Restriction map for
pF1KB6685
(Linear) (Six-base) MAPSORT of: KIBB6685.seq Check: 5480 from: 1 to: 1281
KIBB6685.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 614 1281
Size: 614 667
AccI GT'mk_AC
Cuts at: 0 1265 1281
Size: 1265 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1281
Size: 466 815
AflIII A'CryG_T
Cuts at: 0 411 1281
Size: 411 870
AlwNI CAG_nnn'CTG
Cuts at: 0 117 1281
Size: 117 1164
ApoI r'AATT_y
Cuts at: 0 460 924 1156 1281
Size: 460 464 232 125
Fragments arranged by size:
464 460 232 125
AvaI C'yCGr_G
Cuts at: 0 210 569 1281
Size: 210 359 712
Fragments arranged by size:
712 359 210
BanI G'GyrC_C
Cuts at: 0 726 1120 1281
Size: 726 394 161
Fragments arranged by size:
726 394 161
BanII G_rGCy'C
Cuts at: 0 136 602 1281
Size: 136 466 679
Fragments arranged by size:
679 466 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1245 1279 1281
Size: 1245 34 2
Fragments arranged by size:
1245 34 2
BfrBI ATG'CAT
Cuts at: 0 3 1281
Size: 3 1278
BlpI GC'TnA_GC
Cuts at: 0 238 1281
Size: 238 1043
Bme1580I G_kGCm'C
Cuts at: 0 532 731 1281
Size: 532 199 550
Fragments arranged by size:
550 532 199
BmgBI CAC'GTC
Cuts at: 0 555 1107 1281
Size: 555 552 174
Fragments arranged by size:
555 552 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 866 1090 1281
Size: 139 727 224 191
Fragments arranged by size:
727 224 191 139
Bpu10I CC'TnA_GC
Cuts at: 0 433 1281
Size: 433 848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 325 586 660 1281
Size: 130 195 261 74 621
Fragments arranged by size:
621 261 195 130 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 1281
Size: 199 1082
BsaHI Gr'CG_yC
Cuts at: 0 611 1281
Size: 611 670
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 1281
Size: 13 1268
BseYI C'CCAG_C
Cuts at: 0 139 1005 1281
Size: 139 866 276
Fragments arranged by size:
866 276 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 358 1281
Size: 49 309 923
Fragments arranged by size:
923 309 49
BsiHKAI G_wGCw'C
Cuts at: 0 426 602 1281
Size: 426 176 679
Fragments arranged by size:
679 426 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 809 1281
Size: 809 472
Bsp1286I G_dGCh'C
Cuts at: 0 136 426 532 602 731 1281
Size: 136 290 106 70 129 550
Fragments arranged by size:
550 290 136 129 106 70
BspHI T'CATG_A
Cuts at: 0 768 1281
Size: 768 513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 1281
Size: 26 1255
BsrFI r'CCGG_y
Cuts at: 0 1199 1281
Size: 1199 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 372 1281
Size: 372 909
BstYI r'GATC_y
Cuts at: 0 299 1017 1281
Size: 299 718 264
Fragments arranged by size:
718 299 264
BtgI C'CryG_G
Cuts at: 0 396 476 1281
Size: 396 80 805
Fragments arranged by size:
805 396 80
BtsI GCAGTG_nn'
Cuts at: 0 426 1281
Size: 426 855
EaeI y'GGCC_r
Cuts at: 0 57 393 479 1281
Size: 57 336 86 802
Fragments arranged by size:
802 336 86 57
EarI CTCTTCn'nnn_
Cuts at: 0 505 863 913 1281
Size: 505 358 50 368
Fragments arranged by size:
505 368 358 50
EcoICRI GAG'CTC
Cuts at: 0 600 1281
Size: 600 681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 466 866 1090 1281
Size: 139 327 400 224 191
Fragments arranged by size:
400 327 224 191 139
EcoNI CCTnn'n_nnAGG
Cuts at: 0 845 1281
Size: 845 436
EcoO109I rG'GnC_Cy
Cuts at: 0 275 309 1281
Size: 275 34 972
Fragments arranged by size:
972 275 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 240 630 1116 1281
Size: 240 390 486 165
Fragments arranged by size:
486 390 240 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1281
Size: 1009 32 240
Fragments arranged by size:
1009 240 32
HincII GTy'rAC
Cuts at: 0 1266 1281
Size: 1266 15
MluI A'CGCG_T
Cuts at: 0 411 1281
Size: 411 870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1281
Size: 15 1266
MscI TGG'CCA
Cuts at: 0 59 1281
Size: 59 1222
MslI CAynn'nnrTG
Cuts at: 0 80 1281
Size: 80 1201
MspA1I CmG'CkG
Cuts at: 0 63 237 1281
Size: 63 174 1044
Fragments arranged by size:
1044 174 63
NcoI C'CATG_G
Cuts at: 0 396 476 1281
Size: 396 80 805
Fragments arranged by size:
805 396 80
NruI TCG'CGA
Cuts at: 0 1150 1281
Size: 1150 131
NsiI A_TGCA'T
Cuts at: 0 5 1281
Size: 5 1276
PasI CC'CwG_GG
Cuts at: 0 270 1281
Size: 270 1011
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 1281
Size: 402 879
PspXI vC'TCGA_Gb
Cuts at: 0 569 1281
Size: 569 712
PstI C_TGCA'G
Cuts at: 0 743 1134 1281
Size: 743 391 147
Fragments arranged by size:
743 391 147
PvuII CAG'CTG
Cuts at: 0 63 1281
Size: 63 1218
SacI G_AGCT'C
Cuts at: 0 602 1281
Size: 602 679
SalI G'TCGA_C
Cuts at: 0 1264 1281
Size: 1264 17
SfcI C'TryA_G
Cuts at: 0 739 1130 1281
Size: 739 391 151
Fragments arranged by size:
739 391 151
SmaI CCC'GGG
Cuts at: 0 212 1281
Size: 212 1069
SmlI C'TyrA_G
Cuts at: 0 145 304 569 601 675 1281
Size: 145 159 265 32 74 606
Fragments arranged by size:
606 265 159 145 74 32
StyI C'CwwG_G
Cuts at: 0 396 476 846 1100 1281
Size: 396 80 370 254 181
Fragments arranged by size:
396 370 254 181 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1249 1281
Size: 1249 32
Tth111I GACn'n_nGTC
Cuts at: 0 553 1281
Size: 553 728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 1104 1281
Size: 267 837 177
Fragments arranged by size:
837 267 177
XhoI C'TCGA_G
Cuts at: 0 569 1281
Size: 569 712
XmaI C'CCGG_G
Cuts at: 0 210 1281
Size: 210 1071
XmnI GAAnn'nnTTC
Cuts at: 0 664 872 1281
Size: 664 208 409
Fragments arranged by size:
664 409 208
ZraI GAC'GTC
Cuts at: 0 612 1281
Size: 612 669
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApoI AvaI BanI
BanII BcgI BfrBI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BstAPI BstYI BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII MluI MmeI MscI MslI MspA1I NcoI NruI
NsiI PasI PflMI PspXI PstI PvuII SacI SalI
SfcI SmaI SmlI StyI TaqII Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BglI
BglII BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI NaeI NarI NdeI NgoMIV NheI NotI NspI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
XbaI