Restriction map for
pF1KB6669
(Linear) (Six-base) MAPSORT of: KIBB6669.seq Check: 3549 from: 1 to: 1275
KIBB6669.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 701 787 1154 1275
Size: 476 225 86 367 121
Fragments arranged by size:
476 367 225 121 86
AfeI AGC'GCT
Cuts at: 0 173 1067 1275
Size: 173 894 208
Fragments arranged by size:
894 208 173
AflIII A'CryG_T
Cuts at: 0 412 1275
Size: 412 863
AhdI GACnn_n'nnGTC
Cuts at: 0 413 1275
Size: 413 862
AlwNI CAG_nnn'CTG
Cuts at: 0 865 1275
Size: 865 410
ApaLI G'TGCA_C
Cuts at: 0 1233 1275
Size: 1233 42
ApoI r'AATT_y
Cuts at: 0 315 1077 1275
Size: 315 762 198
Fragments arranged by size:
762 315 198
AvaI C'yCGr_G
Cuts at: 0 39 1275
Size: 39 1236
BanI G'GyrC_C
Cuts at: 0 213 1275
Size: 213 1062
BanII G_rGCy'C
Cuts at: 0 923 979 1275
Size: 923 56 296
Fragments arranged by size:
923 296 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1114 1148 1275
Size: 1114 34 127
Fragments arranged by size:
1114 127 34
BglI GCCn_nnn'nGGC
Cuts at: 0 781 1275
Size: 781 494
BglII A'GATC_T
Cuts at: 0 35 602 1275
Size: 35 567 673
Fragments arranged by size:
673 567 35
Bme1580I G_kGCm'C
Cuts at: 0 1237 1275
Size: 1237 38
BmgBI CAC'GTC
Cuts at: 0 1232 1275
Size: 1232 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 458 1275
Size: 458 817
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 783 1275
Size: 783 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1275
Size: 755 520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1275
Size: 745 530
BsaI GGTCTCn'nnnn_
Cuts at: 0 1191 1275
Size: 1191 84
BsaAI yAC'GTr
Cuts at: 0 941 1275
Size: 941 334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 1275
Size: 250 1025
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1194 1275
Size: 1082 112 81
Fragments arranged by size:
1082 112 81
BsiHKAI G_wGCw'C
Cuts at: 0 29 901 958 979 1237 1275
Size: 29 872 57 21 258 38
Fragments arranged by size:
872 258 57 38 29 21
BsmI GAATG_Cn'
Cuts at: 0 1029 1275
Size: 1029 246
Bsp1286I G_dGCh'C
Cuts at: 0 29 901 923 958 979 1237 1275
Size: 29 872 22 35 21 258 38
Fragments arranged by size:
872 258 38 35 29 22 21
BsrDI GCAATG_nn'
Cuts at: 0 558 1275
Size: 558 717
BsrGI T'GTAC_A
Cuts at: 0 105 1275
Size: 105 1170
BstYI r'GATC_y
Cuts at: 0 35 602 1275
Size: 35 567 673
Fragments arranged by size:
673 567 35
BtsI GCAGTG_nn'
Cuts at: 0 27 1206 1275
Size: 27 1179 69
Fragments arranged by size:
1179 69 27
DraI TTT'AAA
Cuts at: 0 321 1275
Size: 321 954
DraIII CAC_nnn'GTG
Cuts at: 0 229 1275
Size: 229 1046
EarI CTCTTCn'nnn_
Cuts at: 0 614 1275
Size: 614 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1002 1275
Size: 1002 273
EcoICRI GAG'CTC
Cuts at: 0 977 1275
Size: 977 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 701 755 787 1154 1275
Size: 476 225 54 32 367 121
Fragments arranged by size:
476 367 225 121 54 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 914 1275
Size: 914 361
EcoO109I rG'GnC_Cy
Cuts at: 0 482 1275
Size: 482 793
EcoRI G'AATT_C
Cuts at: 0 1077 1275
Size: 1077 198
HaeII r_GCGC'y
Cuts at: 0 175 1069 1275
Size: 175 894 206
Fragments arranged by size:
894 206 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 399 431 1275
Size: 399 32 844
Fragments arranged by size:
844 399 32
HindIII A'AGCT_T
Cuts at: 0 1192 1275
Size: 1192 83
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1275
Size: 51 1224
MslI CAynn'nnrTG
Cuts at: 0 868 1275
Size: 868 407
MspA1I CmG'CkG
Cuts at: 0 669 879 1275
Size: 669 210 396
Fragments arranged by size:
669 396 210
NspI r_CATG'y
Cuts at: 0 416 1275
Size: 416 859
PciI A'CATG_T
Cuts at: 0 412 1275
Size: 412 863
PfoI T'CCnGG_A
Cuts at: 0 755 1275
Size: 755 520
PpuMI rG'GwC_Cy
Cuts at: 0 482 1275
Size: 482 793
PsiI TTA'TAA
Cuts at: 0 304 1275
Size: 304 971
PstI C_TGCA'G
Cuts at: 0 479 1275
Size: 479 796
PvuII CAG'CTG
Cuts at: 0 669 1275
Size: 669 606
SacI G_AGCT'C
Cuts at: 0 979 1275
Size: 979 296
SfcI C'TryA_G
Cuts at: 0 475 486 1275
Size: 475 11 789
Fragments arranged by size:
789 475 11
SmlI C'TyrA_G
Cuts at: 0 724 1275
Size: 724 551
SspI AAT'ATT
Cuts at: 0 655 1275
Size: 655 620
StyI C'CwwG_G
Cuts at: 0 980 1275
Size: 980 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 855 941 1275
Size: 855 86 334
Fragments arranged by size:
855 334 86
TatI w'GTAC_w
Cuts at: 0 105 1275
Size: 105 1170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1227 1259 1275
Size: 1227 32 16
Fragments arranged by size:
1227 32 16
XmnI GAAnn'nnTTC
Cuts at: 0 800 1275
Size: 800 475
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlwNI ApaLI ApoI AvaI
BanI BanII BcgI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BseRI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BsrGI BstYI BtsI DraI DraIII
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII MmeI MslI MspA1I NspI PciI PfoI
PpuMI PsiI PstI PvuII SacI SfcI SmlI SspI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BlpI
BmtI Bpu10I BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DrdI EaeI EagI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI