Restriction map for
pF1KB6696
(Linear) (Six-base) MAPSORT of: KIBB6696.seq Check: 6987 from: 1 to: 1386
KIBB6696.orf
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 907 1215 1386
Size: 761 146 308 171
Fragments arranged by size:
761 308 171 146
AfeI AGC'GCT
Cuts at: 0 198 1386
Size: 198 1188
AflIII A'CryG_T
Cuts at: 0 641 1386
Size: 641 745
AhdI GACnn_n'nnGTC
Cuts at: 0 374 1386
Size: 374 1012
AleI CACnn'nnGTG
Cuts at: 0 161 1386
Size: 161 1225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 179 1386
Size: 179 1207
AlwNI CAG_nnn'CTG
Cuts at: 0 84 1372 1386
Size: 84 1288 14
Fragments arranged by size:
1288 84 14
ApoI r'AATT_y
Cuts at: 0 1286 1386
Size: 1286 100
BamHI G'GATC_C
Cuts at: 0 767 1386
Size: 767 619
BanI G'GyrC_C
Cuts at: 0 163 1386
Size: 163 1223
BanII G_rGCy'C
Cuts at: 0 440 1386
Size: 440 946
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 781 1386
Size: 496 285 605
Fragments arranged by size:
605 496 285
BbvCI CC'TCA_GC
Cuts at: 0 110 1386
Size: 110 1276
BciVI GTATCCnnnnn_n'
Cuts at: 0 1255 1386
Size: 1255 131
BclI T'GATC_A
Cuts at: 0 386 1386
Size: 386 1000
BfrBI ATG'CAT
Cuts at: 0 1201 1386
Size: 1201 185
BglI GCCn_nnn'nGGC
Cuts at: 0 172 1386
Size: 172 1214
BglII A'GATC_T
Cuts at: 0 890 1386
Size: 890 496
BlpI GC'TnA_GC
Cuts at: 0 11 1386
Size: 11 1375
Bme1580I G_kGCm'C
Cuts at: 0 168 364 1386
Size: 168 196 1022
Fragments arranged by size:
1022 196 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 882 1183 1386
Size: 882 301 203
Fragments arranged by size:
882 301 203
Bpu10I CC'TnA_GC
Cuts at: 0 110 537 1386
Size: 110 427 849
Fragments arranged by size:
849 427 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 952 1386
Size: 228 724 434
Fragments arranged by size:
724 434 228
BsaI GGTCTCn'nnnn_
Cuts at: 0 1298 1386
Size: 1298 88
BsaBI GATnn'nnATC
Cuts at: 0 619 1386
Size: 619 767
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1297 1327 1386
Size: 1297 30 59
Fragments arranged by size:
1297 59 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1386
Size: 28 1358
BseYI C'CCAG_C
Cuts at: 0 255 262 1386
Size: 255 7 1124
Fragments arranged by size:
1124 255 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 1386
Size: 651 735
BsiEI CG_ry'CG
Cuts at: 0 176 371 1386
Size: 176 195 1015
Fragments arranged by size:
1015 195 176
BsiHKAI G_wGCw'C
Cuts at: 0 143 929 1386
Size: 143 786 457
Fragments arranged by size:
786 457 143
Bsp1286I G_dGCh'C
Cuts at: 0 143 168 364 440 929 1386
Size: 143 25 196 76 489 457
Fragments arranged by size:
489 457 196 143 76 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 890 1386
Size: 890 496
BsrDI GCAATG_nn'
Cuts at: 0 689 1154 1386
Size: 689 465 232
Fragments arranged by size:
689 465 232
BsrFI r'CCGG_y
Cuts at: 0 370 1386
Size: 370 1016
BsrGI T'GTAC_A
Cuts at: 0 841 1386
Size: 841 545
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1372 1386
Size: 1372 14
BstBI TT'CG_AA
Cuts at: 0 1017 1386
Size: 1017 369
BstXI CCAn_nnnn'nTGG
Cuts at: 0 298 1329 1386
Size: 298 1031 57
Fragments arranged by size:
1031 298 57
BstYI r'GATC_y
Cuts at: 0 767 890 1386
Size: 767 123 496
Fragments arranged by size:
767 496 123
BtgI C'CryG_G
Cuts at: 0 185 1316 1386
Size: 185 1131 70
Fragments arranged by size:
1131 185 70
ClaI AT'CG_AT
Cuts at: 0 684 1386
Size: 684 702
EaeI y'GGCC_r
Cuts at: 0 173 392 589 1319 1386
Size: 173 219 197 730 67
Fragments arranged by size:
730 219 197 173 67
EagI C'GGCC_G
Cuts at: 0 173 1386
Size: 173 1213
EarI CTCTTCn'nnn_
Cuts at: 0 445 780 1260 1386
Size: 445 335 480 126
Fragments arranged by size:
480 445 335 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 16 1386
Size: 16 1370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 907 1215 1386
Size: 761 146 308 171
Fragments arranged by size:
761 308 171 146
EcoRI G'AATT_C
Cuts at: 0 1286 1386
Size: 1286 100
HaeII r_GCGC'y
Cuts at: 0 118 200 1386
Size: 118 82 1186
Fragments arranged by size:
1186 118 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 360 392 666 698 941 973 1297 1329
Size: 360 32 274 32 243 32 324 32
Cuts at: 1329 1386
Size: 57
Fragments arranged by size:
360 324 274 243 57 32 32 32
32
HincII GTy'rAC
Cuts at: 0 282 1386
Size: 282 1104
MfeI C'AATT_G
Cuts at: 0 219 1386
Size: 219 1167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 391 886 1283 1386
Size: 335 56 495 397 103
Fragments arranged by size:
495 397 335 103 56
MscI TGG'CCA
Cuts at: 0 591 1386
Size: 591 795
MslI CAynn'nnrTG
Cuts at: 0 161 296 403 463 484 937 1030 1386
Size: 161 135 107 60 21 453 93 356
Fragments arranged by size:
453 356 161 135 107 93 60 21
MspA1I CmG'CkG
Cuts at: 0 93 161 1178 1386
Size: 93 68 1017 208
Fragments arranged by size:
1017 208 93 68
NcoI C'CATG_G
Cuts at: 0 185 1386
Size: 185 1201
NsiI A_TGCA'T
Cuts at: 0 1203 1386
Size: 1203 183
NspI r_CATG'y
Cuts at: 0 645 984 1386
Size: 645 339 402
Fragments arranged by size:
645 402 339
PciI A'CATG_T
Cuts at: 0 641 1386
Size: 641 745
PvuII CAG'CTG
Cuts at: 0 1178 1386
Size: 1178 208
RsrII CG'GwC_CG
Cuts at: 0 322 1386
Size: 322 1064
SapI GCTCTTCn'nnn_
Cuts at: 0 445 1386
Size: 445 941
SexAI A'CCwGG_T
Cuts at: 0 865 1386
Size: 865 521
SmlI C'TyrA_G
Cuts at: 0 243 967 1386
Size: 243 724 419
Fragments arranged by size:
724 419 243
SphI G_CATG'C
Cuts at: 0 984 1386
Size: 984 402
StuI AGG'CCT
Cuts at: 0 715 1386
Size: 715 671
StyI C'CwwG_G
Cuts at: 0 185 233 1103 1386
Size: 185 48 870 283
Fragments arranged by size:
870 283 185 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7 276 1386
Size: 7 269 1110
Fragments arranged by size:
1110 269 7
TatI w'GTAC_w
Cuts at: 0 841 1386
Size: 841 545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 426 869 1035 1386
Size: 402 24 443 166 351
Fragments arranged by size:
443 402 351 166 24
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApoI
BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI
BtgI ClaI EaeI EagI EarI EciI Eco57MI EcoRI
HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PvuII RsrII SapI SexAI
SmlI SphI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BmgBI BmtI BplI BpmI BsaAI BsaHI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI SacI SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI