(Linear) (Six-base) MAPSORT of: KIBB6677.seq  Check: 7129  from: 1  to: 1281



KIBB6677.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

Acc65I G'GTAC_C

Cuts at:      0     678    1281 
   Size:        678     603
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     677    1281 
   Size:        212     465     604

  Fragments arranged by size: 

                 604     465     212
 
AflII C'TTAA_G

Cuts at:      0     892    1281 
   Size:        892     389
 
ApaLI G'TGCA_C

Cuts at:      0     807    1281 
   Size:        807     474
 
ApoI r'AATT_y

Cuts at:      0     736    1080    1123    1281 
   Size:        736     344      43     158

  Fragments arranged by size: 

                 736     344     158      43
 
BamHI G'GATC_C

Cuts at:      0     629    1281 
   Size:        629     652
 
BanI G'GyrC_C

Cuts at:      0     678    1281 
   Size:        678     603
 
BanII G_rGCy'C

Cuts at:      0     134    1281 
   Size:        134    1147
 
BclI T'GATC_A

Cuts at:      0     444    1281 
   Size:        444     837
 
BlpI GC'TnA_GC

Cuts at:      0     133    1281 
   Size:        133    1148
 
Bme1580I G_kGCm'C

Cuts at:      0     811    1281 
   Size:        811     470
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    1281 
   Size:        588     693
 
Bpu10I CC'TnA_GC

Cuts at:      0     304    1281 
   Size:        304     977
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     888    1281 
   Size:        888     393
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     368    1281 
   Size:        368     913
 
BsaWI w'CCGG_w

Cuts at:      0    1253    1281 
   Size:       1253      28
 
BseYI C'CCAG_C

Cuts at:      0     178     427    1281 
   Size:        178     249     854

  Fragments arranged by size: 

                 854     249     178
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     811    1281 
   Size:        134     677     470

  Fragments arranged by size: 

                 677     470     134
 
BsmI GAATG_Cn'

Cuts at:      0     297    1281 
   Size:        297     984
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     811    1281 
   Size:        134     677     470

  Fragments arranged by size: 

                 677     470     134
 
BspHI T'CATG_A

Cuts at:      0     830    1281 
   Size:        830     451
 
BsrDI GCAATG_nn'

Cuts at:      0     189    1281 
   Size:        189    1092
 
BstEII G'GTnAC_C

Cuts at:      0     144    1281 
   Size:        144    1137
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168    1281 
   Size:        168    1113
 
BstYI r'GATC_y

Cuts at:      0     629    1281 
   Size:        629     652
 
Bsu36I CC'TnA_GG

Cuts at:      0      16    1281 
   Size:         16    1265
 
BtsI GCAGTG_nn'

Cuts at:      0     809    1281 
   Size:        809     472
 
DraI TTT'AAA

Cuts at:      0    1128    1281 
   Size:       1128     153
 
EaeI y'GGCC_r

Cuts at:      0     647    1281 
   Size:        647     634
 
EarI CTCTTCn'nnn_

Cuts at:      0     781     968    1281 
   Size:        781     187     313

  Fragments arranged by size: 

                 781     313     187
 
EcoICRI GAG'CTC

Cuts at:      0     132    1281 
   Size:        132    1149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     588     677    1281 
   Size:        212     376      89     604

  Fragments arranged by size: 

                 604     376     212      89
 
EcoRI G'AATT_C

Cuts at:      0    1080    1281 
   Size:       1080     201
 
EcoRV GAT'ATC

Cuts at:      0     257    1281 
   Size:        257    1024
 
HaeII r_GCGC'y

Cuts at:      0     435    1281 
   Size:        435     846
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      38      70     345     365     377     397    1281 
   Size:         38      32     275      20      12      20     884

  Fragments arranged by size: 

                 884     275      38      32      20      20      12
 
HincII GTy'rAC

Cuts at:      0     543    1281 
   Size:        543     738
 
KpnI G_GTAC'C

Cuts at:      0     682    1281 
   Size:        682     599
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      24    1281 
   Size:         24    1257
 
MscI TGG'CCA

Cuts at:      0     649    1281 
   Size:        649     632
 
MslI CAynn'nnrTG

Cuts at:      0     101     829    1281 
   Size:        101     728     452

  Fragments arranged by size: 

                 728     452     101
 
PfoI T'CCnGG_A

Cuts at:      0     565    1281 
   Size:        565     716
 
PshAI GACnn'nnGTC

Cuts at:      0    1189    1281 
   Size:       1189      92
 
SacI G_AGCT'C

Cuts at:      0     134    1281 
   Size:        134    1147
 
SapI GCTCTTCn'nnn_

Cuts at:      0     781    1281 
   Size:        781     500
 
SmlI C'TyrA_G

Cuts at:      0     867     892    1281 
   Size:        867      25     389

  Fragments arranged by size: 

                 867     389      25
 
StyI C'CwwG_G

Cuts at:      0     369     800    1281 
   Size:        369     431     481

  Fragments arranged by size: 

                 481     431     369
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1179    1281 
   Size:       1179     102
 
TatI w'GTAC_w

Cuts at:      0     262    1281 
   Size:        262    1019
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII    ApaLI     ApoI    BamHI     BanI    BanII
     BclI     BlpI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaWI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI   BstEII    BstXI
    BstYI   Bsu36I     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI
    EcoRI    EcoRV    HaeII    Hin4I   HincII     KpnI     MmeI     MscI
     MslI     PfoI    PshAI     SacI     SapI     SmlI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI