbsI BfrBI Bme1580I BmrI Bpu10I BpuEI BseRI BseYI
BsgI Bsp1286I BspMI BsrDI BstBI BstXI Bsu36I BtgI
BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HindIII MfeI MmeI MscI MslI MspA1I NdeI
NsiI NspI PspOMI PstI PvuII SacII SfcI SmlI
StyI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI