Restriction map for
pF1KB6665
(Linear) (Six-base) MAPSORT of: KIBB6665.seq Check: 9194 from: 1 to: 1272
KIBB6665.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 460 1192 1272
Size: 451 9 732 80
Fragments arranged by size:
732 451 80 9
AlwNI CAG_nnn'CTG
Cuts at: 0 47 627 756 1272
Size: 47 580 129 516
Fragments arranged by size:
580 516 129 47
ApaI G_GGCC'C
Cuts at: 0 183 214 1272
Size: 183 31 1058
Fragments arranged by size:
1058 183 31
AvaI C'yCGr_G
Cuts at: 0 1023 1272
Size: 1023 249
BanI G'GyrC_C
Cuts at: 0 95 1000 1202 1267 1272
Size: 95 905 202 65 5
Fragments arranged by size:
905 202 95 65 5
BanII G_rGCy'C
Cuts at: 0 183 214 958 1272
Size: 183 31 744 314
Fragments arranged by size:
744 314 183 31
BbeI G_GCGC'C
Cuts at: 0 1271 1272
Size: 1271 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 499 607 1272
Size: 499 108 665
Fragments arranged by size:
665 499 108
BglI GCCn_nnn'nGGC
Cuts at: 0 592 1232 1272
Size: 592 640 40
Fragments arranged by size:
640 592 40
BlpI GC'TnA_GC
Cuts at: 0 263 271 1144 1272
Size: 263 8 873 128
Fragments arranged by size:
873 263 128 8
Bme1580I G_kGCm'C
Cuts at: 0 98 183 214 1110 1272
Size: 98 85 31 896 162
Fragments arranged by size:
896 162 98 85 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 210 1011 1272
Size: 186 24 801 261
Fragments arranged by size:
801 261 186 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 250 307 1272
Size: 41 209 57 965
Fragments arranged by size:
965 209 57 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 1272
Size: 679 593
BsaAI yAC'GTr
Cuts at: 0 1254 1272
Size: 1254 18
BsaHI Gr'CG_yC
Cuts at: 0 1268 1272
Size: 1268 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 913 1066 1177 1198 1272
Size: 913 153 111 21 74
Fragments arranged by size:
913 153 111 74 21
BseYI C'CCAG_C
Cuts at: 0 161 366 1272
Size: 161 205 906
Fragments arranged by size:
906 205 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1272
Size: 265 1007
BsiEI CG_ry'CG
Cuts at: 0 395 1272
Size: 395 877
BsmBI CGTCTCn'nnnn_
Cuts at: 0 543 1272
Size: 543 729
Bsp1286I G_dGCh'C
Cuts at: 0 98 183 214 958 1110 1272
Size: 98 85 31 744 152 162
Fragments arranged by size:
744 162 152 98 85 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 1272
Size: 697 575
BsrBI CCG'CTC
Cuts at: 0 267 324 1272
Size: 267 57 948
Fragments arranged by size:
948 267 57
BsrFI r'CCGG_y
Cuts at: 0 98 356 1272
Size: 98 258 916
Fragments arranged by size:
916 258 98
BssHII G'CGCG_C
Cuts at: 0 135 275 330 1272
Size: 135 140 55 942
Fragments arranged by size:
942 140 135 55
BstEII G'GTnAC_C
Cuts at: 0 966 1272
Size: 966 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1254 1272
Size: 1254 18
BstYI r'GATC_y
Cuts at: 0 837 1272
Size: 837 435
Bsu36I CC'TnA_GG
Cuts at: 0 33 1272
Size: 33 1239
BtgI C'CryG_G
Cuts at: 0 200 861 1272
Size: 200 661 411
Fragments arranged by size:
661 411 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 625 1272
Size: 625 647
BtsI GCAGTG_nn'
Cuts at: 0 777 1272
Size: 777 495
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 1272
Size: 535 737
EaeI y'GGCC_r
Cuts at: 0 392 875 886 948 1125 1224 1272
Size: 392 483 11 62 177 99 48
Fragments arranged by size:
483 392 177 99 62 48 11
EagI C'GGCC_G
Cuts at: 0 392 1272
Size: 392 880
EarI CTCTTCn'nnn_
Cuts at: 0 5 427 827 1061 1272
Size: 5 422 400 234 211
Fragments arranged by size:
422 400 234 211 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 250 307 451 460 1192 1272
Size: 41 209 57 144 9 732 80
Fragments arranged by size:
732 209 144 80 57 41 9
EcoO109I rG'GnC_Cy
Cuts at: 0 111 210 211 1028 1272
Size: 111 99 1 817 244
Fragments arranged by size:
817 244 111 99 1
FspI TGC'GCA
Cuts at: 0 556 1272
Size: 556 716
HaeII r_GCGC'y
Cuts at: 0 536 708 1271 1272
Size: 536 172 563 1
Fragments arranged by size:
563 536 172 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 889 921 1042 1074 1272
Size: 594 32 263 32 121 32 198
Fragments arranged by size:
594 263 198 121 32 32 32
KasI G'GCGC_C
Cuts at: 0 1267 1272
Size: 1267 5
MscI TGG'CCA
Cuts at: 0 877 888 1127 1272
Size: 877 11 239 145
Fragments arranged by size:
877 239 145 11
MslI CAynn'nnrTG
Cuts at: 0 877 1272
Size: 877 395
MspA1I CmG'CkG
Cuts at: 0 81 122 342 552 711 1272
Size: 81 41 220 210 159 561
Fragments arranged by size:
561 220 210 159 81 41
NarI GG'CG_CC
Cuts at: 0 1268 1272
Size: 1268 4
NspI r_CATG'y
Cuts at: 0 247 1272
Size: 247 1025
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 756 884 1272
Size: 168 588 128 388
Fragments arranged by size:
588 388 168 128
PfoI T'CCnGG_A
Cuts at: 0 128 1272
Size: 128 1144
PpuMI rG'GwC_Cy
Cuts at: 0 111 1028 1272
Size: 111 917 244
Fragments arranged by size:
917 244 111
PspOMI G'GGCC_C
Cuts at: 0 179 210 1272
Size: 179 31 1062
Fragments arranged by size:
1062 179 31
PstI C_TGCA'G
Cuts at: 0 566 1272
Size: 566 706
PvuII CAG'CTG
Cuts at: 0 122 552 1272
Size: 122 430 720
Fragments arranged by size:
720 430 122
SanDI GG'GwC_CC
Cuts at: 0 1028 1272
Size: 1028 244
SexAI A'CCwGG_T
Cuts at: 0 113 1272
Size: 113 1159
SfcI C'TryA_G
Cuts at: 0 63 562 1272
Size: 63 499 710
Fragments arranged by size:
710 499 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1232 1272
Size: 1232 40
SfoI GGC'GCC
Cuts at: 0 1269 1272
Size: 1269 3
SmaI CCC'GGG
Cuts at: 0 1025 1272
Size: 1025 247
SphI G_CATG'C
Cuts at: 0 247 1272
Size: 247 1025
StuI AGG'CCT
Cuts at: 0 31 238 679 1272
Size: 31 207 441 593
Fragments arranged by size:
593 441 207 31
StyI C'CwwG_G
Cuts at: 0 587 908 1206 1272
Size: 587 321 298 66
Fragments arranged by size:
587 321 298 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 874 1272
Size: 874 398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 1272
Size: 1039 32 201
Fragments arranged by size:
1039 201 32
Tth111I GACn'n_nGTC
Cuts at: 0 115 1272
Size: 115 1157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1219 1272
Size: 1219 53
XmaI C'CCGG_G
Cuts at: 0 1023 1272
Size: 1023 249
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BglI BlpI Bme1580I BmrI BpmI BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EagI EarI Eco57MI EcoO109I FspI
HaeII Hin4I KasI MscI MslI MspA1I NarI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SexAI
SfcI SfiI SfoI SmaI SphI StuI StyI TaqII
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstZ17I ClaI CspCI
DraI DraIII EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI