Restriction map for
pF1KB6676
(Linear) (Six-base) MAPSORT of: KIBB6676.seq Check: 9917 from: 1 to: 1362
KIBB6676.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1362
Size: 374 988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1111 1362
Size: 1111 251
AlwNI CAG_nnn'CTG
Cuts at: 0 15 78 1362
Size: 15 63 1284
Fragments arranged by size:
1284 63 15
ApaI G_GGCC'C
Cuts at: 0 306 792 1362
Size: 306 486 570
Fragments arranged by size:
570 486 306
ApoI r'AATT_y
Cuts at: 0 358 1049 1362
Size: 358 691 313
Fragments arranged by size:
691 358 313
AvaI C'yCGr_G
Cuts at: 0 784 1362
Size: 784 578
BanII G_rGCy'C
Cuts at: 0 56 306 338 792 1304 1362
Size: 56 250 32 454 512 58
Fragments arranged by size:
512 454 250 58 56 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 689 770 1362
Size: 689 81 592
Fragments arranged by size:
689 592 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1177 1211 1362
Size: 1177 34 151
Fragments arranged by size:
1177 151 34
BfrBI ATG'CAT
Cuts at: 0 583 1362
Size: 583 779
BglI GCCn_nnn'nGGC
Cuts at: 0 1246 1362
Size: 1246 116
BlpI GC'TnA_GC
Cuts at: 0 55 1362
Size: 55 1307
Bme1580I G_kGCm'C
Cuts at: 0 306 792 1362
Size: 306 486 570
Fragments arranged by size:
570 486 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 504 1362
Size: 484 20 858
Fragments arranged by size:
858 484 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1362
Size: 1045 317
BsaBI GATnn'nnATC
Cuts at: 0 38 1362
Size: 38 1324
BsaHI Gr'CG_yC
Cuts at: 0 1202 1362
Size: 1202 160
BsaWI w'CCGG_w
Cuts at: 0 408 968 1362
Size: 408 560 394
Fragments arranged by size:
560 408 394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 997 1362
Size: 997 365
BsiHKAI G_wGCw'C
Cuts at: 0 56 278 1090 1304 1362
Size: 56 222 812 214 58
Fragments arranged by size:
812 222 214 58 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 729 1362
Size: 729 633
Bsp1286I G_dGCh'C
Cuts at: 0 56 278 306 338 792 1090 1304 1362
Size: 56 222 28 32 454 298 214 58
Fragments arranged by size:
454 298 222 214 58 56 32 28
BspEI T'CCGG_A
Cuts at: 0 408 1362
Size: 408 954
BsrBI CCG'CTC
Cuts at: 0 1003 1362
Size: 1003 359
BsrGI T'GTAC_A
Cuts at: 0 26 1362
Size: 26 1336
BssSI C'ACGA_G
Cuts at: 0 758 1362
Size: 758 604
BstYI r'GATC_y
Cuts at: 0 33 74 104 1362
Size: 33 41 30 1258
Fragments arranged by size:
1258 41 33 30
BtgI C'CryG_G
Cuts at: 0 1044 1362
Size: 1044 318
ClaI AT'CG_AT
Cuts at: 0 1187 1362
Size: 1187 175
DraI TTT'AAA
Cuts at: 0 978 1362
Size: 978 384
EarI CTCTTCn'nnn_
Cuts at: 0 326 494 641 727 795 1362
Size: 326 168 147 86 68 567
Fragments arranged by size:
567 326 168 147 86 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1264 1362
Size: 1264 98
EcoICRI GAG'CTC
Cuts at: 0 54 1302 1362
Size: 54 1248 60
Fragments arranged by size:
1248 60 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 484 504 1362
Size: 374 110 20 858
Fragments arranged by size:
858 374 110 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1362
Size: 1294 68
EcoO109I rG'GnC_Cy
Cuts at: 0 302 788 1362
Size: 302 486 574
Fragments arranged by size:
574 486 302
EcoRI G'AATT_C
Cuts at: 0 358 1049 1362
Size: 358 691 313
Fragments arranged by size:
691 358 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 1362
Size: 457 32 873
Fragments arranged by size:
873 457 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1139 1362
Size: 132 1007 223
Fragments arranged by size:
1007 223 132
MslI CAynn'nnrTG
Cuts at: 0 1093 1362
Size: 1093 269
MspA1I CmG'CkG
Cuts at: 0 724 1335 1362
Size: 724 611 27
Fragments arranged by size:
724 611 27
NcoI C'CATG_G
Cuts at: 0 1044 1362
Size: 1044 318
NdeI CA'TA_TG
Cuts at: 0 579 1362
Size: 579 783
NsiI A_TGCA'T
Cuts at: 0 585 1362
Size: 585 777
NspI r_CATG'y
Cuts at: 0 1098 1240 1362
Size: 1098 142 122
Fragments arranged by size:
1098 142 122
PasI CC'CwG_GG
Cuts at: 0 1290 1362
Size: 1290 72
PspOMI G'GGCC_C
Cuts at: 0 302 788 1362
Size: 302 486 574
Fragments arranged by size:
574 486 302
PstI C_TGCA'G
Cuts at: 0 404 1200 1362
Size: 404 796 162
Fragments arranged by size:
796 404 162
PvuII CAG'CTG
Cuts at: 0 724 1335 1362
Size: 724 611 27
Fragments arranged by size:
724 611 27
SacI G_AGCT'C
Cuts at: 0 56 1304 1362
Size: 56 1248 58
Fragments arranged by size:
1248 58 56
SapI GCTCTTCn'nnn_
Cuts at: 0 326 1362
Size: 326 1036
SfcI C'TryA_G
Cuts at: 0 400 1196 1206 1231 1362
Size: 400 796 10 25 131
Fragments arranged by size:
796 400 131 25 10
SmlI C'TyrA_G
Cuts at: 0 1060 1362
Size: 1060 302
SphI G_CATG'C
Cuts at: 0 1098 1240 1362
Size: 1098 142 122
Fragments arranged by size:
1098 142 122
SspI AAT'ATT
Cuts at: 0 537 1362
Size: 537 825
StyI C'CwwG_G
Cuts at: 0 743 1044 1244 1362
Size: 743 301 200 118
Fragments arranged by size:
743 301 200 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 882 1362
Size: 225 657 480
Fragments arranged by size:
657 480 225
TatI w'GTAC_w
Cuts at: 0 26 1362
Size: 26 1336
Tth111I GACn'n_nGTC
Cuts at: 0 939 1362
Size: 939 423
XbaI T'CTAG_A
Cuts at: 0 255 1362
Size: 255 1107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1287 1362
Size: 1287 75
XmnI GAAnn'nnTTC
Cuts at: 0 1079 1362
Size: 1079 283
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApoI AvaI BanII BbsI
BcgI BfrBI BglI BlpI Bme1580I BpmI BpuEI BsaBI
BsaHI BsaWI BseRI BsiHKAI BsmBI Bsp1286I BspEI BsrBI
BsrGI BssSI BstYI BtgI ClaI DraI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I MmeI MslI
MspA1I NcoI NdeI NsiI NspI PasI PspOMI PstI
PvuII SacI SapI SfcI SmlI SphI SspI StyI
TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaXI BseYI BsgI BsiEI BsiWI BsmI BspHI BspMI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TstI XhoI XmaI ZraI