Restriction map for
pF1KB6678
(Linear) (Six-base) MAPSORT of: KIBB6678.seq Check: 4464 from: 1 to: 1308
KIBB6678.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 66 337 1138 1308
Size: 54 12 271 801 170
Fragments arranged by size:
801 271 170 54 12
AflIII A'CryG_T
Cuts at: 0 1001 1308
Size: 1001 307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 950 982 1308
Size: 950 32 326
Fragments arranged by size:
950 326 32
AlwNI CAG_nnn'CTG
Cuts at: 0 186 421 1308
Size: 186 235 887
Fragments arranged by size:
887 235 186
ApaI G_GGCC'C
Cuts at: 0 49 1308
Size: 49 1259
AvaI C'yCGr_G
Cuts at: 0 42 48 93 1308
Size: 42 6 45 1215
Fragments arranged by size:
1215 45 42 6
BamHI G'GATC_C
Cuts at: 0 507 1308
Size: 507 801
BanII G_rGCy'C
Cuts at: 0 49 100 1308
Size: 49 51 1208
Fragments arranged by size:
1208 51 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 343 1308
Size: 343 965
BbvCI CC'TCA_GC
Cuts at: 0 386 1308
Size: 386 922
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1308
Size: 34 1274
Bme1580I G_kGCm'C
Cuts at: 0 49 1308
Size: 49 1259
BmgBI CAC'GTC
Cuts at: 0 1087 1308
Size: 1087 221
BmrI ACTGGGnnnn_n'
Cuts at: 0 1195 1308
Size: 1195 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 1308
Size: 84 1224
Bpu10I CC'TnA_GC
Cuts at: 0 386 1308
Size: 386 922
BsaI GGTCTCn'nnnn_
Cuts at: 0 1027 1106 1308
Size: 1027 79 202
Fragments arranged by size:
1027 202 79
BsaBI GATnn'nnATC
Cuts at: 0 430 1308
Size: 430 878
BsaHI Gr'CG_yC
Cuts at: 0 109 711 1308
Size: 109 602 597
Fragments arranged by size:
602 597 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 950 980 1308
Size: 82 30 838 30 328
Fragments arranged by size:
838 328 82 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 971 1308
Size: 971 337
BseYI C'CCAG_C
Cuts at: 0 918 1267 1308
Size: 918 349 41
Fragments arranged by size:
918 349 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1308
Size: 998 310
BsmI GAATG_Cn'
Cuts at: 0 210 852 1308
Size: 210 642 456
Fragments arranged by size:
642 456 210
Bsp1286I G_dGCh'C
Cuts at: 0 49 100 1308
Size: 49 51 1208
Fragments arranged by size:
1208 51 49
BsrBI CCG'CTC
Cuts at: 0 168 1308
Size: 168 1140
BsrDI GCAATG_nn'
Cuts at: 0 675 857 1308
Size: 675 182 451
Fragments arranged by size:
675 451 182
BsrFI r'CCGG_y
Cuts at: 0 37 64 1308
Size: 37 27 1244
Fragments arranged by size:
1244 37 27
BssHII G'CGCG_C
Cuts at: 0 57 148 1308
Size: 57 91 1160
Fragments arranged by size:
1160 91 57
BssSI C'ACGA_G
Cuts at: 0 1025 1243 1308
Size: 1025 218 65
Fragments arranged by size:
1025 218 65
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 1308
Size: 246 1062
BstYI r'GATC_y
Cuts at: 0 507 1308
Size: 507 801
BtsI GCAGTG_nn'
Cuts at: 0 1052 1308
Size: 1052 256
ClaI AT'CG_AT
Cuts at: 0 546 1308
Size: 546 762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 776 811 1308
Size: 776 35 497
Fragments arranged by size:
776 497 35
DraIII CAC_nnn'GTG
Cuts at: 0 828 1308
Size: 828 480
DrdI GACnn_nn'nnGTC
Cuts at: 0 1261 1308
Size: 1261 47
EaeI y'GGCC_r
Cuts at: 0 35 1308
Size: 35 1273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 66 84 337 1138 1308
Size: 54 12 18 253 801 170
Fragments arranged by size:
801 253 170 54 18 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 513 1308
Size: 513 795
FseI GG_CCGG'CC
Cuts at: 0 41 1308
Size: 41 1267
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 181 213 1308
Size: 82 32 67 32 1095
Fragments arranged by size:
1095 82 67 32 32
HincII GTy'rAC
Cuts at: 0 1178 1308
Size: 1178 130
MfeI C'AATT_G
Cuts at: 0 322 1308
Size: 322 986
MslI CAynn'nnrTG
Cuts at: 0 449 1308
Size: 449 859
NaeI GCC'GGC
Cuts at: 0 39 66 1308
Size: 39 27 1242
Fragments arranged by size:
1242 39 27
NgoMIV G'CCGG_C
Cuts at: 0 37 64 1308
Size: 37 27 1244
Fragments arranged by size:
1244 37 27
NspI r_CATG'y
Cuts at: 0 1005 1308
Size: 1005 303
PasI CC'CwG_GG
Cuts at: 0 471 1308
Size: 471 837
PciI A'CATG_T
Cuts at: 0 1001 1308
Size: 1001 307
PfoI T'CCnGG_A
Cuts at: 0 185 1308
Size: 185 1123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 950 982 1308
Size: 950 32 326
Fragments arranged by size:
950 326 32
PspOMI G'GGCC_C
Cuts at: 0 45 1308
Size: 45 1263
ScaI AGT'ACT
Cuts at: 0 535 1308
Size: 535 773
SmaI CCC'GGG
Cuts at: 0 44 50 1308
Size: 44 6 1258
Fragments arranged by size:
1258 44 6
SrfI GCCC'GGGC
Cuts at: 0 44 1308
Size: 44 1264
StyI C'CwwG_G
Cuts at: 0 32 1107 1308
Size: 32 1075 201
Fragments arranged by size:
1075 201 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 968 1308
Size: 968 340
TatI w'GTAC_w
Cuts at: 0 533 672 1308
Size: 533 139 636
Fragments arranged by size:
636 533 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1088 1120 1308
Size: 1088 32 188
Fragments arranged by size:
1088 188 32
XmaI C'CCGG_G
Cuts at: 0 42 48 1308
Size: 42 6 1260
Fragments arranged by size:
1260 42 6
XmnI GAAnn'nnTTC
Cuts at: 0 181 1308
Size: 181 1127
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI AvaI BamHI BanII
BbsI BbvCI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsmI
Bsp1286I BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstYI
BtsI ClaI CspCI DraIII DrdI EaeI Eco57MI FalI
FseI Hin4I HincII MfeI MslI NaeI NgoMIV NspI
PasI PciI PfoI PpiI PspOMI ScaI SmaI SrfI
StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI BpuEI BsaAI BsaWI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrGI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI DraI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI
MscI MspA1I NarI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI Tth111I XbaI XcmI XhoI ZraI