Restriction map for
pF1KB6672
(Linear) (Six-base) MAPSORT of: KIBB6672.seq Check: 4487 from: 1 to: 1362
KIBB6672.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1362
Size: 302 1060
AflII C'TTAA_G
Cuts at: 0 22 1362
Size: 22 1340
AlwNI CAG_nnn'CTG
Cuts at: 0 586 1212 1362
Size: 586 626 150
Fragments arranged by size:
626 586 150
ApoI r'AATT_y
Cuts at: 0 161 535 557 589 1362
Size: 161 374 22 32 773
Fragments arranged by size:
773 374 161 32 22
AseI AT'TA_AT
Cuts at: 0 782 1362
Size: 782 580
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 876 1362
Size: 129 747 486
Fragments arranged by size:
747 486 129
BciVI GTATCCnnnnn_n'
Cuts at: 0 337 377 1120 1362
Size: 337 40 743 242
Fragments arranged by size:
743 337 242 40
BfrBI ATG'CAT
Cuts at: 0 3 1362
Size: 3 1359
BglII A'GATC_T
Cuts at: 0 690 1362
Size: 690 672
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 437 1362
Size: 437 925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 90 990 1362
Size: 66 24 900 372
Fragments arranged by size:
900 372 66 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 1362
Size: 316 1046
BsaAI yAC'GTr
Cuts at: 0 1239 1362
Size: 1239 123
BsaBI GATnn'nnATC
Cuts at: 0 689 1362
Size: 689 673
BsaWI w'CCGG_w
Cuts at: 0 593 608 1362
Size: 593 15 754
Fragments arranged by size:
754 593 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 560 590 1362
Size: 560 30 772
Fragments arranged by size:
772 560 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 389 410 993 1362
Size: 389 21 583 369
Fragments arranged by size:
583 389 369 21
BseYI C'CCAG_C
Cuts at: 0 53 348 1362
Size: 53 295 1014
Fragments arranged by size:
1014 295 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 836 1362
Size: 212 624 526
Fragments arranged by size:
624 526 212
BsiHKAI G_wGCw'C
Cuts at: 0 1260 1362
Size: 1260 102
BsmI GAATG_Cn'
Cuts at: 0 1188 1362
Size: 1188 174
Bsp1286I G_dGCh'C
Cuts at: 0 1260 1362
Size: 1260 102
BspEI T'CCGG_A
Cuts at: 0 593 1362
Size: 593 769
BspHI T'CATG_A
Cuts at: 0 470 1362
Size: 470 892
BstYI r'GATC_y
Cuts at: 0 246 690 1089 1362
Size: 246 444 399 273
Fragments arranged by size:
444 399 273 246
BtsI GCAGTG_nn'
Cuts at: 0 581 1362
Size: 581 781
EarI CTCTTCn'nnn_
Cuts at: 0 278 314 1362
Size: 278 36 1048
Fragments arranged by size:
1048 278 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 90 302 990 1362
Size: 66 24 212 688 372
Fragments arranged by size:
688 372 212 66 24
EcoO109I rG'GnC_Cy
Cuts at: 0 877 1362
Size: 877 485
EcoRI G'AATT_C
Cuts at: 0 589 1362
Size: 589 773
EcoRV GAT'ATC
Cuts at: 0 753 1362
Size: 753 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 384 416 753 785 824 856 1288 1320
Size: 384 32 337 32 39 32 432 32
Cuts at: 1320 1362
Size: 42
Fragments arranged by size:
432 384 337 42 39 32 32 32
32
HincII GTy'rAC
Cuts at: 0 483 1362
Size: 483 879
HindIII A'AGCT_T
Cuts at: 0 722 1362
Size: 722 640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1002 1285 1362
Size: 269 733 283 77
Fragments arranged by size:
733 283 269 77
MslI CAynn'nnrTG
Cuts at: 0 469 1181 1362
Size: 469 712 181
Fragments arranged by size:
712 469 181
MspA1I CmG'CkG
Cuts at: 0 586 1362
Size: 586 776
NdeI CA'TA_TG
Cuts at: 0 1178 1362
Size: 1178 184
NsiI A_TGCA'T
Cuts at: 0 5 1362
Size: 5 1357
PfoI T'CCnGG_A
Cuts at: 0 1085 1362
Size: 1085 277
PpuMI rG'GwC_Cy
Cuts at: 0 877 1362
Size: 877 485
PshAI GACnn'nnGTC
Cuts at: 0 882 1362
Size: 882 480
PsiI TTA'TAA
Cuts at: 0 1031 1362
Size: 1031 331
PvuII CAG'CTG
Cuts at: 0 586 1362
Size: 586 776
ScaI AGT'ACT
Cuts at: 0 295 1362
Size: 295 1067
SfcI C'TryA_G
Cuts at: 0 1199 1362
Size: 1199 163
SmlI C'TyrA_G
Cuts at: 0 22 1362
Size: 22 1340
StyI C'CwwG_G
Cuts at: 0 311 1362
Size: 311 1051
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 765 1362
Size: 424 341 597
Fragments arranged by size:
597 424 341
TatI w'GTAC_w
Cuts at: 0 293 1289 1362
Size: 293 996 73
Fragments arranged by size:
996 293 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 431 560 592 1362
Size: 399 32 129 32 770
Fragments arranged by size:
770 399 129 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 466 1362
Size: 466 896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 562 1362
Size: 562 800
Enzymes that do cut:
AcuI AflII AlwNI ApoI AseI BbsI BciVI BfrBI
BglII BplI BpmI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BstYI BtsI EarI Eco57MI EcoO109I EcoRI EcoRV Hin4I
HincII HindIII MmeI MslI MspA1I NdeI NsiI PfoI
PpuMI PshAI PsiI PvuII ScaI SfcI SmlI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI
Bpu10I BpuEI BsaHI BsiEI BsiWI BsmBI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI XmnI ZraI