Restriction map for
pF1KB6683
(Linear) (Six-base) MAPSORT of: KIBB6683.seq Check: 1248 from: 1 to: 1281
KIBB6683.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1060 1281
Size: 1060 221
ApoI r'AATT_y
Cuts at: 0 108 506 753 856 1281
Size: 108 398 247 103 425
Fragments arranged by size:
425 398 247 108 103
AvaI C'yCGr_G
Cuts at: 0 163 1281
Size: 163 1118
BanI G'GyrC_C
Cuts at: 0 73 1281
Size: 73 1208
BbeI G_GCGC'C
Cuts at: 0 77 1281
Size: 77 1204
BfrBI ATG'CAT
Cuts at: 0 350 401 1281
Size: 350 51 880
Fragments arranged by size:
880 350 51
BglI GCCn_nnn'nGGC
Cuts at: 0 15 1281
Size: 15 1266
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 1281
Size: 294 987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1281
Size: 999 282
Bpu10I CC'TnA_GC
Cuts at: 0 415 1281
Size: 415 866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1281
Size: 1093 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 1163 1281
Size: 1163 118
BsaHI Gr'CG_yC
Cuts at: 0 74 1281
Size: 74 1207
BsaWI w'CCGG_w
Cuts at: 0 237 1281
Size: 237 1044
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1174 1281
Size: 120 1054 107
Fragments arranged by size:
1054 120 107
BspEI T'CCGG_A
Cuts at: 0 237 1281
Size: 237 1044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1060 1281
Size: 1060 221
BsrDI GCAATG_nn'
Cuts at: 0 742 1281
Size: 742 539
BsrFI r'CCGG_y
Cuts at: 0 9 49 1281
Size: 9 40 1232
Fragments arranged by size:
1232 40 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 547 1281
Size: 547 734
DraI TTT'AAA
Cuts at: 0 1155 1281
Size: 1155 126
EaeI y'GGCC_r
Cuts at: 0 7 1281
Size: 7 1274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1281
Size: 999 282
EcoO109I rG'GnC_Cy
Cuts at: 0 296 1281
Size: 296 985
HaeII r_GCGC'y
Cuts at: 0 37 77 1281
Size: 37 40 1204
Fragments arranged by size:
1204 40 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 267 299 361 393 865 897
Size: 141 32 94 32 62 32 472 32
Cuts at: 897 1281
Size: 384
Fragments arranged by size:
472 384 141 94 62 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 73 1281
Size: 73 1208
MslI CAynn'nnrTG
Cuts at: 0 515 719 1039 1281
Size: 515 204 320 242
Fragments arranged by size:
515 320 242 204
NaeI GCC'GGC
Cuts at: 0 11 51 1281
Size: 11 40 1230
Fragments arranged by size:
1230 40 11
NarI GG'CG_CC
Cuts at: 0 74 1281
Size: 74 1207
NgoMIV G'CCGG_C
Cuts at: 0 9 49 1281
Size: 9 40 1232
Fragments arranged by size:
1232 40 9
NsiI A_TGCA'T
Cuts at: 0 352 403 1281
Size: 352 51 878
Fragments arranged by size:
878 352 51
PflMI CCAn_nnn'nTGG
Cuts at: 0 612 1281
Size: 612 669
PpuMI rG'GwC_Cy
Cuts at: 0 296 1281
Size: 296 985
SanDI GG'GwC_CC
Cuts at: 0 296 1281
Size: 296 985
ScaI AGT'ACT
Cuts at: 0 1085 1281
Size: 1085 196
SfcI C'TryA_G
Cuts at: 0 1244 1281
Size: 1244 37
SfoI GGC'GCC
Cuts at: 0 75 1281
Size: 75 1206
SgrAI Cr'CCGG_yG
Cuts at: 0 49 1281
Size: 49 1232
SmlI C'TyrA_G
Cuts at: 0 1108 1281
Size: 1108 173
StyI C'CwwG_G
Cuts at: 0 1226 1281
Size: 1226 55
TatI w'GTAC_w
Cuts at: 0 1083 1281
Size: 1083 198
XmnI GAAnn'nnTTC
Cuts at: 0 506 1281
Size: 506 775
Enzymes that do cut:
AarI ApoI AvaI BanI BbeI BfrBI BglI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsgI BspEI
BspMI BsrDI BsrFI BstXI DraI EaeI Eco57MI EcoO109I
HaeII Hin4I KasI MslI NaeI NarI NgoMIV NsiI
PflMI PpuMI SanDI ScaI SfcI SfoI SgrAI SmlI
StyI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmtI BplI BsaAI BsaBI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MspA1I NcoI NdeI NheI NotI NruI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI SexAI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI