Restriction map for
pF1KB6668
(Linear) (Six-base) MAPSORT of: KIBB6668.seq Check: 9427 from: 1 to: 1359
KIBB6668.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 314 1333 1359
Size: 314 1019 26
Fragments arranged by size:
1019 314 26
AclI AA'CG_TT
Cuts at: 0 177 1359
Size: 177 1182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 991 1091 1359
Size: 202 789 100 268
Fragments arranged by size:
789 268 202 100
AflIII A'CryG_T
Cuts at: 0 54 1359
Size: 54 1305
AhdI GACnn_n'nnGTC
Cuts at: 0 733 1359
Size: 733 626
AlwNI CAG_nnn'CTG
Cuts at: 0 100 699 1272 1359
Size: 100 599 573 87
Fragments arranged by size:
599 573 100 87
BanI G'GyrC_C
Cuts at: 0 477 1359
Size: 477 882
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 721 1290 1359
Size: 201 520 569 69
Fragments arranged by size:
569 520 201 69
BbvCI CC'TCA_GC
Cuts at: 0 143 1289 1359
Size: 143 1146 70
Fragments arranged by size:
1146 143 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4 38 1359
Size: 4 34 1321
Fragments arranged by size:
1321 34 4
BfrBI ATG'CAT
Cuts at: 0 277 1359
Size: 277 1082
BglI GCCn_nnn'nGGC
Cuts at: 0 888 1359
Size: 888 471
BglII A'GATC_T
Cuts at: 0 485 804 1316 1359
Size: 485 319 512 43
Fragments arranged by size:
512 485 319 43
Bme1580I G_kGCm'C
Cuts at: 0 480 1258 1359
Size: 480 778 101
Fragments arranged by size:
778 480 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1359
Size: 1048 311
Bpu10I CC'TnA_GC
Cuts at: 0 143 1289 1359
Size: 143 1146 70
Fragments arranged by size:
1146 143 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1359
Size: 972 387
BsaI GGTCTCn'nnnn_
Cuts at: 0 1041 1359
Size: 1041 318
BsaHI Gr'CG_yC
Cuts at: 0 734 1228 1359
Size: 734 494 131
Fragments arranged by size:
734 494 131
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 1219 1359
Size: 367 852 140
Fragments arranged by size:
852 367 140
BseYI C'CCAG_C
Cuts at: 0 63 1359
Size: 63 1296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1329 1359
Size: 1329 30
Bsp1286I G_dGCh'C
Cuts at: 0 480 1258 1359
Size: 480 778 101
Fragments arranged by size:
778 480 101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 1359
Size: 20 1339
BstEII G'GTnAC_C
Cuts at: 0 453 1359
Size: 453 906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 1359
Size: 531 828
BstYI r'GATC_y
Cuts at: 0 485 804 1100 1316 1359
Size: 485 319 296 216 43
Fragments arranged by size:
485 319 296 216 43
BtsI GCAGTG_nn'
Cuts at: 0 399 1359
Size: 399 960
DraI TTT'AAA
Cuts at: 0 167 1359
Size: 167 1192
EaeI y'GGCC_r
Cuts at: 0 291 743 1359
Size: 291 452 616
Fragments arranged by size:
616 452 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 991 1048 1091 1359
Size: 202 789 57 43 268
Fragments arranged by size:
789 268 202 57 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 352 655 1063 1359
Size: 194 158 303 408 296
Fragments arranged by size:
408 303 296 194 158
EcoO109I rG'GnC_Cy
Cuts at: 0 437 606 1209 1359
Size: 437 169 603 150
Fragments arranged by size:
603 437 169 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 719 751 1359
Size: 73 32 614 32 608
Fragments arranged by size:
614 608 73 32 32
HincII GTy'rAC
Cuts at: 0 315 543 1359
Size: 315 228 816
Fragments arranged by size:
816 315 228
MscI TGG'CCA
Cuts at: 0 293 745 1359
Size: 293 452 614
Fragments arranged by size:
614 452 293
MslI CAynn'nnrTG
Cuts at: 0 833 1359
Size: 833 526
MspA1I CmG'CkG
Cuts at: 0 1218 1359
Size: 1218 141
NsiI A_TGCA'T
Cuts at: 0 279 1359
Size: 279 1080
NspI r_CATG'y
Cuts at: 0 58 1359
Size: 58 1301
PciI A'CATG_T
Cuts at: 0 54 1359
Size: 54 1305
PfoI T'CCnGG_A
Cuts at: 0 530 944 1064 1359
Size: 530 414 120 295
Fragments arranged by size:
530 414 295 120
PpuMI rG'GwC_Cy
Cuts at: 0 437 1209 1359
Size: 437 772 150
Fragments arranged by size:
772 437 150
PstI C_TGCA'G
Cuts at: 0 17 38 473 507 785 1359
Size: 17 21 435 34 278 574
Fragments arranged by size:
574 435 278 34 21 17
SalI G'TCGA_C
Cuts at: 0 313 1359
Size: 313 1046
SbfI CC_TGCA'GG
Cuts at: 0 17 473 1359
Size: 17 456 886
Fragments arranged by size:
886 456 17
SfcI C'TryA_G
Cuts at: 0 13 34 469 503 723 781 1359
Size: 13 21 435 34 220 58 578
Fragments arranged by size:
578 435 220 58 34 21 13
SmlI C'TyrA_G
Cuts at: 0 987 1359
Size: 987 372
StuI AGG'CCT
Cuts at: 0 775 1359
Size: 775 584
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 1045 1359
Size: 472 573 314
Fragments arranged by size:
573 472 314
Tth111I GACn'n_nGTC
Cuts at: 0 205 538 1193 1359
Size: 205 333 655 166
Fragments arranged by size:
655 333 205 166
XbaI T'CTAG_A
Cuts at: 0 1260 1359
Size: 1260 99
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1143 1239 1359
Size: 1143 96 120
Fragments arranged by size:
1143 120 96
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AlwNI BanI BbsI
BbvCI BcgI BfrBI BglI BglII Bme1580I BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI Bsp1286I BspMI
BstEII BstXI BstYI BtsI DraI EaeI Eco57MI EcoNI
EcoO109I Hin4I HincII MscI MslI MspA1I NsiI NspI
PciI PfoI PpuMI PstI SalI SbfI SfcI SmlI
StuI TaqII Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BciVI BclI BlpI
BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI TatI TstI XhoI XmaI XmnI ZraI