(Linear) (Six-base) MAPSORT of: KIBB6685.seq Check: 5480 from: 1 to: 1281 KIBB6685.orf With 182 enzymes: SgfI * October 7, 2009 14:22 .. AatII G_ACGT'C Cuts at: 0 614 1281 Size: 614 667 AccI GT'mk_AC Cuts at: 0 1265 1281 Size: 1265 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 1281 Size: 466 815 AflIII A'CryG_T Cuts at: 0 411 1281 Size: 411 870 AlwNI CAG_nnn'CTG Cuts at: 0 117 1281 Size: 117 1164 ApoI r'AATT_y Cuts at: 0 460 924 1156 1281 Size: 460 464 232 125 Fragments arranged by size: 464 460 232 125 AvaI C'yCGr_G Cuts at: 0 210 569 1281 Size: 210 359 712 Fragments arranged by size: 712 359 210 BanI G'GyrC_C Cuts at: 0 726 1120 1281 Size: 726 394 161 Fragments arranged by size: 726 394 161 BanII G_rGCy'C Cuts at: 0 136 602 1281 Size: 136 466 679 Fragments arranged by size: 679 466 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1245 1279 1281 Size: 1245 34 2 Fragments arranged by size: 1245 34 2 BfrBI ATG'CAT Cuts at: 0 3 1281 Size: 3 1278 BlpI GC'TnA_GC Cuts at: 0 238 1281 Size: 238 1043 Bme1580I G_kGCm'C Cuts at: 0 532 731 1281 Size: 532 199 550 Fragments arranged by size: 550 532 199 BmgBI CAC'GTC Cuts at: 0 555 1107 1281 Size: 555 552 174 Fragments arranged by size: 555 552 174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 866 1090 1281 Size: 139 727 224 191 Fragments arranged by size: 727 224 191 139 Bpu10I CC'TnA_GC Cuts at: 0 433 1281 Size: 433 848 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 325 586 660 1281 Size: 130 195 261 74 621 Fragments arranged by size: 621 261 195 130 74 BsaI GGTCTCn'nnnn_ Cuts at: 0 199 1281 Size: 199 1082 BsaHI Gr'CG_yC Cuts at: 0 611 1281 Size: 611 670 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 13 1281 Size: 13 1268 BseYI C'CCAG_C Cuts at: 0 139 1005 1281 Size: 139 866 276 Fragments arranged by size: 866 276 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 358 1281 Size: 49 309 923 Fragments arranged by size: 923 309 49 BsiHKAI G_wGCw'C Cuts at: 0 426 602 1281 Size: 426 176 679 Fragments arranged by size: 679 426 176 BsmBI CGTCTCn'nnnn_ Cuts at: 0 809 1281 Size: 809 472 Bsp1286I G_dGCh'C Cuts at: 0 136 426 532 602 731 1281 Size: 136 290 106 70 129 550 Fragments arranged by size: 550 290 136 129 106 70 BspHI T'CATG_A Cuts at: 0 768 1281 Size: 768 513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 26 1281 Size: 26 1255 BsrFI r'CCGG_y Cuts at: 0 1199 1281 Size: 1199 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 372 1281 Size: 372 909 BstYI r'GATC_y Cuts at: 0 299 1017 1281 Size: 299 718 264 Fragments arranged by size: 718 299 264 BtgI C'CryG_G Cuts at: 0 396 476 1281 Size: 396 80 805 Fragments arranged by size: 805 396 80 BtsI GCAGTG_nn' Cuts at: 0 426 1281 Size: 426 855 EaeI y'GGCC_r Cuts at: 0 57 393 479 1281 Size: 57 336 86 802 Fragments arranged by size: 802 336 86 57 EarI CTCTTCn'nnn_ Cuts at: 0 505 863 913 1281 Size: 505 358 50 368 Fragments arranged by size: 505 368 358 50 EcoICRI GAG'CTC Cuts at: 0 600 1281 Size: 600 681 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 466 866 1090 1281 Size: 139 327 400 224 191 Fragments arranged by size: 400 327 224 191 139 EcoNI CCTnn'n_nnAGG Cuts at: 0 845 1281 Size: 845 436 EcoO109I rG'GnC_Cy Cuts at: 0 275 309 1281 Size: 275 34 972 Fragments arranged by size: 972 275 34 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 240 630 1116 1281 Size: 240 390 486 165 Fragments arranged by size: 486 390 240 165 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1009 1041 1281 Size: 1009 32 240 Fragments arranged by size: 1009 240 32 HincII GTy'rAC Cuts at: 0 1266 1281 Size: 1266 15 MluI A'CGCG_T Cuts at: 0 411 1281 Size: 411 870 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 15 1281 Size: 15 1266 MscI TGG'CCA Cuts at: 0 59 1281 Size: 59 1222 MslI CAynn'nnrTG Cuts at: 0 80 1281 Size: 80 1201 MspA1I CmG'CkG Cuts at: 0 63 237 1281 Size: 63 174 1044 Fragments arranged by size: 1044 174 63 NcoI C'CATG_G Cuts at: 0 396 476 1281 Size: 396 80 805 Fragments arranged by size: 805 396 80 NruI TCG'CGA Cuts at: 0 1150 1281 Size: 1150 131 NsiI A_TGCA'T Cuts at: 0 5 1281 Size: 5 1276 PasI CC'CwG_GG Cuts at: 0 270 1281 Size: 270 1011 PflMI CCAn_nnn'nTGG Cuts at: 0 402 1281 Size: 402 879 PspXI vC'TCGA_Gb Cuts at: 0 569 1281 Size: 569 712 PstI C_TGCA'G Cuts at: 0 743 1134 1281 Size: 743 391 147 Fragments arranged by size: 743 391 147 PvuII CAG'CTG Cuts at: 0 63 1281 Size: 63 1218 SacI G_AGCT'C Cuts at: 0 602 1281 Size: 602 679 SalI G'TCGA_C Cuts at: 0 1264 1281 Size: 1264 17 SfcI C'TryA_G Cuts at: 0 739 1130 1281 Size: 739 391 151 Fragments arranged by size: 739 391 151 SmaI CCC'GGG Cuts at: 0 212 1281 Size: 212 1069 SmlI C'TyrA_G Cuts at: 0 145 304 569 601 675 1281 Size: 145 159 265 32 74 606 Fragments arranged by size: 606 265 159 145 74 32 StyI C'CwwG_G Cuts at: 0 396 476 846 1100 1281 Size: 396 80 370 254 181 Fragments arranged by size: 396 370 254 181 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1249 1281 Size: 1249 32 Tth111I GACn'n_nGTC Cuts at: 0 553 1281 Size: 553 728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 267 1104 1281 Size: 267 837 177 Fragments arranged by size: 837 267 177 XhoI C'TCGA_G Cuts at: 0 569 1281 Size: 569 712 XmaI C'CCGG_G Cuts at: 0 210 1281 Size: 210 1071 XmnI GAAnn'nnTTC Cuts at: 0 664 872 1281 Size: 664 208 409 Fragments arranged by size: 664 409 208 ZraI GAC'GTC Cuts at: 0 612 1281 Size: 612 669 Enzymes that do cut: AatII AccI AcuI AflIII AlwNI ApoI AvaI BanI BanII BcgI BfrBI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BstAPI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MluI MmeI MscI MslI MspA1I NcoI NruI NsiI PasI PflMI PspXI PstI PvuII SacI SalI SfcI SmaI SmlI StyI TaqII Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BglI BglII BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI NaeI NarI NdeI NgoMIV NheI NotI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI