Restriction map for
pF1KB6684
(Linear) (Six-base) MAPSORT of: KIBB6684.seq Check: 7701 from: 1 to: 1368
KIBB6684.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 373 383 977 1368
Size: 373 10 594 391
Fragments arranged by size:
594 391 373 10
AfeI AGC'GCT
Cuts at: 0 610 1368
Size: 610 758
AflIII A'CryG_T
Cuts at: 0 690 1368
Size: 690 678
ApaI G_GGCC'C
Cuts at: 0 507 1368
Size: 507 861
ApaLI G'TGCA_C
Cuts at: 0 790 1368
Size: 790 578
ApoI r'AATT_y
Cuts at: 0 816 1192 1368
Size: 816 376 176
Fragments arranged by size:
816 376 176
AvaI C'yCGr_G
Cuts at: 0 421 1368
Size: 421 947
BanI G'GyrC_C
Cuts at: 0 136 1368
Size: 136 1232
BanII G_rGCy'C
Cuts at: 0 507 1368
Size: 507 861
BbsI GAAGACnn'nnnn_
Cuts at: 0 1199 1368
Size: 1199 169
BclI T'GATC_A
Cuts at: 0 774 1368
Size: 774 594
BfrBI ATG'CAT
Cuts at: 0 1014 1368
Size: 1014 354
BglII A'GATC_T
Cuts at: 0 425 1368
Size: 425 943
BlpI GC'TnA_GC
Cuts at: 0 55 1368
Size: 55 1313
Bme1580I G_kGCm'C
Cuts at: 0 507 794 1368
Size: 507 287 574
Fragments arranged by size:
574 507 287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 646 1368
Size: 646 722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 832 1368
Size: 641 191 536
Fragments arranged by size:
641 536 191
Bpu10I CC'TnA_GC
Cuts at: 0 1084 1124 1368
Size: 1084 40 244
Fragments arranged by size:
1084 244 40
BsaWI w'CCGG_w
Cuts at: 0 1232 1368
Size: 1232 136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 1368
Size: 151 1217
BseYI C'CCAG_C
Cuts at: 0 456 546 1368
Size: 456 90 822
Fragments arranged by size:
822 456 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1242 1368
Size: 1242 126
BsiHKAI G_wGCw'C
Cuts at: 0 794 1002 1368
Size: 794 208 366
Fragments arranged by size:
794 366 208
BsmI GAATG_Cn'
Cuts at: 0 342 1368
Size: 342 1026
Bsp1286I G_dGCh'C
Cuts at: 0 507 794 1002 1368
Size: 507 287 208 366
Fragments arranged by size:
507 366 287 208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 1368
Size: 742 626
BsrBI CCG'CTC
Cuts at: 0 957 1368
Size: 957 411
BssSI C'ACGA_G
Cuts at: 0 588 1024 1368
Size: 588 436 344
Fragments arranged by size:
588 436 344
BstAPI GCAn_nnn'nTGC
Cuts at: 0 337 1368
Size: 337 1031
BstBI TT'CG_AA
Cuts at: 0 175 1368
Size: 175 1193
BstEII G'GTnAC_C
Cuts at: 0 1074 1368
Size: 1074 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 14 226 487 1368
Size: 14 212 261 881
Fragments arranged by size:
881 261 212 14
BstYI r'GATC_y
Cuts at: 0 425 1368
Size: 425 943
BtgI C'CryG_G
Cuts at: 0 264 353 1368
Size: 264 89 1015
Fragments arranged by size:
1015 264 89
BtsI GCAGTG_nn'
Cuts at: 0 216 1259 1368
Size: 216 1043 109
Fragments arranged by size:
1043 216 109
DrdI GACnn_nn'nnGTC
Cuts at: 0 703 1368
Size: 703 665
EarI CTCTTCn'nnn_
Cuts at: 0 538 599 637 1107 1368
Size: 538 61 38 470 261
Fragments arranged by size:
538 470 261 61 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 832 1368
Size: 641 191 536
Fragments arranged by size:
641 536 191
EcoNI CCTnn'n_nnAGG
Cuts at: 0 912 1368
Size: 912 456
EcoO109I rG'GnC_Cy
Cuts at: 0 10 39 503 1368
Size: 10 29 464 865
Fragments arranged by size:
865 464 29 10
EcoRI G'AATT_C
Cuts at: 0 816 1368
Size: 816 552
HaeII r_GCGC'y
Cuts at: 0 122 612 809 1368
Size: 122 490 197 559
Fragments arranged by size:
559 490 197 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 759 791 1368
Size: 759 32 577
Fragments arranged by size:
759 577 32
HincII GTy'rAC
Cuts at: 0 708 1368
Size: 708 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 446 666 1235 1368
Size: 42 404 220 569 133
Fragments arranged by size:
569 404 220 133 42
MspA1I CmG'CkG
Cuts at: 0 394 546 1368
Size: 394 152 822
Fragments arranged by size:
822 394 152
NcoI C'CATG_G
Cuts at: 0 264 1368
Size: 264 1104
NsiI A_TGCA'T
Cuts at: 0 1016 1368
Size: 1016 352
NspI r_CATG'y
Cuts at: 0 694 1014 1368
Size: 694 320 354
Fragments arranged by size:
694 354 320
PasI CC'CwG_GG
Cuts at: 0 14 1368
Size: 14 1354
PciI A'CATG_T
Cuts at: 0 690 1368
Size: 690 678
PfoI T'CCnGG_A
Cuts at: 0 295 1368
Size: 295 1073
PpuMI rG'GwC_Cy
Cuts at: 0 10 1368
Size: 10 1358
PspOMI G'GGCC_C
Cuts at: 0 503 1368
Size: 503 865
PvuII CAG'CTG
Cuts at: 0 394 546 1368
Size: 394 152 822
Fragments arranged by size:
822 394 152
SapI GCTCTTCn'nnn_
Cuts at: 0 538 599 1368
Size: 538 61 769
Fragments arranged by size:
769 538 61
SfcI C'TryA_G
Cuts at: 0 374 1368
Size: 374 994
SmlI C'TyrA_G
Cuts at: 0 421 1368
Size: 421 947
SphI G_CATG'C
Cuts at: 0 1014 1368
Size: 1014 354
StuI AGG'CCT
Cuts at: 0 563 859 1368
Size: 563 296 509
Fragments arranged by size:
563 509 296
StyI C'CwwG_G
Cuts at: 0 264 913 962 1368
Size: 264 649 49 406
Fragments arranged by size:
649 406 264 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 448 1368
Size: 21 427 920
Fragments arranged by size:
920 427 21
TatI w'GTAC_w
Cuts at: 0 584 1368
Size: 584 784
Tth111I GACn'n_nGTC
Cuts at: 0 875 1368
Size: 875 493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 724 1368
Size: 724 644
XhoI C'TCGA_G
Cuts at: 0 421 1368
Size: 421 947
Enzymes that do cut:
AccI AfeI AflIII ApaI ApaLI ApoI AvaI BanI
BanII BbsI BclI BfrBI BglII BlpI Bme1580I BplI
BpmI Bpu10I BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtsI DrdI EarI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII MmeI MspA1I NcoI NsiI
NspI PasI PciI PfoI PpuMI PspOMI PvuII SapI
SfcI SmlI SphI StuI StyI TaqII TatI Tth111I
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BglI BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EaeI EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TstI XbaI XmaI
XmnI ZraI