Restriction map for
pF1KB6666
(Linear) (Six-base) MAPSORT of: KIBB6666.seq Check: 6839 from: 1 to: 1359
KIBB6666.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 166 185 617 1359
Size: 166 19 432 742
Fragments arranged by size:
742 432 166 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1359
Size: 905 454
AflIII A'CryG_T
Cuts at: 0 67 1359
Size: 67 1292
AlwNI CAG_nnn'CTG
Cuts at: 0 1019 1359
Size: 1019 340
ApoI r'AATT_y
Cuts at: 0 24 751 1359
Size: 24 727 608
Fragments arranged by size:
727 608 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 836 1359
Size: 215 621 523
Fragments arranged by size:
621 523 215
BfrBI ATG'CAT
Cuts at: 0 811 1359
Size: 811 548
BmrI ACTGGGnnnn_n'
Cuts at: 0 533 1359
Size: 533 826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1359
Size: 956 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 1359
Size: 375 984
BseYI C'CCAG_C
Cuts at: 0 464 759 1359
Size: 464 295 600
Fragments arranged by size:
600 464 295
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1359
Size: 1313 46
Bsp1286I G_dGCh'C
Cuts at: 0 1313 1359
Size: 1313 46
BsrBI CCG'CTC
Cuts at: 0 569 1359
Size: 569 790
BsrDI GCAATG_nn'
Cuts at: 0 149 542 1359
Size: 149 393 817
Fragments arranged by size:
817 393 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 82 862 1359
Size: 82 780 497
Fragments arranged by size:
780 497 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1111 1359
Size: 1111 248
BstYI r'GATC_y
Cuts at: 0 192 1069 1359
Size: 192 877 290
Fragments arranged by size:
877 290 192
Bsu36I CC'TnA_GG
Cuts at: 0 1346 1359
Size: 1346 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 980 1359
Size: 980 379
BtsI GCAGTG_nn'
Cuts at: 0 684 1359
Size: 684 675
ClaI AT'CG_AT
Cuts at: 0 373 629 1359
Size: 373 256 730
Fragments arranged by size:
730 373 256
EaeI y'GGCC_r
Cuts at: 0 1101 1359
Size: 1101 258
EarI CTCTTCn'nnn_
Cuts at: 0 66 658 1359
Size: 66 592 701
Fragments arranged by size:
701 592 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 956 1359
Size: 905 51 403
Fragments arranged by size:
905 403 51
EcoO109I rG'GnC_Cy
Cuts at: 0 880 1359
Size: 880 479
EcoRV GAT'ATC
Cuts at: 0 519 1359
Size: 519 840
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 184 216 280 312 1359
Size: 148 32 4 32 64 32 1047
Fragments arranged by size:
1047 148 64 32 32 32 4
HincII GTy'rAC
Cuts at: 0 444 1359
Size: 444 915
HpaI GTT'AAC
Cuts at: 0 444 1359
Size: 444 915
MfeI C'AATT_G
Cuts at: 0 1256 1359
Size: 1256 103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 294 1359
Size: 160 134 1065
Fragments arranged by size:
1065 160 134
MscI TGG'CCA
Cuts at: 0 1103 1359
Size: 1103 256
MslI CAynn'nnrTG
Cuts at: 0 1109 1359
Size: 1109 250
MspA1I CmG'CkG
Cuts at: 0 255 1359
Size: 255 1104
NdeI CA'TA_TG
Cuts at: 0 78 807 909 1359
Size: 78 729 102 450
Fragments arranged by size:
729 450 102 78
NsiI A_TGCA'T
Cuts at: 0 813 1359
Size: 813 546
NspI r_CATG'y
Cuts at: 0 71 1008 1359
Size: 71 937 351
Fragments arranged by size:
937 351 71
PciI A'CATG_T
Cuts at: 0 67 1359
Size: 67 1292
PfoI T'CCnGG_A
Cuts at: 0 334 1359
Size: 334 1025
PpuMI rG'GwC_Cy
Cuts at: 0 880 1359
Size: 880 479
PshAI GACnn'nnGTC
Cuts at: 0 729 1359
Size: 729 630
PsiI TTA'TAA
Cuts at: 0 281 1359
Size: 281 1078
PstI C_TGCA'G
Cuts at: 0 858 1359
Size: 858 501
PvuII CAG'CTG
Cuts at: 0 255 1359
Size: 255 1104
ScaI AGT'ACT
Cuts at: 0 852 1359
Size: 852 507
SfcI C'TryA_G
Cuts at: 0 196 854 1359
Size: 196 658 505
Fragments arranged by size:
658 505 196
SmlI C'TyrA_G
Cuts at: 0 390 1359
Size: 390 969
SphI G_CATG'C
Cuts at: 0 1008 1359
Size: 1008 351
StyI C'CwwG_G
Cuts at: 0 1125 1359
Size: 1125 234
TatI w'GTAC_w
Cuts at: 0 850 1359
Size: 850 509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 267 299 638 670 1359
Size: 267 32 339 32 689
Fragments arranged by size:
689 339 267 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 520 873 1359
Size: 520 353 486
Fragments arranged by size:
520 486 353
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI BbsI BfrBI BmrI
BpmI BpuEI BseYI BsiHKAI Bsp1286I BsrBI BsrDI BstAPI
BstXI BstYI Bsu36I BtgZI BtsI ClaI EaeI EarI
Eco57MI EcoO109I EcoRV Hin4I HincII HpaI MfeI MmeI
MscI MslI MspA1I NdeI NsiI NspI PciI PfoI
PpuMI PshAI PsiI PstI PvuII ScaI SfcI SmlI
SphI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
HindIII KasI KpnI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI