Restriction map for
pF1KB6679
(Linear) (Six-base) MAPSORT of: KIBB6679.seq Check: 6818 from: 1 to: 1281
KIBB6679.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1281
Size: 505 776
AflIII A'CryG_T
Cuts at: 0 1118 1281
Size: 1118 163
AlwNI CAG_nnn'CTG
Cuts at: 0 186 1281
Size: 186 1095
ApoI r'AATT_y
Cuts at: 0 417 803 1195 1281
Size: 417 386 392 86
Fragments arranged by size:
417 392 386 86
AvaI C'yCGr_G
Cuts at: 0 94 1281
Size: 94 1187
BanI G'GyrC_C
Cuts at: 0 356 1281
Size: 356 925
BanII G_rGCy'C
Cuts at: 0 95 124 269 903 1281
Size: 95 29 145 634 378
Fragments arranged by size:
634 378 145 95 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 1281
Size: 37 1244
BglI GCCn_nnn'nGGC
Cuts at: 0 861 971 1281
Size: 861 110 310
Fragments arranged by size:
861 310 110
BmgBI CAC'GTC
Cuts at: 0 1119 1281
Size: 1119 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 488 948 1281
Size: 488 460 333
Fragments arranged by size:
488 460 333
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 922 1281
Size: 922 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 109 253 485 1272 1281
Size: 103 6 144 232 787 9
Fragments arranged by size:
787 232 144 103 9 6
BsaWI w'CCGG_w
Cuts at: 0 124 302 1281
Size: 124 178 979
Fragments arranged by size:
979 178 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 647 677 1281
Size: 60 30 557 30 604
Fragments arranged by size:
604 557 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 513 1281
Size: 81 432 768
Fragments arranged by size:
768 432 81
BseYI C'CCAG_C
Cuts at: 0 231 370 1281
Size: 231 139 911
Fragments arranged by size:
911 231 139
BsiEI CG_ry'CG
Cuts at: 0 1115 1281
Size: 1115 166
BsmI GAATG_Cn'
Cuts at: 0 722 1281
Size: 722 559
Bsp1286I G_dGCh'C
Cuts at: 0 95 124 269 903 1281
Size: 95 29 145 634 378
Fragments arranged by size:
634 378 145 95 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 821 1281
Size: 821 460
BsrDI GCAATG_nn'
Cuts at: 0 1235 1281
Size: 1235 46
BstEII G'GTnAC_C
Cuts at: 0 1234 1281
Size: 1234 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 1281
Size: 378 903
BstYI r'GATC_y
Cuts at: 0 182 932 1071 1281
Size: 182 750 139 210
Fragments arranged by size:
750 210 182 139
BtgI C'CryG_G
Cuts at: 0 377 914 1205 1281
Size: 377 537 291 76
Fragments arranged by size:
537 377 291 76
DraI TTT'AAA
Cuts at: 0 558 1281
Size: 558 723
EaeI y'GGCC_r
Cuts at: 0 247 773 1281
Size: 247 526 508
Fragments arranged by size:
526 508 247
EarI CTCTTCn'nnn_
Cuts at: 0 919 1281
Size: 919 362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 109 253 485 505 1272 1281
Size: 103 6 144 232 20 767 9
Fragments arranged by size:
767 232 144 103 20 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 330 817 1281
Size: 330 487 464
Fragments arranged by size:
487 464 330
EcoO109I rG'GnC_Cy
Cuts at: 0 212 810 1281
Size: 212 598 471
Fragments arranged by size:
598 471 212
EcoRI G'AATT_C
Cuts at: 0 1195 1281
Size: 1195 86
EcoRV GAT'ATC
Cuts at: 0 405 1281
Size: 405 876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 458 490 1117 1134 1149 1153
Size: 60 32 366 32 627 17 15 4
Cuts at: 1153 1166 1185 1281
Size: 13 19 96
Fragments arranged by size:
627 366 96 60 32 32 19 17
15 13 4
HincII GTy'rAC
Cuts at: 0 873 1281
Size: 873 408
MscI TGG'CCA
Cuts at: 0 249 775 1281
Size: 249 526 506
Fragments arranged by size:
526 506 249
MslI CAynn'nnrTG
Cuts at: 0 1054 1281
Size: 1054 227
NcoI C'CATG_G
Cuts at: 0 1205 1281
Size: 1205 76
NspI r_CATG'y
Cuts at: 0 742 1281
Size: 742 539
PasI CC'CwG_GG
Cuts at: 0 969 1281
Size: 969 312
PpuMI rG'GwC_Cy
Cuts at: 0 212 810 1281
Size: 212 598 471
Fragments arranged by size:
598 471 212
PvuI CG_AT'CG
Cuts at: 0 1115 1281
Size: 1115 166
SapI GCTCTTCn'nnn_
Cuts at: 0 919 1281
Size: 919 362
StyI C'CwwG_G
Cuts at: 0 347 1205 1281
Size: 347 858 76
Fragments arranged by size:
858 347 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 374 1029 1281
Size: 150 224 655 252
Fragments arranged by size:
655 252 224 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 647 679 1281
Size: 647 32 602
Fragments arranged by size:
647 602 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 761 1281
Size: 761 520
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BanI BanII BbsI
BglI BmgBI BmrI BplI BpmI BsaWI BsaXI BseRI
BseYI BsiEI BsmI Bsp1286I BspMI BsrDI BstEII BstXI
BstYI BtgI DraI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV Hin4I HincII MscI MslI NcoI NspI
PasI PpuMI PvuI SapI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI