Restriction map for
pF1KB6693
(Linear) (Six-base) MAPSORT of: KIBB6693.seq Check: 4223 from: 1 to: 1287
KIBB6693.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 31 300 1287
Size: 31 269 987
Fragments arranged by size:
987 269 31
AclI AA'CG_TT
Cuts at: 0 626 1287
Size: 626 661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 217 476 1168 1237 1287
Size: 172 45 259 692 69 50
Fragments arranged by size:
692 259 172 69 50 45
AflIII A'CryG_T
Cuts at: 0 116 254 426 732 1287
Size: 116 138 172 306 555
Fragments arranged by size:
555 306 172 138 116
AleI CACnn'nnGTG
Cuts at: 0 815 1287
Size: 815 472
AlwNI CAG_nnn'CTG
Cuts at: 0 706 1287
Size: 706 581
ApaI G_GGCC'C
Cuts at: 0 101 1287
Size: 101 1186
ApoI r'AATT_y
Cuts at: 0 577 936 1287
Size: 577 359 351
Fragments arranged by size:
577 359 351
AseI AT'TA_AT
Cuts at: 0 284 1287
Size: 284 1003
AvrII C'CTAG_G
Cuts at: 0 52 1287
Size: 52 1235
BanI G'GyrC_C
Cuts at: 0 48 1287
Size: 48 1239
BanII G_rGCy'C
Cuts at: 0 101 1287
Size: 101 1186
BfrBI ATG'CAT
Cuts at: 0 1248 1287
Size: 1248 39
Bme1580I G_kGCm'C
Cuts at: 0 101 1287
Size: 101 1186
BmrI ACTGGGnnnn_n'
Cuts at: 0 499 1287
Size: 499 788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 947 952 1287
Size: 299 648 5 335
Fragments arranged by size:
648 335 299 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 835 1287
Size: 835 452
BsaHI Gr'CG_yC
Cuts at: 0 28 297 1287
Size: 28 269 990
Fragments arranged by size:
990 269 28
BsaWI w'CCGG_w
Cuts at: 0 182 1287
Size: 182 1105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 413 443 1287
Size: 413 30 844
Fragments arranged by size:
844 413 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 187 1024 1025 1287
Size: 184 3 837 1 262
Fragments arranged by size:
837 262 184 3 1
BseYI C'CCAG_C
Cuts at: 0 968 1287
Size: 968 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1287
Size: 977 310
BsiHKAI G_wGCw'C
Cuts at: 0 1113 1287
Size: 1113 174
Bsp1286I G_dGCh'C
Cuts at: 0 101 1113 1287
Size: 101 1012 174
Fragments arranged by size:
1012 174 101
BspEI T'CCGG_A
Cuts at: 0 182 1287
Size: 182 1105
BsrBI CCG'CTC
Cuts at: 0 93 1287
Size: 93 1194
BtgI C'CryG_G
Cuts at: 0 60 1287
Size: 60 1227
BtsI GCAGTG_nn'
Cuts at: 0 223 1287
Size: 223 1064
DraI TTT'AAA
Cuts at: 0 632 1287
Size: 632 655
EaeI y'GGCC_r
Cuts at: 0 14 1287
Size: 14 1273
EarI CTCTTCn'nnn_
Cuts at: 0 181 584 1287
Size: 181 403 703
Fragments arranged by size:
703 403 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 217 476 1168 1237 1287
Size: 172 45 259 692 69 50
Fragments arranged by size:
692 259 172 69 50 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 489 1039 1287
Size: 489 550 248
Fragments arranged by size:
550 489 248
EcoO109I rG'GnC_Cy
Cuts at: 0 56 97 201 1287
Size: 56 41 104 1086
Fragments arranged by size:
1086 104 56 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 352 384 1287
Size: 26 326 32 903
Fragments arranged by size:
903 326 32 26
HincII GTy'rAC
Cuts at: 0 795 1287
Size: 795 492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1287
Size: 1253 34
MslI CAynn'nnrTG
Cuts at: 0 121 815 1287
Size: 121 694 472
Fragments arranged by size:
694 472 121
MspA1I CmG'CkG
Cuts at: 0 972 1287
Size: 972 315
NsiI A_TGCA'T
Cuts at: 0 1250 1287
Size: 1250 37
NspI r_CATG'y
Cuts at: 0 120 258 430 1248 1252 1287
Size: 120 138 172 818 4 35
Fragments arranged by size:
818 172 138 120 35 4
PciI A'CATG_T
Cuts at: 0 116 254 426 1287
Size: 116 138 172 861
Fragments arranged by size:
861 172 138 116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 1287
Size: 160 32 1095
Fragments arranged by size:
1095 160 32
PpuMI rG'GwC_Cy
Cuts at: 0 56 1287
Size: 56 1231
PspOMI G'GGCC_C
Cuts at: 0 97 1287
Size: 97 1190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 422 454 722 754 1184 1216 1287
Size: 422 32 268 32 430 32 71
Fragments arranged by size:
430 422 268 71 32 32 32
PstI C_TGCA'G
Cuts at: 0 387 711 1287
Size: 387 324 576
Fragments arranged by size:
576 387 324
PvuII CAG'CTG
Cuts at: 0 972 1287
Size: 972 315
SapI GCTCTTCn'nnn_
Cuts at: 0 181 1287
Size: 181 1106
SfcI C'TryA_G
Cuts at: 0 383 707 1160 1287
Size: 383 324 453 127
Fragments arranged by size:
453 383 324 127
SmlI C'TyrA_G
Cuts at: 0 314 931 962 1287
Size: 314 617 31 325
Fragments arranged by size:
617 325 314 31
SphI G_CATG'C
Cuts at: 0 1252 1287
Size: 1252 35
StyI C'CwwG_G
Cuts at: 0 52 845 1287
Size: 52 793 442
Fragments arranged by size:
793 442 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 356 1287
Size: 356 931
TatI w'GTAC_w
Cuts at: 0 262 735 914 1287
Size: 262 473 179 373
Fragments arranged by size:
473 373 262 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1245 1277 1287
Size: 1245 32 10
Fragments arranged by size:
1245 32 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 814 852 1003 1287
Size: 814 38 151 284
Fragments arranged by size:
814 284 151 38
ZraI GAC'GTC
Cuts at: 0 29 298 1287
Size: 29 269 989
Fragments arranged by size:
989 269 29
Enzymes that do cut:
AatII AclI AcuI AflIII AleI AlwNI ApaI ApoI
AseI AvrII BanI BanII BfrBI Bme1580I BmrI BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BsrBI BtgI BtsI DraI EaeI EarI
Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MslI MspA1I
NsiI NspI PciI PpiI PpuMI PspOMI PsrI PstI
PvuII SapI SfcI SmlI SphI StyI TaqII TatI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvaI BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmgBI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsiEI
BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI